LOCUS       pRO286-amtRO7           1848 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            mNeonGreen_in_yeast_moclo_vector.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    605..610
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    612..615
                     /label="part2_fw_overlap_yeast_moclo"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    618..638
                     /label="amtRO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    641..661
                     /label="amtRO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    664..684
                     /label="amtRO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    687..707
                     /label="amtRO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    710..730
                     /label="amtRO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    733..753
                     /label="amtRO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    756..776
                     /label="amtRO"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    798..801
                     /label="part2A_rv_overlap_yeast_moclo"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(803..808)
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Antibiotic r... complement(811..919)
                     /label="CmR Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(920..1579)
                     /label="CmR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(1580..1684)
                     /label="CmR Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(1685..600)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
       61 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      121 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      181 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      241 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      301 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      361 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      421 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      481 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      541 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      601 agTCGGTCTC AAACGGGTTC TATCGATCTA TAGATAATGA TTCTATCGAT CTATAGATAA
      661 TCATTCTATC GATCTATAGA TAATCATTCT ATCGATCTAT AGATAATGTT TCTATCGATC
      721 TATAGATAAT AGTTCTATCG ATCTATAGAT AATCGTTCTA TCGATCTATA GATAATCGGA
      781 TCTCAGCTAG CTAGATCTTG ATGAGACCag accaataaaa aacgcccggc ggcaaccgag
      841 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
      901 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
      961 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1021 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1081 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1141 ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1201 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1261 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1321 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1381 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1441 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1501 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1561 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1621 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     1681 tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1741 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1801 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagag
//