LOCUS       pRO271-AmtR-NES          753 bp ds-DNA     linear       06-DEC-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            pRO271_AmtR_3a_working.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    5..10
                     /label="BsmBi"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="BsmBi"
                     /label="BsmBi"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    14..19
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    21..24
                     /label="type_3_fw"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /locus_tag="type_3_fw"
                     /label="type_3_fw"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    25..693
                     /label="AmtR_yeastIDTcodonoptimized"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="AmtR_yeastIDTcodonoptimized"
                     /label="AmtR_yeastIDTcodonoptimized"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    694..702
                     /label="Gly-Ser-Gly"
                     /ApEinfo_revcolor="#ff0846"
                     /ApEinfo_fwdcolor="#ff0846"
                     /locus_tag="Gly-Ser-Gly"
                     /label="Gly-Ser-Gly"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    703..729
                     /label="nuclear_export_signal_IDTyeastcodonoptimized_from_a ddgeneplasmid_42608"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="nuclear_export_signal_IDTyeastcodonoptimized_from_a ddgeneplasmid_42608"
                     /label="nuclear_export_signal_IDTyeastcodonoptimized_from_a ddgeneplasmid_42608"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    732..735
                     /label="3a rv"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="3a rv"
                     /label="3a rv"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(737..742)
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="BsaI(1)"
                     /label="BsaI(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(744..749)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="BsmBI"
                     /label="BsmBI"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 GCATCGTCTC ATCGGTCTCA TATGGCTGGT GCTGTGGGAA GGCCAAGGAG GTCCGCCCCC
       61 AGGAGAGCGG GGAAAAATCC TCGTGAAGAG ATACTGGACG CATCTGCTGA GCTTTTTACG
      121 CGTCAAGGCT TTGCTACTAC TTCAACTCAC CAGATAGCAG ATGCGGTCGG CATTAGACAG
      181 GCTTCTCTAT ATTATCATTT TCCGTCAAAA ACGGAGATTT TCCTTACACT ACTTAAGTCT
      241 ACAGTTGAGC CTTCAACTGT CTTGGCAGAG GATCTATCCA CCCTTGACGC CGGTCCCGAG
      301 ATGAGATTGT GGGCTATCGT AGCGAGTGAG GTGCGTTTAT TGCTATCTAC AAAATGGAAT
      361 GTAGGAAGGC TATACCAATT GCCTATCGTG GGCTCAGAGG AGTTCGCTGA ATACCATTCC
      421 CAGAGGGAGG CGCTTACAAA TGTCTTTCGT GATCTTGCTA CAGAAATTGT AGGAGATGAC
      481 CCCAGAGCGG AGCTGCCCTT CCACATAACT ATGAGCGTGA TAGAGATGCG TAGGAACGAT
      541 GGAAAGATTC CAAGTCCATT AAGTGCAGAT AGTCTTCCAG AAACCGCAAT CATGTTGGCT
      601 GACGCCAGCT TGGCCGTCCT GGGAGCACCC CTACCGGCCG ATAGGGTTGA AAAGACGCTT
      661 GAATTGATTA AACAGGCAGA CGCAAAGGCA AGCggaagcg gaCTGCCTCC GTTAGAAAGA
      721 CTAACGCTGG GTTCTTGAGA CCTGAGACGG CAT
//