LOCUS       pWN043-DNCR2            2355 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     Imported using the Genbank importer. File name: pWN043.gb
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label="BsaI site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="BsaI site(1)"
                     /label="BsaI site(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    10..13
                     /label="3b"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /locus_tag="3b(2)"
                     /label="3b(2)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    14..700
                     /label="DNCR2_IDT_codon_optimize_fromaddgene133607"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /locus_tag="DNCR_IDT_codon_optimize_fromaddgene133607"
                     /label="DNCR_IDT_codon_optimize_fromaddgene133607"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    703..706
                     /label="3b"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /locus_tag="3b"
                     /label="3b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    708..713
                     /label="BsaI site"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="BsaI site"
                     /label="BsaI site"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     Antibiotic r... complement(716..824)
                     /label="CmR Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(825..1484)
                     /label="CmR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(825..1484)
                     /label="CamR"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
                     /locus_tag="CamR"
                     /label="CamR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
     Antibiotic r... complement(1485..1589)
                     /label="CmR Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(1590..2353)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 tcGGTCTCaT TCTTCTTCCG ATGAAGAGGA AGCTAGAGAG CTAATCGAAA GAGCGAAGGA
       61 GGCAGCCGAA AGAGCTCAAG AGGCCGCTGA AAGGACCGGT GATCCGAGAG TGAGAGAATT
      121 GGCCAGAGAA CTGAAGAGGT TGGCCCAGGA AGCTGCTGAG GAAGTCAAAA GGGATCCTAG
      181 CAGTTCCGAT GTAAACGAAG CTCTTAAGCT GATAGTGGAG GCCATTGAAG CAGCAGTCGA
      241 CGCGTTAGAG GCGGCTGAGA GAACGGGGGA CCCTGAAGTG AGGGAATTAG CGCGTGAACT
      301 AGTCAGGTTG GCTGTAGAAG CCGCTGAGGA AGTACAAAGA AACCCGTCCA GTTCAGATGT
      361 TAATGAAGCG CTGCATTCTA TTGTTTATGC GATTGAGGCG GCGATTTTCG CTCTTGAAGC
      421 GGCTGAGCGT ACCGGTGATC CTGAGGTAAG AGAACTAGCA AGAGAGTTAG TCAGACTTGC
      481 CGTTGAAGCT GCTGAAGAAG TTCAACGTAA TCCATCTTCA AGGAACGTAG AACATGCCTT
      541 GATGAGAATA GTGCTAGCCA TATACCTGGC GGAAGAAAAC CTACGTGAGG CCGAGGAGTC
      601 TGGTGATCCA GAAAAGAGAG AAAAGGCAAG GGAGAGAGTC AGGGAGGCTG TGGAAAGGGC
      661 AGAGGAGGTC CAGCGTGATC CAAGTGGGTG GCTGAATCAT ggATCCtGAG ACCagaccaa
      721 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      781 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      841 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      901 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      961 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     1021 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
     1081 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     1141 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     1201 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     1261 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     1321 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     1381 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1441 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1501 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1561 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1801 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2341 tgtggataac cgtag
//