LOCUS       pWN062-LexA-NES         2313 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     Imported using the Genbank importer. File name: pWN062-LexA-NES.gb
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    10..13
                     /label="type_3_fw"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /locus_tag="type_3_fw"
                     /label="type_3_fw"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    14..631
                     /label="LexA_IDTyeastcodonopt_from_addgene_plasmid_123052"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="LexA_IDTyeastcodonopt_from_addgene_plasmid_123052"
                     /label="LexA_IDTyeastcodonopt_from_addgene_plasmid_123052"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    61..61
                     /label="C to T to remove BsaI site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="C to T to remove BsaI site"
                     /label="C to T to remove BsaI site"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    628..628
                     /label="T to A to remove BsaI site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="T to A to remove BsaI site"
                     /label="T to A to remove BsaI site"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    632..658
                     /label="nuclear_export_signal_IDTyeastcodonoptimized_from_a ddgeneplasmid_42608"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="nuclear_export_signal_IDTyeastcodonoptimized_from_a ddgeneplasmid_42608"
                     /label="nuclear_export_signal_IDTyeastcodonoptimized_from_a ddgeneplasmid_42608"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    661..664
                     /label="3a rv"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="3a rv"
                     /label="3a rv"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(666..671)
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="BsaI(1)"
                     /label="BsaI(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     Antibiotic r... complement(674..782)
                     /label="CmR Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(783..1442)
                     /label="CmR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(783..1442)
                     /label="CamR"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
                     /locus_tag="CamR"
                     /label="CamR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
     Antibiotic r... complement(1443..1547)
                     /label="CmR Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(1548..2311)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 TCGGTCTCAT ATGAAGGCAC TAACGGCGCG TCAACAGGAA GTATTTGATC TAATCAGAGA
       61 TCACATTTCT CAGACGGGCA TGCCACCTAC GAGAGCGGAA ATTGCGCAGA GGCTGGGGTT
      121 TCGTTCACCA AACGCGGCCG AGGAACACCT GAAGGCTCTA GCAAGAAAAG GTGTCATTGA
      181 GATAGTCTCC GGTGCCTCCA GGGGAATCAG GCTTCTACAA GAGGAGGAGG AAGGACTGCC
      241 ACTTGTAGGA AGGGTCGCTG CAGGGGAGCC TCTATTGGCG CAGCAGCATA TCGAAGGTCA
      301 TTATCAGGTC GATCCATCTT TGTTCAAGCC CAATGCCGAT TTCTTACTTC GTGTGAGTGG
      361 AATGAGTATG AAAGATATAG GCATTATGGA TGGGGATCTA CTTGCTGTTC ATAAAACACA
      421 AGATGTGAGA AATGGTCAGG TAGTTGTAGC AAGGATCGAT GACGAAGTCA CAGTTAAAAG
      481 GCTAAAGAAG CAAGGCAATA AAGTAGAGTT ATTACCCGAG AACTCAGAAT TTAAGCCTAT
      541 TGTGGTCGAC CTACGTCAAC AGAGCTTCAC CATAGAAGGT CTGGCCGTCG GTGTTATCCG
      601 TAACGGTGAT TGGCTGGGGT CAGGGTCACC TCTGCCTCCG TTAGAAAGAC TAACGCTGGG
      661 TTCTTGAGAC Cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      721 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      781 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
      841 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
      901 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
      961 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
     1021 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1081 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1141 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1201 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1261 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1321 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1381 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     1441 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1501 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
     1561 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1681 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1741 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1801 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1861 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1921 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1981 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2041 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2101 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2161 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2221 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     2281 tgcgttatcc cctgattctg tggataaccg tag
//