LOCUS       pRO275-NLS-VP48-1XOaf1  1896 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            mNeonGreen_in_yeast_moclo_vector.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    10..13
                     /label="type_3_fw"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    14..34
                     /label="SV40_NLS_IDT_yeast_optimized_source_addgene104906"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    35..151
                     /label="VP48_from_addgene_plasmid_48236_IDTyeastcodonoptimi zed"
                     /ApEinfo_revcolor="#e24735"
                     /ApEinfo_fwdcolor="#e24735"
     misc_feature    85..85
                     /label="t->A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    92..112
                     /label="repeat_region_ofVP48"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    94..94
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    97..97
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    100..100
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    103..103
                     /label="a->G"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    112..112
                     /label="a->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    131..151
                     /label="repeat_Region_of_VP48"
                     /ApEinfo_revcolor="#ff4e69"
                     /ApEinfo_fwdcolor="#ff4e69"
     misc_feature    145..145
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    152..160
                     /label="gly-ser-gly"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    161..241
                     /label="Native_Oaf1seq_AD_1021_1047"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    244..247
                     /label="3a rv"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(249..254)
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Antibiotic r... complement(257..365)
                     /label="CmR Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(366..1025)
                     /label="CmR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(1026..1130)
                     /label="CmR Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(1131..1894)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 TCGGTCTCAT ATGCCCAAGA AGAAGCGTAA AGTGCCGGCG GATGCTCTAG ACGATTTCGA
       61 TCTGGATATG CTACCAGCCG ATGCACTTGA CGATTTCGAC TTAGACATGC TTCCGGCCGA
      121 CGCTCTAGAT GACTTCGACT TAGATATGCT CGGCTCAGGT GCGAATAATA CTCCTTTTCC
      181 AGGTTATTTT GGAGGTTTGG ATTTATTTGA TTATGACTTT TTGTTTGGCA ATGACTTTGC
      241 TGGTTCTTGA GACCagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      301 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
      361 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      421 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
      481 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
      541 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac
      601 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
      661 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
      721 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
      781 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
      841 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
      901 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
      961 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1021 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1081 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa
     1141 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1201 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1261 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1321 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1381 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1441 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1501 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1561 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1621 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1681 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1741 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1801 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1861 tcctgcgtta tcccctgatt ctgtggataa ccgtag
//