LOCUS       pRO276-NLS-VP48-2XOaf1  1986 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            mNeonGreen_in_yeast_moclo_vector.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    10..13
                     /label="type_3_fw"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    14..34
                     /label="SV40_NLS_IDT_yeast_optimized_source_addgene104906"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    35..151
                     /label="VP48_from_addgene_plasmid_48236_IDTyeastcodonoptimi zed"
                     /ApEinfo_revcolor="#e24735"
                     /ApEinfo_fwdcolor="#e24735"
     misc_feature    85..85
                     /label="t->A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    92..112
                     /label="repeat_region_ofVP48"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    94..94
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    97..97
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    100..100
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    103..103
                     /label="a->G"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    112..112
                     /label="a->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    131..151
                     /label="repeat_Region_of_VP48"
                     /ApEinfo_revcolor="#ff4e69"
                     /ApEinfo_fwdcolor="#ff4e69"
     misc_feature    145..145
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    152..160
                     /label="gly-ser-gly"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    161..241
                     /label="Oaf1_AD_1021-1047"
                     /ApEinfo_revcolor="#86a74b"
                     /ApEinfo_fwdcolor="#86a74b"
     misc_feature    211..211
                     /label="t->C"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    214..214
                     /label="t->C"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    217..217
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    220..220
                     /label="t->C"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    242..250
                     /label="gly-ser-gly"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    251..331
                     /label="Native_Oaf1seq_AD_1021_1047"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    334..337
                     /label="3a rv"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(339..344)
                     /label="BsaI"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Antibiotic r... complement(347..455)
                     /label="CmR Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(456..1115)
                     /label="CmR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(1116..1220)
                     /label="CmR Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(1221..1984)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 TCGGTCTCAT ATGCCCAAGA AGAAGCGTAA AGTGCCGGCG GATGCTCTAG ACGATTTCGA
       61 TCTGGATATG CTACCAGCCG ATGCACTTGA CGATTTCGAC TTAGACATGC TTCCGGCCGA
      121 CGCTCTAGAT GACTTCGACT TAGATATGCT CGGTTCCGGC GCGAACAATA CACCATTTCC
      181 GGGATATTTT GGGGGCCTTG ATTTATTCGA CTACGACTTC CTTTTCGGCA ACGACTTTGC
      241 TGGCTCAGGT GCGAATAATA CTCCTTTTCC AGGTTATTTT GGAGGTTTGG ATTTATTTGA
      301 TTATGACTTT TTGTTTGGCA ATGACTTTGC TGGTTCTTGA GACCagacca ataaaaaacg
      361 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
      421 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
      481 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
      541 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
      601 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
      661 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
      721 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      781 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      841 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      901 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      961 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1021 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1081 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1141 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1201 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1981 ccgtag
//