LOCUS       pWN102-NLS-VP48-2XOaf1  1984 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     Imported using the Genbank importer. File name:
            pWN102-NLS-VP48(2XOaf1).gb ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label="BsaI"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    10..13
                     /label="4a fwd overhang"
                     /ApEinfo_revcolor="#ff51d7"
                     /ApEinfo_fwdcolor="#ff51d7"
                     /locus_tag="4a fwd overhang"
                     /label="4a fwd overhang"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    14..34
                     /label="SV40_NLS_IDT_yeast_optimized_source_addgene104906"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="SV40_NLS_IDT_yeast_optimized_source_addgene104906"
                     /label="SV40_NLS_IDT_yeast_optimized_source_addgene104906"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             14..331
                     /label="NLS-VP48-2XOaf1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="PKKKRKVPADALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLGSGANNTPFPGYFGGLDLFDYDFLFGNDFAGSGANNTPFPGYFGGLDLFDYDFLFGNDFA"
     misc_feature    35..151
                     /label="VP48_from_addgene_plasmid_48236_IDTyeastcodonoptimi zed"
                     /ApEinfo_revcolor="#e24735"
                     /ApEinfo_fwdcolor="#e24735"
                     /locus_tag="VP48_from_addgene_plasmid_48236_IDTyeastcodonoptimi zed"
                     /label="VP48_from_addgene_plasmid_48236_IDTyeastcodonoptimi zed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    85..85
                     /label="t->A"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="t->A"
                     /label="t->A"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    92..112
                     /label="repeat_region_ofVP48"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="repeat_region_ofVP48"
                     /label="repeat_region_ofVP48"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    94..94
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="c->T(4)"
                     /label="c->T(4)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    97..97
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="c->T"
                     /label="c->T"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    100..100
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="c->T(3)"
                     /label="c->T(3)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    103..103
                     /label="a->G"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="a->G"
                     /label="a->G"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    112..112
                     /label="a->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="a->T"
                     /label="a->T"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    131..151
                     /label="repeat_Region_of_VP48"
                     /ApEinfo_revcolor="#ff4e69"
                     /ApEinfo_fwdcolor="#ff4e69"
                     /locus_tag="repeat_Region_of_VP48"
                     /label="repeat_Region_of_VP48"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    145..145
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="c->T(1)"
                     /label="c->T(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    152..160
                     /label="gly-ser-gly"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="gly-ser-gly"
                     /label="gly-ser-gly"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    161..241
                     /label="Oaf1_AD_1021-1047"
                     /ApEinfo_revcolor="#86a74b"
                     /ApEinfo_fwdcolor="#86a74b"
                     /locus_tag="Oaf1_AD_1021-1047"
                     /label="Oaf1_AD_1021-1047"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    211..211
                     /label="t->C"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="t->C"
                     /label="t->C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    214..214
                     /label="t->C"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="t->C(1)"
                     /label="t->C(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    217..217
                     /label="c->T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="c->T(2)"
                     /label="c->T(2)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    220..220
                     /label="t->C"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="t->C(2)"
                     /label="t->C(2)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    242..250
                     /label="gly-ser-gly"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="gly-ser-gly(1)"
                     /label="gly-ser-gly(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    251..331
                     /label="Native_Oaf1seq_AD_1021_1047"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="Native_Oaf1seq_AD_1021_1047"
                     /label="Native_Oaf1seq_AD_1021_1047"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    332..335
                     /label="4a rv overhang"
                     /ApEinfo_revcolor="#ff3dce"
                     /ApEinfo_fwdcolor="#ff3dce"
                     /locus_tag="4a rv overhang"
                     /label="4a rv overhang"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     Antibiotic r... complement(345..453)
                     /label="CmR Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... complement(454..1113)
                     /label="CmR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(454..1113)
                     /label="CamR"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_fwdcolor="#0000ff"
                     /locus_tag="CamR"
                     /label="CamR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
     Antibiotic r... complement(1114..1218)
                     /label="CmR Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Origin of Re... 1219..1982
                     /label="ColE1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 tcggtctcaA TCCCCCAAGA AGAAGCGTAA AGTGCCGGCG GATGCTCTAG ACGATTTCGA
       61 TCTGGATATG CTACCAGCCG ATGCACTTGA CGATTTCGAC TTAGACATGC TTCCGGCCGA
      121 CGCTCTAGAT GACTTCGACT TAGATATGCT CGGTTCCGGC GCGAACAATA CACCATTTCC
      181 GGGATATTTT GGGGGCCTTG ATTTATTCGA CTACGACTTC CTTTTCGGCA ACGACTTTGC
      241 TGGCTCAGGT GCGAATAATA CTCCTTTTCC AGGTTATTTT GGAGGTTTGG ATTTATTTGA
      301 TTATGACTTT TTGTTTGGCA ATGACTTTGC TTGGCtgaga ccagaccaat aaaaaacgcc
      361 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      421 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
      481 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      541 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      601 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      661 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
      721 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      781 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
      841 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
      901 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
      961 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1021 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1081 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1141 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1201 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1441 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1681 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1981 gtag
//