LOCUS       pAL002-VP48-2XOaf1      1966 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pal002-vp482xoaf.gb
            Imported using the Genbank importer. File name:
            vo2_vp48partplasmid_fixedfinal.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     Antibiotic r... 367..471
                     /label="CmR Promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     Antibiotic r... 472..1131
                     /label="CmR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             472..1131
                     /label="CmR"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_fwdcolor="#e9d024"
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /locus_tag="CmR"
                     /label="CmR"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
     Antibiotic r... 1132..1240
                     /label="CmR Terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1254..1370
                     /label="VP48_from_addgene_plasmid_48236_IDTyeastcodonoptimi zed"
                     /ApEinfo_revcolor="#e24735"
                     /ApEinfo_fwdcolor="#e24735"
     CDS             1254..1553
                     /label="Translation 1254-1553"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /locus_tag="Translation 1254-1553"
                     /label="Translation 1254-1553"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
                     /translation="PADALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLGSGANNTPFPGYFGGLDLFDYDFLFGNDFAGSGANNTPFPGYFGGLDLFDYDFLFGNDFA*"
     CDS             1254..1553
                     /label="VO2_VP48"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="VO2_VP48"
                     /label="VO2_VP48"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
                     /translation="PADALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLGSGANNTPFPGYFGGLDLFDYDFLFGNDFAGSGANNTPFPGYFGGLDLFDYDFLFGNDFA*"
     misc_feature    1380..1460
                     /label="Oaf1_AD_1021-1047"
                     /ApEinfo_revcolor="#86a74b"
                     /ApEinfo_fwdcolor="#86a74b"
     misc_feature    1470..1550
                     /label="Native_Oaf1seq_AD_1021_1047"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     Origin of Re... 1569..366
                     /label="ColE1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
       61 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      121 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      241 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      301 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      361 tcatgattga tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
      421 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
      481 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      541 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      601 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      661 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
      721 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
      781 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
      841 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
      901 ttcgtttcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
      961 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     1021 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     1081 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgatatc
     1141 gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     1201 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt ctggtctcaA TCCccggcgg
     1261 atgctctaga cgatttcgat ctggatatgc taccagccga tgcacttgac gatttcgact
     1321 tagacatgct tccggccgac gctctagatg acttcgactt agatatgctc ggttccggcg
     1381 cgaacaatac accatttccg ggatattttg ggggccttga tttattcgac tacgacttcc
     1441 ttttcggcaa cgactttgct ggctcaggtg cgaataatac tccttttcca ggttattttg
     1501 gaggtttgga tttatttgat tatgactttt tgtttggcaa tgactttgct taaTGGCtga
     1561 gaccgactac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1621 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1681 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1741 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1801 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1861 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1921 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggta
//