LOCUS       pRO263-pRAD27-mNeonGree 3889 bp ds-DNA     circular     06-DEC-2024
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     Imported using the Genbank importer. File name: plasmids/pYTK095.gb
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    28..170
                     /label="ConS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    171..176
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    178..181
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    178..202
                     /label="ConS scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        207..900
                     /label="ScRAD27 Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    910..1614
                     /label="yo_mNeonGreen"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      1630..1854
                     /label="ScENO2 Terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    complement(1859..1862)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    complement(1864..1869)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1870..2012
                     /label="ConRE"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      complement(2040..2136)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(2137..2994)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
     promoter        complement(2995..3125)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(3126..3889)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagatgg tagagccaca aacagccggt acaagcaacg
       61 atctccagga ccatctgaat catgcgcgga tgacacgaac tcacgacggc gatcacagac
      121 attaacccac agtacagaca ctgcgacaac gtggcaattc gtcgcaatac cgtctcactg
      181 aactggccga taattgcaga cgaacgcctt gtgaaattgc aaatatggtg atttgaaacg
      241 tttcctagtg cagcaggatc acagataacg tgtaaagggc ttagcagttg ataatcctct
      301 ctagttaaga cctaaacaaa atgctgtcac taaccgtagt attaaatgac acactttggt
      361 gactttcgtt aatggggatg tggtagtggc cattgccaat aaacaaaaag aacagggaaa
      421 gaagtagaaa gtgatataag tttgcttgcc acttttcgtt tttcacgaaa aaaacaggcg
      481 aaaaaaaatg ctagacaagt acccggctga atcacacctc gttaacagtg actttcggtg
      541 acagataccc gattgggcac ccggctggta agttatgata gaaagccaac gctgtactat
      601 tggcttagct atggcaatat tttgattatc agctagtttt attaacgtta taattagtgt
      661 aaccagtttt tcatctattt catttatttc atttatttac tttaattgca gatcccccta
      721 acgcgtttaa agcttttatt cactagctta tgtatttttt ataggaaacg cgacgcgtaa
      781 catcgcgcaa atgaaggttt tgatgtatta taatgaggta ttcttcctta tatacatcga
      841 tgaaaagcgt tgacagcata cattggaaag aaataggaaa cggacaccgg aagaaaaaat
      901 agatctatgG TTTCAAAAGG GGAGGAGGAT AATATGGCGA GTCTTCCTGC CACGCATGAG
      961 CTGCATATCT TCGGATCTAT TAATGGTGTC GACTTTGATA TGGTAGGACA AGGAACCGGG
     1021 AACCCCAATG ACGGGTATGA AGAGCTAAAT CTAAAGAGTA CAAAAGGAGA TTTACAGTTT
     1081 TCTCCTTGGA TACTTGTTCC CCACATTGGA TATGGATTTC ATCAATATCT TCCTTATCCA
     1141 GACGGAATGA GCCCGTTCCA GGCCGCTATG GTTGACGGCT CCGGGTATCA AGTACACCGT
     1201 ACCATGCAGT TTGAGGATGG GGCATCCCTG ACCGTAAATT ACAGGTACAC CTACGAGGGC
     1261 TCACACATAA AGGGTGAGGC GCAGGTAAAG GGTACTGGAT TTCCTGCGGA CGGCCCAGTA
     1321 ATGACAAATT CACTTACAGC AGCGGATTGG TGCAGGAGCA AAAAGACCTA TCCCAATGAC
     1381 AAAACTATCA TAAGTACCTT TAAATGGTCC TATACGACAG GTAACGGGAA GAGATATAGG
     1441 TCAACGGCAA GGACAACGTA TACTTTCGCG AAGCCAATGG CAGCAAACTA TCTGAAGAAT
     1501 CAACCTATGT ACGTGTTCAG AAAGACCGAG TTGAAACATT CCAAGACAGA GTTAAATTTC
     1561 AAAGAATGGC AAAAGGCTTT TACTGACGTA ATGGGGATGG ATGAATTGTA TAAGggatcc
     1621 taactcgaga gtgcttttaa ctaagaatta ttagtctttt ctgcttattt tttcatcata
     1681 gtttagaaca ctttatatta acgaatagtt tatgaatcta tttaggttta aaaattgata
     1741 cagttttata agttactttt tcaaagactc gtgctgtcta ttgcataatg cactggaagg
     1801 ggaaaaaaaa ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa aaatgctgag
     1861 catgagacgg aaatctgctc gtcagtggtg ctcacactga cgaatcatgt acagatcata
     1921 ccgatgactg cctggcgact cacaactaag caagacagcc ggaaccagcg ccggcgaaca
     1981 ccactgcata tatggcatat cacaacagtc caactagtgc actgcagtac agcggccgcg
     2041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc
     2281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3121 acctgtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3181 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3241 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3301 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3361 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3421 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3481 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3541 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3601 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3661 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3721 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3781 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3841 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgt
//