LOCUS       pRO229-pRPL18B-rtTA-v10 3956 bp ds-DNA     circular     07-DEC-2024
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     Imported using the Genbank importer. File name: plasmids/pYTK095.gb
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     protein_bind    27..30
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    46..188
                     /label="ConS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    189..194
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    196..199
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    196..220
                     /label="ConS scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    221..224
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        225..924
                     /label="ScRPL18B Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    930..933
                     /label="type_3_fw"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    934..1674
                     /label="TET3G_yo"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1677..1680)
                     /label="type_3_rv"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      1690..1914
                     /label="ScADH1 Terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    complement(1915..1918)
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    complement(1919..1922)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    complement(1924..1929)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1930..2072
                     /label="Con1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    complement(2088..2091)
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    complement(2093..2098)
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      complement(2107..2203)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(2204..3061)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
     promoter        complement(3062..3192)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(3193..3956)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 gcggccgccc tgaattcgca tctagaccct gaattcgcat ctagatggta gagccacaaa
       61 cagccggtac aagcaacgat ctccaggacc atctgaatca tgcgcggatg acacgaactc
      121 acgacggcga tcacagacat taacccacag tacagacact gcgacaacgt ggcaattcgt
      181 cgcaataccg tctcactgaa ctggccgata attgcagacg aacgaagagg atgtccaata
      241 ttttttttaa ggaataagga tacttcaaga ctagattccc ccctgcattc ccatcagaac
      301 cgtaaacctt ggcgctttcc ttgggaagta ttcaagaagt gccttgtccg gtttctgtgg
      361 ctcacaaacc agcgcgcccg atatggcttt cttttcactt atgaatgtac cagtacggga
      421 caattagaac gctcctgtaa caatctcttt gcaaatgtgg ggttacattc taaccatgtc
      481 acactgctga cgaaattcaa agtaaaaaaa aatgggacca cgtcttgaga acgatagatt
      541 ttctttattt tacattgaac agtcgttgtc tcagcgcgct ttatgttttc attcatactt
      601 catattataa aataacaaaa gaagaatttc atattcacgc ccaagaaatc aggctgcttt
      661 ccaaatgcaa ttgacacttc attagccatc acacaaaact ctttcttgct ggagcttctt
      721 ttaaaaaaga cctcagtaca ccaaacacgt tacccgacct cgttatttta cgacaactat
      781 gataaaattc tgaagaaaaa ataaaaaaat tttcatactt cttgctttta tttaaaccat
      841 tgaatgattt cttttgaaca aaactacctg tttcaccaaa ggaaatagaa agaaaaaatc
      901 aattagaaga aaacaaaaaa caaaagatcT ATGTCCCGTC TTGATAAGAG TAAGGTTATC
      961 AACTCAGCCC TAGAGCTGCT GAACGGCGTC GGAATCGAGG GGCTTACTAC CAGAAAACTA
     1021 GCACAGAAAC TGGGTGTAGA GCAGCCCACG CTTTACTGGC ATGTTAAAAA TAAGAGAGCA
     1081 CTATTAGACG CGTTGCCGAT CGAAATGTTG GATAGGCATC ACACACACTC ATGCCCTTTA
     1141 GAAGGAGAAA GTTGGCAGGA TTTTCTGAGA AATAATGCGA AGTCTTATCG TTGTGCGTTA
     1201 CTTTCCCACA GGGACGGTGC TAAAGTACAT TTGGGAACTA GACCAACTGA AAAACAATAT
     1261 GAGACATTGG AGAACCAACT GGCCTTCTTA TGCCAACAGG GTTTCAGTCT TGAGAACGCC
     1321 CTTTACGCCC TTTCAGCGGT TGGGCACTTC ACTCTTGGTT GCGTCTTGGA AGAGCAGGAG
     1381 CATCAAGTAG CAAAAGAGGA GCGTGAAACC CCCACAACAG ACTCCATGCC CCCTCTATTG
     1441 AAGCAAGCTA TCGAATTATT CGACCGTCAA GGAGCGGAAC CAGCATTTTT ATTTGGCTTA
     1501 GAATTGATTA TTTGTGGGTT AGAAAAACAA CTAAAGTGTG AGAGCGGTGG ACCGACAGAC
     1561 GCTCTTGATG ATTTTGACTT GGATATGTTA CCCGCCGACG CTTTGGACGA CTTCGATTTG
     1621 GATATGCTGC CTGCGGATGC ACTAGATGAT TTCGATCTGG ACATGTTACC GGGTGGATCC
     1681 taactcgagg cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
     1741 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
     1801 agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt
     1861 gaccacacct ctaccggcat gccgagcaaa tgcctgcaaa tcgctcccca tttcgctgcc
     1921 aatgagacga cggggtcatc acggctcatc atgcgccaaa caaatgtgtg caatacacgc
     1981 tcggatgact gcatgatgac cgcactgact ggggacagca gatccaccta agcctgtgag
     2041 agaagcagac acccgacaga tcaaggcagt taactagtgc actgcagtac atgagaccgc
     2101 ggccgcgatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2161 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2221 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2281 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2341 gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2401 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2461 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2521 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2581 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2641 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2701 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2761 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2821 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2881 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2941 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3001 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3061 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3121 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3181 aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3241 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3301 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3361 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3421 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3481 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3541 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3601 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3661 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3721 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3781 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3841 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3901 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgt
//