LOCUS       pRO230-pRPL18B-rtTA3-tA 3917 bp ds-DNA     circular     07-DEC-2024
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     Imported using the Genbank importer. File name: plasmids/pYTK095.gb
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     protein_bind    27..30
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    46..188
                     /label="ConS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    189..194
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    196..199
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    196..220
                     /label="ConS scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    221..224
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     promoter        225..924
                     /label="ScRPL18B Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    930..933
                     /label="type_3_fw"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    934..1635
                     /label="rtTA3_yeastcodonoptimized_IDT_from_addgeneplasmid60431"
                     /ApEinfo_revcolor="#9f7cff"
                     /ApEinfo_fwdcolor="#9f7cff"
     misc_feature    complement(1638..1641)
                     /label="type_3_rv"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      1651..1875
                     /label="ScADH1 Terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    complement(1876..1879)
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    complement(1880..1883)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    complement(1885..1890)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1891..2033
                     /label="Con1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    complement(2049..2052)
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    complement(2054..2059)
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      complement(2068..2164)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(2165..3022)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
     promoter        complement(3023..3153)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(3154..3917)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 gcggccgccc tgaattcgca tctagaccct gaattcgcat ctagatggta gagccacaaa
       61 cagccggtac aagcaacgat ctccaggacc atctgaatca tgcgcggatg acacgaactc
      121 acgacggcga tcacagacat taacccacag tacagacact gcgacaacgt ggcaattcgt
      181 cgcaataccg tctcactgaa ctggccgata attgcagacg aacgaagagg atgtccaata
      241 ttttttttaa ggaataagga tacttcaaga ctagattccc ccctgcattc ccatcagaac
      301 cgtaaacctt ggcgctttcc ttgggaagta ttcaagaagt gccttgtccg gtttctgtgg
      361 ctcacaaacc agcgcgcccg atatggcttt cttttcactt atgaatgtac cagtacggga
      421 caattagaac gctcctgtaa caatctcttt gcaaatgtgg ggttacattc taaccatgtc
      481 acactgctga cgaaattcaa agtaaaaaaa aatgggacca cgtcttgaga acgatagatt
      541 ttctttattt tacattgaac agtcgttgtc tcagcgcgct ttatgttttc attcatactt
      601 catattataa aataacaaaa gaagaatttc atattcacgc ccaagaaatc aggctgcttt
      661 ccaaatgcaa ttgacacttc attagccatc acacaaaact ctttcttgct ggagcttctt
      721 ttaaaaaaga cctcagtaca ccaaacacgt tacccgacct cgttatttta cgacaactat
      781 gataaaattc tgaagaaaaa ataaaaaaat tttcatactt cttgctttta tttaaaccat
      841 tgaatgattt cttttgaaca aaactacctg tttcaccaaa ggaaatagaa agaaaaaatc
      901 aattagaaga aaacaaaaaa caaaagatcT ATGAGTCGTT TAGATAAATC CAAGGTTATA
      961 AACGGCGCAC TAGAACTGCT TAATGGCGTT GGTATCGAAG GTCTGACTAC GAGGAAGTTA
     1021 GCACAGAAAC TTGGGGTCGA GCAGCCCACG CTGTATTGGC ACGTAAAGAA CAAGAGAGCG
     1081 CTATTAGATG CCCTACCAAT AGAGATGTTA GATAGACACC ATACTCATTT TTGCCCGCTT
     1141 GAGGGGGAAA GCTGGCAAGA CTTTTTAAGA AATAATGCAA AGAGTTACAG GTGCGCTCTT
     1201 CTTTCACATA GGGATGGGGC AAAGGTACAC TTAGGTACGA GGCCAACCGA GAAACAGTAT
     1261 GAAACACTAG AAAACCAACT TGCTTTTTTA TGCCAACAGG GTTTCAGTCT TGAGAATGCA
     1321 CTTTATGCCC TTAGCGCAGT CGGGCACTTT ACGTTGGGTT GCGTCCTAGA GGAGCAGGAA
     1381 CATCAAGTCG CAAAAGAGGA ACGTGAGACA CCGACTACTG ATAGTATGCC CCCGTTATTA
     1441 CGTCAGGCAA TAGAACTATT TGACCGTCAA GGGGCGGAAC CGGCTTTTCT TTTTGGCCTG
     1501 GAGCTAATTA TTTGTGGGCT GGAGAAGCAA CTGAAGTGCG AAAGTGGTGG GCCAACTGAC
     1561 GCGCTAGATG ATTTCGATCT TGATATGTTA CCTGCAGATG CACTAGATGA CTTTGATTTA
     1621 GACATGTTGC CTGGAGGATC Ctaactcgag gcgaatttct tatgatttat gatttttatt
     1681 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     1741 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     1801 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccgagcaa atgcctgcaa
     1861 atcgctcccc atttcgctgc caatgagacg acggggtcat cacggctcat catgcgccaa
     1921 acaaatgtgt gcaatacacg ctcggatgac tgcatgatga ccgcactgac tggggacagc
     1981 agatccacct aagcctgtga gagaagcaga cacccgacag atcaaggcag ttaactagtg
     2041 cactgcagta catgagaccg cggccgcgat tatcaaaaag gatcttcacc tagatccttt
     2101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2281 ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta tcagcaataa
     2341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3001 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3061 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3121 ttccgcgcac atttccccga aaagtgccac ctgtcatgac caaaatccct taacgtgagt
     3181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3841 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3901 attctgtgga taaccgt
//