LOCUS       pRO204-pTDH3-NLS-VP48-A 4214 bp ds-DNA     circular     07-DEC-2024
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     Imported using the Genbank importer. File name: plasmids/pYTK095.gb
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    28..170
                     /label="Con1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    171..176
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    178..181
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    178..202
                     /label="Con1 scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        207..886
                     /label="ScTDH3 Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    896..916
                     /label="SV40_NLS_IDT_yeast_optimized_source_addgene104906"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    917..1033
                     /label="VP48_from_addgene_plasmid_48236_IDTyeastcodonoptimized"
                     /ApEinfo_revcolor="#e24735"
                     /ApEinfo_fwdcolor="#e24735"
     misc_feature    1040..1939
                     /label="ABI_from_addgene_plasmid84243_IDTyeastcodonoptimized"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1105..1105
                     /label="remove_bsmbi_site"
                     /ApEinfo_revcolor="#575566"
                     /ApEinfo_fwdcolor="#575566"
     misc_feature    1108..1108
                     /label="A->G to remove bsaI site"
                     /ApEinfo_revcolor="#575566"
                     /ApEinfo_fwdcolor="#575566"
     terminator      1955..2179
                     /label="ScENO1 Terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     protein_bind    complement(2184..2187)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    complement(2189..2194)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2195..2337
                     /label="Con2"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     terminator      complement(2365..2461)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(2462..3319)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
     promoter        complement(3320..3450)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(3451..4214)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
ORIGIN
        1 gcggccgccc ctgaattcgc atctagaacg gggtcatcac ggctcatcat gcgccaaaca
       61 aatgtgtgca atacacgctc ggatgactgc atgatgaccg cactgactgg ggacagcaga
      121 tccacctaag cctgtgagag aagcagacac ccgacagatc aaggcagtta cgtctcacca
      181 aaccagatgt caacacagct acaacgcagt tcgagtttat cattatcaat actgccattt
      241 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
      301 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
      361 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
      421 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
      481 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
      541 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
      601 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
      661 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
      721 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
      781 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta
      841 aaacaccaag aacttagttt cgaataaaca cacataaaca aacaaaagat cTATGCCCAA
      901 GAAGAAGCGT AAAGTGCCGG CGGATGCTCT AGACGATTTC GATCTGGATA TGCTACCAGC
      961 CGATGCTCTT GACGACTTCG ACTTAGACAT GCTACCGGCC GACGCTCTAG ATGACTTCGA
     1021 CTTAGACATG CTCGGTTCTA CGAGAGTTCC TTTGTATGGC TTCACCTCAA TTTGCGGTAG
     1081 AAGACCCGAG ATGGAAGCCG CCGTATCGAC GATACCACGT TTTTTACAAT CCTCCTCCGG
     1141 CTCAATGTTA GACGGACGTT TTGATCCCCA GAGTGCAGCT CATTTCTTTG GTGTTTATGA
     1201 TGGTCACGGG GGTAGCCAGG TAGCCAACTA TTGCCGTGAA AGGATGCATT TAGCGTTAGC
     1261 TGAGGAAATC GCCAAAGAAA AGCCTATGTT GTGTGATGGA GACACCTGGT TGGAGAAGTG
     1321 GAAAAAGGCG TTATTCAACA GCTTCCTAAG GGTGGATAGT GAGATAGAGT CTGTTGCTCC
     1381 GGAGACTGTT GGCTCAACAT CCGTGGTGGC CGTGGTCTTC CCGAGCCATA TTTTTGTGGC
     1441 TAATTGTGGT GACTCTCGTG CGGTCTTGTG CAGGGGGAAG ACTGCATTAC CTTTAAGCGT
     1501 CGATCATAAG CCGGACAGGG AGGACGAAGC AGCGAGGATA GAGGCCGCCG GTGGAAAGGT
     1561 AATACAATGG AATGGTGCTA GGGTATTCGG CGTACTGGCA ATGAGTAGAT CCATTGGTGA
     1621 TAGATACTTG AAGCCGAGCA TAATCCCTGA CCCTGAAGTA ACAGCGGTTA AAAGGGTGAA
     1681 GGAAGACGAT TGCCTTATCT TGGCTTCTGA CGGAGTGTGG GATGTCATGA CTGACGAGGA
     1741 AGCCTGCGAA ATGGCAAGGA AAAGAATACT ACTTTGGCAT AAGAAAAATG CGGTAGCTGG
     1801 GGACGCAAGT CTGTTAGCAG ATGAAAGAAG AAAAGAAGGC AAGGACCCCG CCGCAATGTC
     1861 AGCCGCGGAG TACCTGTCTA AACTTGCTAT TCAGAGGGGG AGTAAAGATA ACATATCCGT
     1921 GGTCGTGGTT GATCTAAAGG GATCCtaact cgagagcttt tgattaagcc ttctagtcca
     1981 aaaaacacgt ttttttgtca tttatttcat tttcttagaa tagtttagtt tattcatttt
     2041 atagtcacga atgttttatg attctatata gggttgcaaa caagcatttt tcattttatg
     2101 ttaaaacaat ttcaggttta ccttttattc tgcttgtggt gacgcgtgta tccgcccgct
     2161 cttttggtca cccatgtatg ctggatgtga gacgataaat cgcagccaag tgagtgaata
     2221 gatgacgcac cacggtcaga cacggccaca tcgtatctca caggagcaag cgcgatagga
     2281 gcactcacac atagtacggt gatccgctga ctcctttgcc caaataagac gtgagccact
     2341 agtgcactgc agtacagcgg ccgcgattat caaaaaggat cttcacctag atccttttaa
     2401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2581 gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca gcaataaacc
     2641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3421 cgcgcacatt tccccgaaaa gtgccacctg tcatgaccaa aatcccttaa cgtgagtttt
     3481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3601 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3661 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3721 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3781 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3841 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3901 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3961 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4021 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4081 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4141 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4201 ctgtggataa ccgt
//