LOCUS       pAL008-pTDH3-LexA-NLS-N 4992 bp ds-DNA     circular     18-JAN-2025
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
COMMENT     Imported using the Genbank importer. File name:
            pAL008-pTDH3-LexA-NS3-VP48(2XOaf1)-tADH1.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    716..858
                     /label="ConS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="ConS"
                     /label="ConS"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     protein_bind    859..864
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="BsmBI"
                     /label="BsmBI"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     protein_bind    866..869
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="BsmBI(1)"
                     /label="BsmBI(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    866..890
                     /label="ConS scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="ConS scar"
                     /label="ConS scar"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     promoter        895..1574
                     /label="ScTDH3 Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /locus_tag="ScTDH3 Promoter"
                     /label="ScTDH3 Promoter"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    1584..2201
                     /label="LexA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2202..2222
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2250..2279
                     /label="T7 tag"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /locus_tag="T7 tag"
                     /label="T7 tag"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2289..2318
                     /label="NS5A/5B cutsite (CC)"
                     /ApEinfo_revcolor="#746fc9"
                     /ApEinfo_fwdcolor="#746fc9"
                     /locus_tag="NS5A?5B cutsite (CC)"
                     /label="NS5A?5B cutsite (CC)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2340..2357
                     /label="AU1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /locus_tag="AU1"
                     /label="AU1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2373..2411
                     /label="NS4A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="NS4A"
                     /label="NS4A"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2424..2990
                     /label="NS3"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="NS3"
                     /label="NS3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2531..2531
                     /label="modified C->A to remove bsmbi cutsite"
                     /ApEinfo_revcolor="#ff4e69"
                     /ApEinfo_fwdcolor="#ff4e69"
                     /locus_tag="modified C->A to remove bsmbi cutsite"
                     /label="modified C->A to remove bsmbi cutsite"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2954..2954
                     /label="C->A to remove bsaI cutsite"
                     /ApEinfo_revcolor="#ff4e69"
                     /ApEinfo_fwdcolor="#ff4e69"
                     /locus_tag="C->A to remove bsaI cutsite"
                     /label="C->A to remove bsaI cutsite"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3042..3083
                     /label="NS4A/4B cutsite (CS)"
                     /ApEinfo_revcolor="#746fc9"
                     /ApEinfo_fwdcolor="#746fc9"
                     /locus_tag="NS4A/4B cutsite (CS)"
                     /label="NS4A/4B cutsite (CS)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3084..3110
                     /label="HA1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="HA1"
                     /label="HA1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3117..3233
                     /label="VP48_from_addgene_plasmid_48236_IDTyeastcodonoptimi zed"
                     /ApEinfo_revcolor="#e24735"
                     /ApEinfo_fwdcolor="#e24735"
     misc_feature    3243..3323
                     /label="Oaf1_AD_1021-1047"
                     /ApEinfo_revcolor="#86a74b"
                     /ApEinfo_fwdcolor="#86a74b"
     misc_feature    3333..3413
                     /label="Native_Oaf1seq_AD_1021_1047"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     terminator      3421..3645
                     /label="ScADH1 Terminator"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="ScADH1 Terminator"
                     /label="ScADH1 Terminator"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    3661..3803
                     /label="Con1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="Con1"
                     /label="Con1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     terminator      complement(3831..3927)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="AmpR Terminator"
                     /label="AmpR Terminator"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     CDS             complement(3928..4785)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
     CDS             complement(3928..4785)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9f4240"
                     /ApEinfo_fwdcolor="#9f4240"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     promoter        complement(4786..4916)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Origin of Re... complement(4917..688)
                     /label="ColE1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtgc ggccgcccct gaattcgcat ctagatggta
      721 gagccacaaa cagccggtac aagcaacgat ctccaggacc atctgaatca tgcgcggatg
      781 acacgaactc acgacggcga tcacagacat taacccacag tacagacact gcgacaacgt
      841 ggcaattcgt cgcaataccg tctcactgaa ctggccgata attgcagacg aacgcagttc
      901 gagtttatca ttatcaatac tgccatttca aagaatacgt aaataattaa tagtagtgat
      961 tttcctaact ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc
     1021 ccaaaatagg gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt
     1081 tattcctggc atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa
     1141 aaagaatccc agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc
     1201 tcttagcgca actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat
     1261 ggagtgatgc aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat
     1321 ctatctcatt ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga
     1381 aaaaaaaggt tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa
     1441 agacggtagg tattgattgt aattctgtaa atctatttct taaacttctt aaattctact
     1501 tttatagtta gtcttttttt tagttttaaa acaccaagaa cttagtttcg aataaacaca
     1561 cataaacaaa caaaagatct atgAAGGCTT TAACTGCAAG ACAACAGGAA GTTTTTGATT
     1621 TGATAAGAGA TCATATATCT CAAACTGGAA TGCCACCAAC AAGAGCTGAA ATAGCTCAAA
     1681 GATTAGGATT TAGATCTCCA AACGCTGCAG AAGAACATTT GAAAGCTTTG GCAAGGAAAG
     1741 GTGTCATCGA AATTGTCTCT GGTGCTTCAA GAGGTATTAG ACTATTACAA GAAGAGGAAG
     1801 AAGGATTGCC ATTGGTTGGT AGAGTTGCTG CTGGTGAACC ATTATTAGCA CAGCAACATA
     1861 TTGAGGGTCA TTATCAAGTA GATCCATCTT TATTCAAACC AAATGCTGAT TTTTTGTTGA
     1921 GAGTAAGTGG TATGTCTATG AAAGATATTG GTATCATGGA TGGTGACTTA TTGGCTGTTC
     1981 ATAAAACTCA AGATGTTAGA AACGGTCAAG TTGTCGTTGC CAGAATTGAT GATGAAGTCA
     2041 CTGTTAAAAG ATTGAAGAAA CAAGGTAATA AAGTGGAATT GTTACCAGAA AATTCTGAAT
     2101 TTAAGCCAAT TGTTGTTGAT TTAAGGCAAC AATCTTTTAC TATTGAAGGT TTAGCTGTCG
     2161 GTGTTATCAG AAATGGTGAT TGGTTAGGTT CAGGTTCTCC TCCAAAAAAA AAGAGAAAAG
     2221 TTGGATCTGG TTCTggggcg agcgcgggcA TGGCGTCAAT GACTGGGGGC CAGCAAATGg
     2281 ggagtacgGA GGATGTAGTT TGTTGTCATT CAATATACgg caagaagaag ggtgacatcG
     2341 ACACGTACAG ATACATAggc tctagtggga caGGCTGCGT AGTAATCGTG GGCAGAATTG
     2401 TCCTAAGCGG Atccggaacc agtGCGCCTA TCACCGCCTA CGCACAACAA ACCAGGGGAC
     2461 TATTGGGCTG TATCATAACA AGCCTAACTG GAAGAGACAA AAATCAAGTC GAGGGCGAGG
     2521 TTCAGATCGT ATCTACGGCA ACTCAGACCT TTCTTGCCAC GTGCATAAAC GGCGTGTGCT
     2581 GGGCCGTATA TCATGGGGCT GGCACAAGAA CGATAGCGAG TCCTAAGGGT CCTGTTATTC
     2641 AAATGTATAC CAACGTAGAC CAGGATCTAG TCGGCTGGCC CGCGCCCCAA GGCTCTAGGA
     2701 GTCTTACCCC TTGCACGTGC GGGTCCTCAG ACTTATACCT GGTAACGAGG CACGCAGATG
     2761 TTATACCTGT GCGTAGAAGG GGTGACAGTA GAGGATCTCT TTTATCCCCC CGTCCCATTA
     2821 GCTACTTAAA GGGTAGCTCT GGGGGGCCGT TGTTATGTCC GGCAGGACAT GCCGTCGGGT
     2881 TATTCAGAGC GGCTGTTTGT ACGAGGGGTG TTGCAAAAGC AGTAGATTTT ATACCGGTTG
     2941 AGAACTTAGA GACAACGATG AGGTCCCCGG TTTTTACAGA CAACTCATCT ccacctgctg
     3001 ttacactgac ccaccccatc accaaaattg acagggaggt tCTCTACCAG GAATTTGACG
     3061 AAATGGAGGA GTGCTCACAA CACTATCCAT ACGACGTACC AGATTATGCT ggATCCccgg
     3121 cggatgctct agacgatttc gatctggata tgctaccagc cgatgcactt gacgatttcg
     3181 acttagacat gcttccggcc gacgctctag atgacttcga cttagatatg ctcggttccg
     3241 gcgcgaacaa tacaccattt ccgggatatt ttgggggcct tgatttattc gactacgact
     3301 tccttttcgg caacgacttt gctggctcag gtgcgaataa tactcctttt ccaggttatt
     3361 ttggaggttt ggatttattt gattatgact ttttgtttgg caatgacttt gcttaatggc
     3421 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
     3481 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
     3541 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc
     3601 tctaccggca tgccgagcaa atgcctgcaa atcgctcccc atttcgctgC CAAtgagacg
     3661 acggggtcat cacggctcat catgcgccaa acaaatgtgt gcaatacacg ctcggatgac
     3721 tgcatgatga ccgcactgac tggggacagc agatccacct aagcctgtga gagaagcaga
     3781 cacccgacag atcaaggcag ttaactagtg cactgcagta cagcggccgc gattatcaaa
     3841 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3901 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3961 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4021 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc
     4081 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4141 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4201 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4261 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4321 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4381 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4441 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4501 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4561 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4621 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4681 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4741 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4801 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgtcat
     4921 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4981 caaaggatct tc
//