LOCUS       1_pCMV_SS_HA_V5_        7363 bp    DNA        circular     04-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Heegwang Roh
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Jan 18, 2025 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /locus_tag="source:synthetic DNA construct"
                     /label="source:synthetic DNA construct"
                     /ApEinfo_label="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="#00DF87"
                     /ApEinfo_revcolor="#00DF87"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden
     enhancer        10..389
                     /note="human cytomegalovirus immediate early enhancer"
                     /locus_tag="CMV enhancer"
                     /label="CMV enhancer"
                     /ApEinfo_label="CMV enhancer"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        390..593
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /locus_tag="CMV promoter"
                     /label="CMV promoter"
                     /ApEinfo_label="CMV promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        638..656
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     sig_peptide     737..799
                     /product="leader sequence from mouse immunoglobulin kappa
                     light chain"
                     /locus_tag="Ig-kappa leader"
                     /label="Ig-kappa leader"
                     /ApEinfo_label="Ig-kappa leader"
                     /ApEinfo_fwdcolor="#00D95A"
                     /ApEinfo_revcolor="#00D95A"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             800..826
                     /codon_start="1"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /locus_tag="HA"
                     /label="HA"
                     /ApEinfo_label="HA"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             854..895
                     /codon_start="1"
                     /product="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
                     /locus_tag="V5 tag"
                     /label="V5 tag"
                     /ApEinfo_label="V5 tag"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    902..2395
                     /locus_tag="LaccID"
                     /label="LaccID"
                     /ApEinfo_label="LaccID"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2414..3091
                     /locus_tag="Human CD4"
                     /label="Human CD4"
                     /ApEinfo_label="Human CD4"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     polyA_signal    3119..3343
                     /note="bovine growth hormone polyadenylation signal"
                     /locus_tag="bGH poly(A) signal"
                     /label="bGH poly(A) signal"
                     /ApEinfo_label="bGH poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(3475..4063)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     polyA_signal    4392..4439
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /locus_tag="HSV TK poly(A) signal"
                     /label="HSV TK poly(A) signal"
                     /ApEinfo_label="HSV TK poly(A) signal"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(4671..5459)
                     /codon_start="1"
                     /product="aminoglycoside phosphotransferase"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEH
                     QGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /locus_tag="NeoR/KanR"
                     /label="NeoR/KanR"
                     /ApEinfo_label="NeoR/KanR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(5494..5854)
                     /note="SV40 enhancer and early promoter"
                     /locus_tag="SV40 promoter"
                     /label="SV40 promoter"
                     /ApEinfo_label="SV40 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      5508..5646
                     /note="SV40 origin of replication"
                     /locus_tag="SV40 ori"
                     /label="SV40 ori"
                     /ApEinfo_label="SV40 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(5914..6774)
                     /codon_start="1"
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(6775..6879)
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      6906..7361
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /locus_tag="f1 ori"
                     /label="f1 ori"
                     /ApEinfo_label="f1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgact atccatatga tgttccagat tatgctgggg cccagccggc
      841 cagatctggc ggcggcaagc ccatccccaa ccccctgctg ggcctggaca gcaccgctag
      901 cgcgatagga cctgtggcag accttcacat cgccaatgca gacatcagcc cggatgggtt
      961 cactcgttcc gctgtgctag cgggtggaac atttcccggt cccttaataa cgggtaataa
     1021 aggagataat ttccggataa acgtcatcaa ccagcttacg gacacgacta tgttgaggtc
     1081 aaccagtata cattggcacg gattctttca acacgggaca agttgggctg acggccccgc
     1141 tttcgtcact cagtgcccca tcgcaccggg taacagtttt ctttacaact tcactgtccc
     1201 ggatcaagcg ggcacgttct ggtatcatag tcacctaagt acccagtatt gtgacggact
     1261 tagtggtccg tttgtagttt acgaccccaa cgatccccat cgtagcttat acgatgtgga
     1321 tgatgaaagc actgtaatta ctcttgcgga ctggtaccac accccggctc cccagttggc
     1381 cgctgcgccg cccgtcccag atagcaccct aataaacggg aaaggaaggt atgccggagg
     1441 cccaacgtcc ccgttagccg tgataaacgt agagcaggga aaaagatata ggtttcgtct
     1501 tatatctatt tcctgtgatc cgaattttac atttagcatt gatggccata atatgactat
     1561 cattgaagct gatggggtga acacgcaacc actgaccgtg gactctatcc agattttcgc
     1621 tggccaaaga tattccttcg tcctgaatgc gaaccagcct gttggtaact attggataag
     1681 agcgaatcca aatataggaa cgacaggatt cgcaggtggt attaatagtg cgatcctacg
     1741 ttatgtagga gctccagaag cagaccctac aacgacccaa acaacatcca caaatccagt
     1801 gaacgaaaca aacctacacc ccttggagaa ccccggagcc ccggggaatc cgactccggg
     1861 tggtgctgat gtgaacatca accttaacat cggattctcc tcaacgggac aattcaccat
     1921 aaacggtgtt tccttcatac cacccaccgt tcccgtcttg ttgcaaatcc tgtccggggc
     1981 gcgtacggca caagatctgc ttcctagcgg ctctgtttat acattaccgc cgaacaaagt
     2041 catagaaatt agtatgccag gcggcgctag cgcagccggg gggcctcacc cgttccattt
     2101 gcacggccac acttttgatg tggtacgttc agcgggatca tcaacttaca actacgtgaa
     2161 tccagtcaga agagatgtcg tctccattgg aacggcggga gataacgtga caattaggtt
     2221 cactacggac aacccgggtc catggttcct acattgtcat atcgactggc atcttgaggc
     2281 cggtttagcc gtcgtattcg ctgaagacac acctgatatc gcaagcgcta atcccgttcc
     2341 ggctgcgtgg gacgacctat gccctattta tgatgcgtta agccccgacg atcaaccgcg
     2401 gctgcaggtc gacctcgact tccagaaggc ctccagcata gtctataaga aagaggggga
     2461 acaggtggag ttctccttcc cactcgcctt tacagttgaa aagctgacgg gcagtggcga
     2521 gctgtggtgg caggcggaga gggcttcctc ctccaagtct tggatcacct ttgacctgaa
     2581 gaacaaggaa gtgtctgtaa aacgggttac ccaggaccct aagctccaga tgggcaagaa
     2641 gctcccgctc cacctcaccc tgccccaggc cttgcctcag tatgctggct ctggaaacct
     2701 caccctggcc cttgaagcga aaacaggaaa gttgcatcag gaagtgaacc tggtggtgat
     2761 gagagccact cagctccaga aaaatttgac ctgtgaggtg tggggaccca cctcccctaa
     2821 gctgatgctg agcttgaaac tggagaacaa ggaggcaaag gtctcgaagc gggagaaggc
     2881 ggtgtgggtg ctgaaccctg aggcggggat gtggcagtgt ctgctgagtg actcgggaca
     2941 ggtcctgctg gaatccaaca tcaaggttct gcccacatgg tccaccccgg tgcagccaat
     3001 ggccctgatt gtgctggggg gcgtcgccgg cctcctgctt ttcattgggc taggcatctt
     3061 cttctgtgtc aggtgccggc accgaaggcg ctaggcggcc gctcgagatc agcctcgact
     3121 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3181 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3241 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3301 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     3361 accagtggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3421 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3481 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3541 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3601 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3661 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3721 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3781 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3841 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3901 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3961 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4021 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4081 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4201 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
     4261 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
     4321 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     4381 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
     4441 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
     4501 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     4561 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     4621 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     4681 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     4741 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     4801 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     4861 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     4921 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     4981 tgctcttgat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     5041 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     5101 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     5161 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     5221 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc
     5281 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     5341 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     5401 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     5461 cgaaacgatc ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa
     5521 gcctcctcac tacttctgga atagctcaga ggccgaggag gcggcctcgg cctctgcata
     5581 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     5641 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     5701 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     5761 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     5821 acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca ataaatcaat
     5881 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5941 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6001 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6061 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6121 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6181 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6241 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6301 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6361 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6421 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6481 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6541 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6601 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6661 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6721 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6781 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6841 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6901 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6961 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7021 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7081 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     7141 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     7201 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     7261 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     7321 atttaacgcg aattttaaca aaatattaac gcttacaatt tac 
//
