LOCUS       4_Aga2p_TEVcs_La        7687 bp    DNA        circular     04-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Heegwang Roh
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Jan 18, 2025 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          join(1..3944,5439..7687)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /locus_tag="source:synthetic DNA construct"
                     /label="source:synthetic DNA construct"
                     /ApEinfo_label="source:synthetic DNA construct"
                     /ApEinfo_fwdcolor="#00DF87"
                     /ApEinfo_revcolor="#00DF87"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden
     misc_feature    69..572
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
                     /locus_tag="CEN/ARS"
                     /label="CEN/ARS"
                     /ApEinfo_label="CEN/ARS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        609..713
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label="AmpR promoter"
                     /ApEinfo_label="AmpR promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             714..1574
                     /codon_start="1"
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      1745..2333
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /locus_tag="ori"
                     /label="ori"
                     /ApEinfo_label="ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    2621..2642
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /locus_tag="CAP binding site"
                     /label="CAP binding site"
                     /ApEinfo_label="CAP binding site"
                     /ApEinfo_fwdcolor="#00D95A"
                     /ApEinfo_revcolor="#00D95A"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        2657..2687
                     /note="promoter for the E. coli lac operon"
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    2695..2711
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /locus_tag="lac operator"
                     /label="lac operator"
                     /ApEinfo_label="lac operator"
                     /ApEinfo_fwdcolor="#008065"
                     /ApEinfo_revcolor="#008065"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2719..2735
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 rev"
                     /label="M13 rev"
                     /ApEinfo_label="M13 rev"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        2756..2774
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        3008..3448
                     /gene="S. cerevisiae GAL1"
                     /note="inducible promoter, regulated by Gal4"
                     /locus_tag="GAL1 promoter"
                     /label="GAL1 promoter"
                     /ApEinfo_label="GAL1 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    3008..3125
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
                     /locus_tag="UAS"
                     /label="UAS"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#13AAFE"
                     /ApEinfo_revcolor="#13AAFE"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3495..3755
                     /locus_tag="Aga2p"
                     /label="Aga2p"
                     /ApEinfo_label="Aga2p"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3774..3785
                     /codon_start="1"
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /locus_tag="Factor Xa site"
                     /label="Factor Xa site"
                     /ApEinfo_label="Factor Xa site"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3786..3812
                     /codon_start="1"
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
                     /locus_tag="HA"
                     /label="HA"
                     /ApEinfo_label="HA"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3813..3854
                     /codon_start="1"
                     /product="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
                     /locus_tag="V5 tag"
                     /label="V5 tag"
                     /ApEinfo_label="V5 tag"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3855..3875
                     /codon_start="1"
                     /product="tobacco etch virus (TEV) protease recognition
                     and cleavage site"
                     /translation="ENLYFQS"
                     /locus_tag="TEV site"
                     /label="TEV site"
                     /ApEinfo_label="TEV site"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3888..3932
                     /locus_tag="(Gly4Ser)3"
                     /label="(Gly4Ser)3"
                     /ApEinfo_label="(Gly4Ser)3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             5445..5474
                     /codon_start="1"
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
                     /locus_tag="Myc"
                     /label="Myc"
                     /ApEinfo_label="Myc"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(5808..5826)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /locus_tag="T7 promoter"
                     /label="T7 promoter"
                     /ApEinfo_label="T7 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(5833..5849)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /locus_tag="M13 fwd"
                     /label="M13 fwd"
                     /ApEinfo_label="M13 fwd"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      5994..6449
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /locus_tag="f1 ori"
                     /label="f1 ori"
                     /ApEinfo_label="f1 ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(6546..7220)
                     /codon_start="1"
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required
                     for tryptophan biosynthesis"
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK"
                     /locus_tag="TRP1"
                     /label="TRP1"
                     /ApEinfo_label="TRP1"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(7221..7501)
                     /gene="S. cerevisiae TRP1"
                     /locus_tag="TRP1 promoter"
                     /label="TRP1 promoter"
                     /ApEinfo_label="TRP1 promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3945..5438
                     /locus_tag="LaccID"
                     /label="LaccID"
                     /ApEinfo_label="LaccID"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
      241 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
      301 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
      361 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
      421 aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta
      481 ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
      541 tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta
     2761 accctcacta aagggaacaa aagctggtac caattccttg aattttcaaa aattcttact
     2821 ttttttttgg atggacgcaa agaagtttaa taatcatatt acatggcatt accaccatat
     2881 acatatccat atctaatctt acttatatgt tgtggaaatg taaagagccc cattatctta
     2941 gcctaaaaaa accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat
     3001 tgaagtacgg attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc
     3061 gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg
     3121 ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc
     3181 agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata
     3241 atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg
     3301 atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac
     3361 attttcggtt tgtattactt cttattcaaa tgtaataaaa gatcaacaaa aaattgttaa
     3421 tatacctcta tactttaacg tcaaggagaa aaaaccccgg atcgaattcc ctacttcata
     3481 cattttcaat taagatgcag ttacttcgct gtttttcaat attttctgtt attgcttcag
     3541 ttttagcaca ggaactgaca actatatgcg agcaaatccc ctcaccaact ttagaatcga
     3601 cgccgtactc tttgtcaacg actactattt tggccaacgg gaaggcaatg caaggagttt
     3661 ttgaatatta caaatcagta acgtttgtca gtaattgcgg ttctcacccc tcaacaacta
     3721 gcaaaggcag ccccataaac acacagtatg tttttaagga caatagctcg acgattgaag
     3781 gtagataccc atacgacgtt ccagactacg ctggcaagcc catccccaac cccctgctgg
     3841 gcctggacag caccgagaac ttgtactttc aatctctgca ggctagtggt ggaggaggct
     3901 ctggtggagg cggtagcgga ggcggagggt cggctagcca tatggcgata ggacctgtgg
     3961 cagaccttca catcgCcaat gcagacatca gcccggatgg gttcactcgt tccgctgtgc
     4021 tagcgggtgg aacatttccc ggtcccttaa taacgggtaa taaaggagat aatttccGga
     4081 taaacgtcat caaccagctt acggacacga ctatgttgag gtcaaccagt atacattggc
     4141 acggattctt tcaacacggg acaaGttggg ctgacggccc cgctttcgtc actcagtgcc
     4201 ccatcgcacc gggtaacagt tttctttaca acttcactgt cccggatcaa gcgggcacgt
     4261 tctggtatca tagtcaccta agtacccagt attgtgacgg acttAgtggt ccgtttgtaG
     4321 tttacgaccc caacgatccc catcgtagct tatacgatgt ggatgatgaa agcactgtaa
     4381 ttactcttgc ggactggtac cacaccccgg ctccccagtt ggccgctgcg ccgcccgtcc
     4441 cagatagcac cctaataaac gggaaaggaa ggtatgccgg aggcccaacg tccccgttag
     4501 ccgtgataaa cgtagagcag ggaaaaagat ataggtttcg tcttatatct atttcctgtg
     4561 atccgaattt tacatttagc attgatggcc ataatatgac tatcattgaa gctgatgggg
     4621 tgaacacgca accactgacc gtggactcta tccagatttt cgctggccaa agatattcct
     4681 tcgtcctgaa tgcgaaccag cctgttgGta actattggat aagagcgaat ccaaatatag
     4741 gaacgacagg attcgcaggt ggtattaata gtgcgatcct acgttatgta ggagctccag
     4801 aagcagaccc tacaacgacc caaacaacat ccacaaatcc aGtgaacgaa acaaacctac
     4861 accccttgga gaaccccgga gccccgggga atccgactcc gggtggtgct gatgtgaaca
     4921 tcaaccttaa catcggattc tcctcaacgg gacaattcac cataaacggt gtttccttca
     4981 taccacccac cgttcccgtc ttgttgcaaa tcctgtccgg ggcgcgtacg gcacaagatc
     5041 tgcttcctag cggctctgtt tatacattac cgccgaacaa agtcatagaa attagtatgc
     5101 caggcggcgc tagcgcagcc ggggggcctc acccgttcca tttgcacggc cacacttttg
     5161 atgtggtacg ttcagcggga tcatcaactt acaactacgt gaatccagtc agaagagatg
     5221 tcgtctccat tggaacggcg ggagataacg tgacaattag gttcactacg gacaacccgg
     5281 gtccatggtt cctacattgt catatcgact ggcatcttga ggccggttta gccgtcgtat
     5341 tcgctgaaga cacacctgat atcgcaagcg ctaatcccgt tccggctgcg tgggacgacc
     5401 tatgccctat ttatgatgcg ttaagccccg acgatcaagg atccgaacaa aagcttattt
     5461 ctgaagagga cttgtaatag ctcgagatct gataacaaca gtgtagatgt aacaaaatcg
     5521 actttgttcc cactgtactt ttagctcgta caaaatacaa tatacttttc atttctccgt
     5581 aaacaacatg ttttcccatg taatatcctt ttctattttt cgttccgtta ccaactttac
     5641 acatacttta tatagctatt cacttctata cactaaaaaa ctaagacaat tttaattttg
     5701 ctgcctgcca tatttcaatt tgttataaat tcctataatt tatcctatta gtagctaaaa
     5761 aaagatgaat gtgaatcgaa tcctaagaga attgagctcc aattcgccct atagtgagtc
     5821 gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     5881 ccaacttaat cgccttgcag cacatccccc cttcgccagc tggcgtaata gcgaagaggc
     5941 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc
     6001 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6061 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6121 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6181 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6241 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6301 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6361 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6421 ttttaacaaa atattaacgt ttacaatttc ctgatgcggt attttctcct tacgcatctg
     6481 tgcggtattt cacaccgcag gcaagtgcac aaacaatact taaataaata ctactcagta
     6541 ataacctatt tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca
     6601 tttgtctcca cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca
     6661 ccaacatttt ctggcgtcag tccaccagct aacataaaat gtaagctttc ggggctctct
     6721 tgccttccaa cccagtcaga aatcgagttc caatccaaaa gttcacctgt cccacctgct
     6781 tctgaatcaa acaagggaat aaacgaatga ggtttctgtg aagctgcact gagtagtatg
     6841 ttgcagtctt ttggaaatac gagtctttta ataactggca aaccgaggaa ctcttggtat
     6901 tcttgccacg actcatctcc atgcagttgg acgatatcaa tgccgtaatc attgaccaga
     6961 gccaaaacat cctccttagg ttgattacga aacacgccaa ccaagtattt cggagtgcct
     7021 gaactatttt tatatgcttt tacaagactt gaaattttcc ttgcaataac cgggtcaatt
     7081 gttctctttc tattgggcac acatataata cccagcaagt cagcatcgga atctagagca
     7141 cattctgcgg cctctgtgct ctgcaagccg caaactttca ccaatggacc agaactacct
     7201 gtgaaattaa taacagacat actccaagct gcctttgtgt gcttaatcac gtatactcac
     7261 gtgctcaata gtcaccaatg ccctccctct tggccctctc cttttctttt ttcgaccgaa
     7321 ttaattctta atcggcaaaa aaagaaaagc tccggatcaa gattgtacgt aaggtgacaa
     7381 gctatttttc aataaagaat atcttccact actgccatct ggcgtcataa ctgcaaagta
     7441 cacatatatt acgatgctgt ctattaaatg cttcctatat tatatatata gtaatgtcgt
     7501 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     7561 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7621 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7681 cgcgcga     
//
