LOCUS       pRWC014_C10        4343 bp DNA     circular SYN 26-AUG-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      216..302
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      394..421
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        440..531
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             532..1392
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  532 ..  600 / #ccffcc / signal sequence
                        2:  601 .. 1392 / #ccffcc
                     Cleavage site after base 600"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1563..2151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    2389..2529
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     CDS             complement(2793..3671)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /note="color: #993366"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3698..3982
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4014..4343
                     /label=C10
                     /note="color: #993366; direction: RIGHT"
     CDS             4323..4340
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
ORIGIN
        1 gaattcgaag cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa
       61 atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt
      121 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
      181 gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
      241 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      301 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
      361 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
      421 ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc
      481 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      541 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      601 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      661 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      721 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
      781 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      841 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      901 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      961 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1021 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1081 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1141 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1201 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1261 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1321 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1381 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1441 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1501 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1561 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1621 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1681 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1741 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1801 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1861 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1921 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1981 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2041 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2101 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2161 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2221 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2281 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     2341 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgagcgcc tgatgcggta
     2401 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2461 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2521 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2581 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2641 tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa ggcgaagcgg
     2701 catgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc
     2761 gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc
     2821 actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat
     2881 acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
     2941 atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt
     3001 aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa
     3061 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
     3121 tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc
     3181 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct
     3241 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
     3301 tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc
     3361 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga
     3421 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca
     3481 aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa
     3541 cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
     3601 gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc
     3661 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
     3721 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc
     3781 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
     3841 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
     3901 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
     3961 caactctcta ctgtttctcc atacccgttt tttgggctaa caggaggaat taaatgaatg
     4021 aaaaaaaaga aaaagcacgt aaagaaatga aagagattgc cgagaaggca atcaaagaaa
     4081 ttaaaaaaga tccggaaaaa gctctggaaa tcgcagtgaa ggcggttgaa gaaatcggtg
     4141 agattgcgaa cgaaagcggc gattataaga gtggtgagga gaatgcttgg aaggtcgctg
     4201 aagaatacgc gaaagtaaca ggcgacgatg acaccgccta tgatcttatg ttggccgggg
     4261 atatgattat agacggaaag gatgcagagg aaggatatga gatattaaaa gaactgggtt
     4321 cacatcacca tcatcatcac taa
//
