LOCUS       PHR_CMV_Melt40-m       11433 bp DNA     circular SYN 07-APR-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Apr 8, 2025 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    231..348
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     enhancer        438..741
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        742..945
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      983..988
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             989..2491
                     /codon_start=1
                     /label=BcLOV4
                     /note="color: #00ffff"
                     /translation="MAGSNGNGLGLSSAGNFADFFSSEVFYIVLHNPTTAHRFLKFCQS
                     RACSENLEFLQKIDEYNRLIDEATRLLSTIYSTYVSSEAPKQINISNSHARRLSHDIKG
                     ATHNVLPGLEDIFNGSQEQIEKLLASDLYPRFVKHQVTASATMALANDKERYPGLGDCF
                     CLTDPRRADNPIVFASDGFVSVTGYSRSDIIPRNARFLQGSFTDRQATKRLRTSIENRE
                     ETVELLLNYRKNGDPFWNLLYVSPLLDGNGDVRFFLGGNINCSTTIHSRTDVLRILSLN
                     DEDTGSFIEGSNKTPSVRSKESNNASNYGKTSFFKSFKKYKANTVEIRDEAGMEGELID
                     RIGKLQFKTQVEEFYTAYSKYLVLSYQPQSQNLIVKYFSPGIVDMLNLNMPNGLVAPIV
                     DKDIFKVLTEHSPSSVPRTFKNVVREGIKAGRAVSVETGLLTGIEEIKKSQNIFSGTTA
                     NRERGWKKAEEKYVCHFTPCKDEMGRVGWVVLTVAPKLERFEF"
     primer_bind     complement(2457..2491)
                     /label=BcLOV4 HF R
                     /note="color: black; sequence: 
                     GAATTCAAATCTTTCCAGTTTAGGAGCGACAGTCA; added: 2021-09-22"
     primer_bind     complement(2492..2512)
                     /label=FlexLinker-xhoI R
                     /note="color: black; sequence: 
                     agtcggCTCGAGTCCTCCACCTGATCCACCACC; added: 2021-09-25"
     CDS             2516..2575
                     /codon_start=1
                     /label=stim
                     /note="color: #ff9900"
                     /translation="DSSPGRKKFPLKIFKKPLKK"
     primer_bind     2597..2618
                     /label=mCh-xhoI F
                     /note="color: black; sequence: 
                     aatccgCTCGAGTCTATGGTGAGCAAGGGCGAGG; added: 2021-09-25"
     CDS             2600..3307
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             3317..3337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             3362..3406
                     /codon_start=1
                     /label=NES strada
                     /note="color: #993366"
                     /translation="GIFGLVTNLEELEVD"
     primer_bind     complement(3439..3463)
                     /label=ZDH #117 pHR NotI HF R
                     /note="color: black; sequence: cacgcatgttgcaggtgggagttgc; 
                     added: 2023-02-07"
     misc_feature    3530..4118
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4001..4012)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4000"
                     /translation="IEGR"
     LTR             4181..4414
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        complement(4439..4457)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     promoter        5246..5350
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(5351..5419,5420..6211)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5351 .. 5419 / #ccffcc / signal sequence
                        2: 5420 .. 6211 / #ccffcc
                     Cleavage site after base 5419"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6382..6970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        7216..7545
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      7396..7531
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     intron          8679..8744
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /note="color: #------"
     CDS             8874..8894
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    9319..9453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     LTR             9622..10255
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    10302..10427
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    10919..11152
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
ORIGIN
        1 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
       61 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      121 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      181 gattagtgaa cggatctcga cggtcgccaa atggcagtat tcatccacaa ttttaaaaga
      241 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      301 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      361 agggacagca gagatccagt ttggatcgat aagcttgata tcgaattctt agttcatagc
      421 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      481 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      541 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      601 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      661 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      721 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      781 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      841 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      901 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      961 cagatccgct agcgctacgc gtgccaccat ggccggctct aacggaaatg gcctgggcct
     1021 gtcctctgcc ggcaacttcg ccgatttctt tagctccgag gtgttttata tcgtgctgca
     1081 caatcccacc acagcccaca gattcctgaa gttttgccag agcagggcct gttccgagaa
     1141 cctggagttt ctgcagaaga tcgacgagta caataggctg atcgatgagg ccacacgcct
     1201 gctgtctacc atctacagca catacgtgag cagcgaggcc cctaagcaga tcaacatctc
     1261 taatagccac gccaggcgcc tgagccacga catcaaggga gcaacccaca acgtgctgcc
     1321 cggcctggag gatatcttca atggctccca ggagcagatc gagaagctgc tggcctctga
     1381 cctgtatcct agatttgtga agcaccaggt gaccgcatcc gccacaatgg ccctggcaaa
     1441 cgacaaggag aggtacccag gcctgggcga ttgcttctgt ctgacagacc cacggagagc
     1501 cgataatccc atcgtgttcg caagcgacgg attcgtgagc gtgaccggct attcccgctc
     1561 tgatatcatc ccaaggaacg cccgcttcct gcagggcagc tttaccgacc ggcaggccac
     1621 aaagaggctg cgcacctcca tcgagaatcg cgaggagaca gtggagctgc tgctgaacta
     1681 tcggaagaat ggcgatccct tttggaacct gctgtacgtg tcccctctgc tggacggcaa
     1741 tggcgatgtg agattctttc tgggcggcaa tatcaactgt tctaccacaa tccacagccg
     1801 gaccgacgtg ctgagaatcc tgagcctgaa cgacgaggat acaggctcct tcatcgaggg
     1861 ctctaataag acccccagcg tgcggtccaa ggagtctaac aatgcctcca actatggcaa
     1921 gacatccttc tttaagtctt ttaagaagta caaggccaat accgtggaga tcagagacga
     1981 ggccggcatg gagggcgagc tgatcgatag gatcggcaag ctgcagttca agacacaggt
     2041 ggaggagttt tacaccgcct attctaagta cctggtgctg agctatcagc cacagtccca
     2101 gaacctgatc gtgaagtact tcagccccgg catcgtggat atgctgaacc tgaatatgcc
     2161 taatggcctg gtggccccaa tcgtggacaa ggatatcttc aaggtgctga cagagcactc
     2221 cccttcctct gtgccacgga cctttaagaa cgtggtgagg gaaggcatca aagcaggcag
     2281 ggccgtgtct gtggagacag gcctgctgac aggcatcgag gagatcaaga agtctcagaa
     2341 catcttcagc ggcaccacag ccaatcggga gagaggctgg aagaaggccg aggagaagta
     2401 cgtgtgccac tttaccccct gtaaggatga gatgggcagg gtcggctggg tggtgctgac
     2461 tgtcgctcct aaactggaaa gatttgaatt cggtggtgga tcaggtggag gatctgattc
     2521 ttcccctgga cgaaagaaat ttccgttgaa gatctttaag aaaccactga agaagggtgg
     2581 aggaagcggc ggcggttcta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     2641 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     2701 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     2761 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     2821 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     2881 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     2941 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     3001 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     3061 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     3121 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     3181 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     3241 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     3301 gtacaagggc tcttccccga aaaagaaacg caaagttggc ggtggatctg gtggcggttc
     3361 tggcattttt ggcctggtga ccaacctgga agaactggaa gtggattaat ctagagggcc
     3421 cgtttaaacc cggcggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga
     3481 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
     3541 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3601 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3661 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3721 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3781 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3841 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3901 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     3961 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4021 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4081 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gaccaaggca
     4141 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4201 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     4261 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4321 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4381 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     4441 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     4501 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     4561 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     4621 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     4681 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     4741 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     4801 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     4861 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     4921 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     4981 acaatctagc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5041 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5101 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     5161 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5221 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5281 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5341 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5401 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5461 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5521 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5581 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5641 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5701 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5761 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5821 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5881 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5941 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6001 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6061 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6121 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6181 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6241 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6301 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6361 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6421 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6481 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6541 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6601 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6661 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6721 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6781 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6841 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6901 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6961 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7021 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7081 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7141 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7201 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7261 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7321 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7381 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7441 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7501 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
     7561 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     7621 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     7681 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     7741 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     7801 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     7861 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     7921 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
     7981 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
     8041 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     8101 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
     8161 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     8221 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     8281 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     8341 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     8401 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     8461 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
     8521 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
     8581 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     8641 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     8701 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     8761 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     8821 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     8881 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     8941 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     9001 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     9061 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     9121 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     9181 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     9241 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     9301 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9361 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9421 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     9481 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
     9541 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     9601 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     9661 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     9721 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     9781 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     9841 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     9901 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     9961 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
    10021 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
    10081 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10141 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10201 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
    10261 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
    10321 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
    10381 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
    10441 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
    10501 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
    10561 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    10621 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    10681 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    10741 aaagtaagac caccgcacag caagcggccg gtgatcttca gacctggacg atatatatga
    10801 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    10861 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
    10921 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    10981 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    11041 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    11101 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    11161 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    11221 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    11281 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    11341 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    11401 caaattggct gtggtatata aaattattca taa
//
