LOCUS       Exported                4739 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC033867*
               Fragment ends: L1fake and L2fake
               Fragment size: 1554
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..2221
                     /codon_start=1
                     /label=tev-Hs.ACVR1
                     /note="ACVR1 protein"
                     /translation="ENLYFQGTMVDGVMILPVLIMIALPSPSMEDEKPKVNPKLYMCVC
                     EGLSCGNEDHCEGQQCFSSLSINDGFHVYQKGCFQVYEQGKMTCKTPPSPGQAVECCQG
                     DWCNRNITAQLPTKGKSFPGTQNFHLEVGLIILSVVFAVCLLACLLGVALRKFKRRNQE
                     RLNPRDVEYGTIEGLITTNVGDSTLADLLDHSCTSGSGSGLPFLVQRTVARQITLLECV
                     GKGRYGEVWRGSWQGENVAVKIFSSRDEKSWFRETELYNTVMLRHENILGFIASDMTSR
                     HSSTQLWLITHYHEMGSLYDYLQLTTLDTVSCLRIVLSIASGLAHLHIEIFGTQGKPAI
                     AHRDLKSKNILVKKNGQCCIADLGLAVMHSQSTNQLDVGNNPRVGTKRYMAPEVLDETI
                     QVDCFDSYKRVDIWAFGLVLWEVARRMVSNGIVEDYKPPFYDVVPNDPSFEDMRKVVCV
                     DQQRPNIPNRWFSDPTLTSLAKLMKECWYQNPSARLTALRIKKTLTKIDNSLDKLKTDC
                     "
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2220..2319)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2337..2355)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2360..2376)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2807..3598
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3720..3829
                     /note="incP origin of transfer"
     rep_origin      4089..4677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catggtagat ggagtgatga ttcttcctgt
      721 gcttatcatg attgctctcc cctcccctag tatggaagat gagaagccca aggtcaaccc
      781 caaactctac atgtgtgtgt gtgaaggtct ctcctgcggt aatgaggacc actgtgaagg
      841 ccagcagtgc ttttcctcac tgagcatcaa cgatggcttc cacgtctacc agaaaggctg
      901 cttccaggtt tatgagcagg gaaagatgac ctgtaagacc ccgccgtccc ctggccaagc
      961 tgtggagtgc tgccaagggg actggtgtaa caggaacatc acggcccagc tgcccactaa
     1021 aggaaaatcc ttccctggaa cacagaattt ccacttggag gttggcctca ttattctctc
     1081 tgtagtgttc gcagtatgtc ttttagcctg cctgctggga gttgctctcc gaaaatttaa
     1141 aaggcgcaac caagaacgcc tcaatccccg agacgtggag tatggcacta tcgaagggct
     1201 catcaccacc aatgttggag acagcacttt agcagattta ttggatcatt cgtgtacatc
     1261 aggaagtggc tctggtcttc cttttctggt acaaagaaca gtggctcgcc agattacact
     1321 gttggagtgt gtcgggaaag gcaggtatgg tgaggtgtgg aggggcagct ggcaagggga
     1381 aaatgttgcc gtgaagatct tctcctcccg tgatgagaag tcatggttca gggaaacgga
     1441 attgtacaac actgtgatgc tgaggcatga aaatatctta ggtttcattg cttcagacat
     1501 gacatcaaga cactccagta cccagctgtg gttaattaca cattatcatg aaatgggatc
     1561 gttgtacgac tatcttcagc ttactactct ggatacagtt agctgccttc gaatagtgct
     1621 gtccatagct agtggtcttg cacatttgca catagagata tttgggaccc aagggaaacc
     1681 agccattgcc catcgagatt taaagagcaa aaatattctg gttaagaaga atggacagtg
     1741 ttgcatagca gatttgggcc tggcagtcat gcattcccag agcaccaatc agcttgatgt
     1801 ggggaacaat ccccgtgtgg gcaccaagcg ctacatggcc cccgaagttc tagatgaaac
     1861 catccaggtg gattgtttcg attcttataa aagggtcgat atttgggcct ttggacttgt
     1921 tttgtgggaa gtggccaggc ggatggtgag caatggtata gtggaggatt acaagccacc
     1981 gttctacgat gtggttccca atgacccaag ttttgaagat atgaggaagg tagtctgtgt
     2041 ggatcaacaa aggccaaaca tacccaacag atggttctca gacccgacat taacctctct
     2101 ggccaagcta atgaaagaat gctggtatca aaatccatcc gcaagactca cagcactgcg
     2161 tatcaaaaag actttgacca aaattgataa ttccctcgac aaattgaaaa ctgactgtta
     2221 atcaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2281 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     2341 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     2401 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     2461 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     2521 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     2581 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     2641 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     2701 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     2761 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     2821 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     2881 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     2941 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     3001 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     3061 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     3121 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     3181 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     3241 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     3301 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     3361 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     3421 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     3481 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     3541 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     3601 ctcgactcta gaggatcccc gggtaccgag ctcgaattcc gtgataggtg ggctgccctt
     3661 cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc
     3721 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
     3781 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
     3841 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa
     3901 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga
     3961 aaacggaatt cgagctcggt acccggggat cctctagagt cgagccaccc atgaccaaaa
     4021 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4081 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4141 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4201 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4261 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4321 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4381 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4441 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     4501 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4561 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4621 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4681 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
