LOCUS       Exported                4808 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC028383*
               Fragment ends: L1fake and L2fake
               Fragment size: 1623
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..2290
                     /codon_start=1
                     /label=tev-Hs.BMPR1A
                     /note="bone morphogenetic protein receptor, type IA"
                     /translation="ENLYFQGTMPQLYIYIRLLGAYLFIISRVQGQNLDSMLHGTGMKS
                     DSDQKKSENGVTLAPEDTLPFLKCYCSGHCPDDAINNTCITNGHCFAIIEEDDQGETTL
                     ASGCMKYEGSDFQCKDSPKAQLRRTIECCRTNLCNQYLQPTLPPVVIGPFFDGSIRWLV
                     LLISMAVCIIAMIIFSSCFCYKHYCKSISSRRRYNRDLEQDEAFIPVGESLKDLIDQSQ
                     SSGSGSGLPLLVQRTIAKQIQMVRQVGKGRYGEVWMGKWRGEKVAVKVFFTTEEASWFR
                     ETEIYQTVLMRHENILGFIAADIKGTGSWTQLYLITDYHENGSLYDFLKCATLDTRALL
                     KLAYSAACGLCHLHTEIYGTQGKPAIAHRDLKSKNILIKKNGSCCIADLGLAVKFNSDT
                     NEVDVPLNTRVGTKRYMAPEVLDESLNKNHFQPYIMADIYSFGLIIWEMARRCITGGIV
                     EEYQLPYYNMVPSDPSYEDMREVVCVKRLRPIVSNRWNSDECLRAVLKLMSECWAHNPA
                     SRLTALRIKKTLAKMVESQDVKI"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2289..2388)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2406..2424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2429..2445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2876..3667
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3789..3898
                     /note="incP origin of transfer"
     rep_origin      4158..4746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgcctcag ctatacattt acatcagatt
      721 attgggagcc tatttgttca tcatttctcg tgttcaagga cagaatctgg atagtatgct
      781 tcatggcact gggatgaaat cagactccga ccagaaaaag tcagaaaatg gagtaacctt
      841 agcaccagag gataccttgc cttttttaaa gtgctattgc tcagggcact gtccagatga
      901 tgctattaat aacacatgca taactaatgg acattgcttt gccatcatag aagaagatga
      961 ccagggagaa accacattag cttcagggtg tatgaaatat gaaggatctg attttcagtg
     1021 caaagattct ccaaaagccc agctacgccg gacaatagaa tgttgtcgga ccaatttatg
     1081 taaccagtat ttgcaaccca cactgccccc tgttgtcata ggtccgtttt ttgatggcag
     1141 cattcgatgg ctggttttgc tcatttctat ggctgtctgc ataattgcta tgatcatctt
     1201 ctccagctgc ttttgttaca aacattattg caagagcatc tcaagcagac gtcgttacaa
     1261 tcgtgatttg gaacaggatg aagcatttat tccagttgga gaatcactaa aagaccttat
     1321 tgaccagtca caaagttctg gtagtgggtc tggactacct ttattggttc agcgaactat
     1381 tgccaaacag attcagatgg tccggcaagt tggtaaaggc cgatatggag aagtatggat
     1441 gggcaaatgg cgtggcgaaa aagtggcggt gaaagtattc tttaccactg aagaagccag
     1501 ctggtttcga gaaacagaaa tctaccaaac tgtgctaatg cgccatgaaa acatacttgg
     1561 tttcatagcg gcagacatta aaggtacagg ttcctggact cagctctatt tgattactga
     1621 ttaccatgaa aatggatctc tctatgactt cctgaaatgt gctacactgg acaccagagc
     1681 cctgcttaaa ttggcttatt cagctgcctg tggtctgtgc cacctgcaca cagaaattta
     1741 tggcacccaa ggaaagcccg caattgctca tcgagaccta aagagcaaaa acatcctcat
     1801 caagaaaaat gggagttgct gcattgctga cctgggcctt gctgttaaat tcaacagtga
     1861 cacaaatgaa gttgatgtgc ccttgaatac cagggtgggc accaaacgct acatggctcc
     1921 cgaagtgctg gacgaaagcc tgaacaaaaa ccacttccag ccctacatca tggctgacat
     1981 ctacagcttc ggcctaatca tttgggagat ggctcgtcgt tgtatcacag gagggatcgt
     2041 ggaagaatac caattgccat attacaacat ggtaccgagt gatccgtcat acgaagatat
     2101 gcgtgaggtt gtgtgtgtca aacgtttgcg gccaattgtg tctaatcggt ggaacagtga
     2161 tgaatgtcta cgagcagttt tgaagctaat gtcagaatgc tgggcccaca atccagcctc
     2221 cagactcaca gcattgagaa ttaagaagac gcttgccaag atggttgaat cccaagatgt
     2281 aaaaatctaa tcaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2341 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2401 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2461 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     2521 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     2581 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     2641 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2701 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     2761 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     2821 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     2881 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     2941 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     3001 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     3061 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     3121 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3181 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3241 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3301 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3361 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3421 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3481 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3541 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3601 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     3661 caaataaccc tcgactctag aggatccccg ggtaccgagc tcgaattccg tgataggtgg
     3721 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
     3781 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
     3841 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
     3901 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
     3961 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
     4021 tgcagcggaa aacggaattc gagctcggta cccggggatc ctctagagtc gagccaccca
     4081 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     4141 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4201 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4261 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4321 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4381 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4441 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4501 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     4561 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4621 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4681 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4741 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4801 cacatgtt
//
