LOCUS       Exported                4727 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC000254*
               Fragment ends: L1fake and L2fake
               Fragment size: 1542
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..2209
                     /codon_start=1
                     /label=tev-Hs.ACVR1B
                     /note="activin A receptor, type IB"
                     /translation="ENLYFQGTMAESAGASSFFPLVVLLLAGSGGSGPRGVQALLCACT
                     SCLQANYTCETDGACMVSIFNLDGMEHHVRTCIPKVELVPAGKPFYCLSSEDLRNTHCC
                     YTDYCNRIDLRVPSGHLKEPEHPSMWGPVELVGIIAGPVFLLFLIIIIVFLVINYHQRV
                     YHNRQRLDMEDPSCEMCLSKDKTLQDLVYDLSTSGSGSGLPLFVQRTVARTIVLQEIIG
                     KGRFGEVWRGRWRGGDVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNG
                     TWTQLWLVSDYHEHGSLFDYLNRYTVTIEGMIKLALSAASGLAHLHMEIVGTQGKPGIA
                     HRDLKSKNILVKKNGMCAIADLGLAVRHDAVTDTIDIAPNQRVGTKRYMAPEVLDETIN
                     MKHFDSFKCADIYALGLVYWEIARRCNSGGVHEEYQLPYYDLVPSDPSIEEMRKVVCDQ
                     KLRPNIPNWWQSYEALRVMGKMMRECWYANGAARLTALRIKKTLSQLSVQEDVKI"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2208..2307)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2325..2343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2348..2364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2795..3586
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3708..3817
                     /note="incP origin of transfer"
     rep_origin      4077..4665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catggcggag tcggccggag cctcctcctt
      721 cttccccctt gttgtcctcc tgctcgccgg cagcggcggg tccgggcccc ggggggtcca
      781 ggctctgctg tgtgcgtgca ccagctgcct ccaggccaac tacacgtgtg agacagatgg
      841 ggcctgcatg gtttccattt tcaatctgga tgggatggag caccatgtgc gcacctgcat
      901 ccccaaagtg gagctggtcc ctgccgggaa gcccttctac tgcctgagct cggaggacct
      961 gcgcaacacc cactgctgct acactgacta ctgcaacagg atcgacttga gggtgcccag
     1021 tggtcacctc aaggagcctg agcacccgtc catgtggggc ccggtggagc tggtaggcat
     1081 catcgccggc ccggtgttcc tcctgttcct catcatcatc attgttttcc ttgtcattaa
     1141 ctatcatcag cgtgtctatc acaaccgcca gagactggac atggaagatc cctcatgtga
     1201 gatgtgtctc tccaaagaca agacgctcca ggatcttgtc tacgatctct ccacctcagg
     1261 gtctggctca gggttacccc tctttgtcca gcgcacagtg gcccgaacca tcgttttaca
     1321 agagattatt ggcaagggtc ggtttgggga agtatggcgg ggccgctgga ggggtggtga
     1381 tgtggctgtg aaaatattct cttctcgtga agaacggtct tggttcaggg aagcagagat
     1441 ataccagacg gtcatgctgc gccatgaaaa catccttgga tttattgctg ctgacaataa
     1501 agataatggc acctggacac agctgtggct tgtttctgac tatcatgagc acgggtccct
     1561 gtttgattat ctgaaccggt acacagtgac aattgagggg atgattaagc tggccttgtc
     1621 tgctgctagt gggctggcac acctgcacat ggagatcgtg ggcacccaag ggaagcctgg
     1681 aattgctcat cgagacttaa agtcaaagaa cattctggtg aagaaaaatg gcatgtgtgc
     1741 catagcagac ctgggcctgg ctgtccgtca tgatgcagtc actgacacca ttgacattgc
     1801 cccgaatcag agggtgggga ccaaacgata catggcccct gaagtacttg atgaaaccat
     1861 taatatgaaa cactttgact cctttaaatg tgctgatatt tatgccctcg ggcttgtata
     1921 ttgggagatt gctcgaagat gcaattctgg aggagtccat gaagaatatc agctgccata
     1981 ttacgactta gtgccctctg acccttccat tgaggaaatg cgaaaggttg tatgtgatca
     2041 gaagctgcgt cccaacatcc ccaactggtg gcagagttat gaggcactgc gggtgatggg
     2101 gaagatgatg cgagagtgtt ggtatgccaa cggcgcagcc cgcctgacgg ccctgcgcat
     2161 caagaagacc ctctcccagc tcagcgtgca ggaagacgtg aagatctaat caactttctt
     2221 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2281 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2341 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2401 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     2461 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     2521 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     2581 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     2641 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     2701 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     2761 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     2821 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     2881 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     2941 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     3001 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     3061 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     3121 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     3181 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3241 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3301 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3361 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3421 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     3481 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     3541 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgactctaga
     3601 ggatccccgg gtaccgagct cgaattccgt gataggtggg ctgcccttcc tggttggctt
     3661 ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg gccagcctcg
     3721 cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca
     3781 cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca
     3841 aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata
     3901 taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa acggaattcg
     3961 agctcggtac ccggggatcc tctagagtcg agccacccat gaccaaaatc ccttaacgtg
     4021 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4081 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4141 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4201 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4441 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     4501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4621 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4681 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
