LOCUS       Exported                4931 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC126316*
               Fragment ends: L1fake and L2fake
               Fragment size: 1746
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..2413
                     /codon_start=1
                     /label=tev-Hs.AMHR2
                     /note="anti-Mullerian hormone receptor, type II"
                     /translation="ENLYFQGTMLGSLGLWALLPTAVEAPPNRRTCVFFEAPGVRGSTK
                     TLGELLDTGTELPRAIRCLYSRCCFGIWNLTQDRAQVEMQGCRDSDEPGCESLHCDPSP
                     RAHPSPGSTLFTCSCGTDFCNANYSHLPPPGSPGTPGSQGPQAAPGESIWMALVLLGLF
                     LLLLLLLGSIILALLQRKNYRVRGEPVPEPRPDSGRDWSVELQELPELCFSQVIREGGH
                     AVVWAGQLQGKLVAIKAFPPRSVAQFQAERALYELPGLQHDHIVRFITASRGGPGRLLS
                     GPLLVLELHPKGSLCHYLTQYTSDWGSSLRMALSLAQGLAFLHEERWQNGQYKPGIAHR
                     DLSSQNVLIREDGSCAIGDLGLALVLPGLTQPPAWTPTQPQGPAAIMEAGTQRYMAPEL
                     LDKTLDLQDWGMALRRADIYSLALLLWEILSRCPDLRPDSSPPPFQLAYEAELGNTPTS
                     DELWALAVQERRRPYIPSTWRCFATDPDGLRELLEDCWDADPEARLTAECVQQRLAALA
                     HPQESHPFPESCPRGCPPLCPEDCTSIPAPTILPCRPQRSACHFSVQQGPCSRNPQPAC
                     TLSPV"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2412..2511)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2529..2547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2552..2568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2999..3790
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3912..4021
                     /note="incP origin of transfer"
     rep_origin      4281..4869
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgctaggg tctttggggc tttgggcatt
      721 acttcccaca gctgtggaag cacccccaaa caggcgaacc tgtgtgttct ttgaggcccc
      781 tggagtgcgg ggaagcacaa agacactggg agagctgcta gatacaggca cagagctccc
      841 cagagctatc cgctgcctct acagccgctg ctgctttggg atctggaacc tgacccaaga
      901 ccgggcacag gtggaaatgc aaggatgccg agacagtgat gagccaggct gtgagtccct
      961 ccactgtgac ccaagtcccc gagcccaccc cagccctggc tccactctct tcacctgctc
     1021 ctgtggcact gacttctgca atgccaatta cagccatctg cctcctccag ggagccctgg
     1081 gactcctggc tcccagggtc cccaggctgc cccaggtgag tccatctgga tggcactggt
     1141 gctgctgggg ctgttcctcc tcctcctgct gctgctgggc agcatcatct tggccctgct
     1201 acagcgaaag aactacagag tgcgaggtga gccagtgcca gagccaaggc cagactcagg
     1261 cagggactgg agtgtggagc tgcaggagct gcctgagctg tgtttctccc aggtaatccg
     1321 ggaaggaggt catgcagtgg tttgggccgg gcagctgcaa ggaaaactgg ttgccatcaa
     1381 ggccttccca ccgaggtctg tggctcagtt ccaagctgag agagcattgt acgaacttcc
     1441 aggcctacag cacgaccaca ttgtccgatt tatcactgcc agccgggggg gtcctggccg
     1501 cctgctctct gggcccctgc tggtactgga actgcatccc aagggctccc tgtgccacta
     1561 cttgacccag tacaccagtg actggggaag ttccctgcgg atggcactgt ccctggccca
     1621 gggcctggca tttctccatg aggagcgctg gcagaatggc caatataaac caggtattgc
     1681 ccaccgagat ctgagcagcc agaatgtgct cattcgggaa gatggatcgt gtgccattgg
     1741 agacctgggc cttgccttgg tgctccctgg cctcactcag ccccctgcct ggacccctac
     1801 tcaaccacaa ggcccagctg ccatcatgga agctggcacc cagaggtaca tggcaccaga
     1861 gctcttggac aagactctgg acctacagga ttggggcatg gccctccgac gagctgatat
     1921 ttactctttg gctctgctcc tgtgggagat actgagccgc tgcccagatt tgaggcctga
     1981 cagcagtcca ccacccttcc aactggccta tgaggcagaa ctgggcaata cccctacctc
     2041 tgatgagcta tgggccttgg cagtgcagga gaggaggcgt ccctacatcc catccacctg
     2101 gcgctgcttt gccacagacc ctgatgggct gagggagctc ctagaagact gttgggatgc
     2161 agacccagaa gcacggctga cagctgagtg tgtacagcag cgcctggctg ccttggccca
     2221 tcctcaagag agccacccct ttccagagag ctgtccacgt ggctgcccac ctctctgccc
     2281 agaagactgt acttcaattc ctgcccctac catcctcccc tgtaggcctc agcggagtgc
     2341 ctgccacttc agcgttcagc aaggcccttg ttccaggaat cctcagcctg cctgtaccct
     2401 ttctcctgtg taatcaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     2461 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2521 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2581 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     2641 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     2701 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     2761 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2821 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     2881 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2941 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     3001 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     3061 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     3121 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     3181 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     3241 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     3301 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     3361 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     3421 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     3481 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     3541 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     3601 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     3661 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     3721 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     3781 cggcaaataa ccctcgactc tagaggatcc ccgggtaccg agctcgaatt ccgtgatagg
     3841 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     3901 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     3961 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     4021 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     4081 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     4141 ttatgcagcg gaaaacggaa ttcgagctcg gtacccgggg atcctctaga gtcgagccac
     4201 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     4261 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4321 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4381 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4441 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4501 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4561 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4621 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     4681 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4741 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4801 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4861 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4921 gctcacatgt t
//
