LOCUS       Exported                4721 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC063260*
               Fragment ends: L1fake and L2fake
               Fragment size: 1536
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..2203
                     /codon_start=1
                     /label=tev-Mm.Tgfbr1
                     /note="transforming growth factor, beta receptor I"
                     /translation="ENLYFQGTMEAAAAAPRRPQLLIVLVAAATLLPGAKALQCFCHLC
                     TKDNFTCETDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGAVTTTYC
                     CNQDHCNKIELPTTGPFSEKQSAGLGPVELAAVIAGPVCFVCIALMLMVYICHNRTVIH
                     HRVPNEEDPSLDRPFISEGTTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKG
                     RFGEVWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTW
                     TQLWLVSDYHEHGSLFDYLNRYTVTVEGMIKLALSTASGLAHLHMEIVGTQGKPAIAHR
                     DLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMK
                     HFESFKRADIYAMGLVFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKL
                     RPNIPNRWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEGIKM"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2202..2301)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2319..2337)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2342..2358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2789..3580
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3702..3811
                     /note="incP origin of transfer"
     rep_origin      4071..4659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catggaggcg gcggccgctg ctccacgtcg
      721 tccgcagctc ctcatcgtgt tggtggcggc ggcgacgctg ctcccggggg cgaaggcatt
      781 acagtgtttc tgccacctct gtacaaagga taattttacc tgtgagacag atggtctttg
      841 ctttgtctca gtcactgaga ccacagacaa agttatacac aatagtatgt gtatagctga
      901 aattgaccta attcctcgag acaggccatt tgtatgtgca ccatcttcaa aaacaggggc
      961 agttactaca acatattgct gcaatcagga ccactgcaat aaaatagaac tcccaactac
     1021 aggacctttt tcagaaaagc agtcagctgg ccttggtcct gtggagctgg cagctgtcat
     1081 tgctggtcca gtctgcttcg tctgcattgc acttatgctg atggtctata tctgccataa
     1141 ccgcactgtc attcaccacc gtgtgccaaa tgaagaggat ccatcactag atcgcccttt
     1201 catttcagag ggcaccacct taaaagattt aatttatgat atgacaacat cagggtctgg
     1261 atcaggttta ccactgcttg ttcaaagaac aattgccagg accattgtgt tacaagaaag
     1321 cattggcaaa ggtcggtttg gagaagtttg gcgaggcaaa tggcggggag aagaagttgc
     1381 tgtgaagata ttctcttcta gagaagagcg ttcatggttc cgagaggcag agatttatca
     1441 gactgtaatg ttacgccatg aaaatatcct gggatttata gcagcagaca acaaagacaa
     1501 tgggacatgg acgcagctgt ggttggtgtc agattatcat gagcatggat cccttttcga
     1561 ttacttgaat agatacactg ttactgtgga aggaatgatc aagcttgctc tgtccacagc
     1621 aagtggtctt gcccatcttc acatggagat tgttggtacc caaggaaaac cagctattgc
     1681 ccatagagat ttgaaatcaa agaatatctt ggtgaagaaa aatggaacct gttgtattgc
     1741 agacttggga cttgctgtga gacatgattc tgccacagat acaattgata ttgctccaaa
     1801 ccacagagta ggcactaaaa ggtacatggc ccctgaagtt ctagatgatt ccataaatat
     1861 gaaacatttt gaatccttca aacgcgctga catctatgca atgggcttag tgttctggga
     1921 aattgctcga cgctgttcta ttggtggaat ccatgaagac tatcagttgc cttattatga
     1981 tcttgtacct tctgatccat cggttgaaga aatgagaaaa gtagtttgcg aacagaagtt
     2041 aaggccaaat attccaaaca gatggcagag ctgtgaggcc ttgagagtga tggctaaaat
     2101 tatgagagaa tgctggtatg ccaatggagc agcaaggctg acagctttgc gaattaaaaa
     2161 aacattgtca caactcagcc aacaggaagg catcaaaatg taatcaactt tcttgtacaa
     2221 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     2281 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     2341 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     2401 acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac
     2461 ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac
     2521 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     2581 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     2641 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     2701 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     2761 cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac
     2821 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     2881 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     2941 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     3001 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     3061 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     3121 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     3181 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     3241 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     3301 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     3361 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     3421 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     3481 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     3541 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgactc tagaggatcc
     3601 ccgggtaccg agctcgaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
     3661 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     3721 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     3781 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     3841 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     3901 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttcgagctcg
     3961 gtacccgggg atcctctaga gtcgagccac ccatgaccaa aatcccttaa cgtgagttac
     4021 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4081 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4141 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4201 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4261 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4321 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4381 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4441 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4501 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4561 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4621 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4681 ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
