LOCUS       Exported                4496 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC119141*
               Fragment ends: L1fake and L2fake
               Fragment size: 1311
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..1978
                     /codon_start=1
                     /label=tev-Mm.Smad9
                     /note="MAD homolog 9 (Drosophila)"
                     /translation="ENLYFQGTMHPSTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKA
                     VDSLVKKLKKKKGAMDELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVW
                     RWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYRRVETPVLPPVLVPRHSEYNPQLS
                     LLAKFRSASLHSEPLMPHNATYPDSFQQSLCPAPPSSPGHVFPQSPCPTSYPHSPGSPS
                     DSPYQHSDFRPVCYEEPQHWCSVAYYELNNRVGETFQASSRSVLIDGFTDPSNNRNRFC
                     LGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECVSDSSIFVQSRNCNYQHGFHPA
                     TVCKIPSGCSLKVFNNQLFAQLLAQSVHHGFEVVYELTKMCTIRMSFVKGWGAEYHRQD
                     VTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(1977..2076)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2094..2112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2117..2133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2564..3355
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3477..3586
                     /note="incP origin of transfer"
     rep_origin      3846..4434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgcacccc agcaccccca tcagctccct
      721 cttctccttc accagccccg cagtgaagcg gctgctgggc tggaagcagg gagatgaaga
      781 ggagaagtgg gcagagaagg cggtggactc tttggtgaag aagttaaaga agaagaaagg
      841 cgccatggat gaactggaga gggcgctgag ctgcccgggt cagcctagca agtgtgtcac
      901 catcccacgg tccctcgatg gacgcctcca ggtgtcccac cgaaaggggc tgccccacgt
      961 catctactgc cgcgtgtggc gctggccaga cctgcagtcc catcatgagc tgaagccctt
     1021 ggagtgctgt gagttcccgt tcggctccaa gcagaaggag gtctgcatca acccatacca
     1081 ttaccgcaga gtggagaccc cagttctgcc tccagtgctg gtaccaagac acagcgagta
     1141 caaccctcag ctcagcctcc tggccaagtt ccgaagtgcc tcgctgcaca gcgaacccct
     1201 catgccgcac aacgccacct accctgactc tttccagcag tctctctgtc cggcaccgcc
     1261 ctcctcgcca ggccatgtgt ttccgcagtc tccatgcccc accagctacc cgcactcccc
     1321 cggaagtcct tcagacagtc cctatcaaca ctcagacttc cggccagttt gctacgagga
     1381 accccagcac tggtgttctg ttgcctacta cgaactaaac aaccgggtcg gagagacttt
     1441 ccaggcgtcc tcgcggagcg tgctcataga cggcttcacc gacccttcca ataacaggaa
     1501 taggttttgc cttgggcttc tctcaaatgt aaacagaaac tcgaccatag aaaacaccag
     1561 gaggcacatt ggaaagggtg tgcatttgta ctacgttggg ggcgaggtgt atgcggagtg
     1621 cgtgagcgac agcagcatct ttgtccagag ccggaactgc aactaccagc acggcttcca
     1681 cccggccacc gtctgcaaga tccccagcgg ctgcagcctc aaggtcttca acaaccagct
     1741 cttcgcccag ctgctcgccc agtccgtgca ccacggcttt gaagtggtgt atgagctgac
     1801 gaagatgtgc acgattcgga tgagctttgt gaagggctgg ggagcagagt atcatcgcca
     1861 ggatgtcacg agcaccccct gctggatcga gatccatctt catggaccgc tgcagtggtt
     1921 ggataaggtg ctcactcaga tgggctcccc acacaaccct atctcttcag tgtcttaatc
     1981 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2041 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2101 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2161 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     2221 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2281 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     2341 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     2401 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     2461 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2521 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     2581 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2641 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2701 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2761 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2821 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2881 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2941 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3001 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3061 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3121 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3181 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3241 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3301 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     3361 gactctagag gatccccggg taccgagctc gaattccgtg ataggtgggc tgcccttcct
     3421 ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg
     3481 ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa
     3541 gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg
     3601 gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa
     3661 ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa
     3721 cggaattcga gctcggtacc cggggatcct ctagagtcga gccacccatg accaaaatcc
     3781 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3841 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3901 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3961 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4021 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4081 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4141 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4201 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     4261 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4321 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4381 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4441 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
