LOCUS       Exported                4718 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC065106*
               Fragment ends: L1fake and L2fake
               Fragment size: 1533
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..2200
                     /codon_start=1
                     /label=tev-Mm.Bmpr1b
                     /note="Bmpr1b protein"
                     /translation="ENLYFQGTMLLRSSGKLNVGTKKEDGESTAPTPRPKILRCKCHHH
                     CPEDSVNNICSTDGYCFTMIEEDDSGMPVVTSGCLGLEGSDFQCRDTPIPHQRRSIECC
                     TERNECNKDLHPTLPPLKDRDFVDGPIHHKALLISVTVCSLLLVLIILFCYFRYKRQEA
                     RPRYSIGLEQDETYIPPGESLRDLIEQSQSSGSGSGLPLLVQRTIAKQIQMVKQIGKGR
                     YGEVWMGKWRGEKVAVKVFFTTEEASWFRETEIYQTVLMRHENILGFIAADIKGTGSWT
                     QLYLITDYHENGSLYDYLKSTTLDAKSMLKLAYSSVSGLCHLHTEIFSTQGKPAIAHRD
                     LKSKNILVKKNGTCCIADLGLAVKFISDTNEVDIPPNTRVGTKRYMPPEVLDESLNRNH
                     FQSYIMADMYSFGLILWEIARRCVSGGIVEEYQLPYHDLVPSDPSYEDMREIVCMKKLR
                     PSFPNRWSSDECLRQMGKLMTECWAQNPASRLTALRVKKTLAKMSESQDIKL"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2199..2298)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2316..2334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2339..2355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2786..3577
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3699..3808
                     /note="incP origin of transfer"
     rep_origin      4068..4656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgctctta cgaagctctg gaaaattaaa
      721 tgtgggcacc aagaaggagg atggagagag tacagccccc acccctcggc ccaagatcct
      781 acgttgtaaa tgccaccacc actgtccgga agactcagtc aacaatatct gcagcacaga
      841 tgggtactgc ttcacgatga tagaagaaga tgactctgga atgcctgttg tcacctctgg
      901 atgtctagga ctagaagggt cagattttca atgtcgtgac actcccattc ctcatcaaag
      961 aagatcaatt gaatgctgca cagaaaggaa tgagtgtaat aaagacctcc accccactct
     1021 gcctcctctc aaggacagag attttgttga tgggcccata caccacaagg ccttgcttat
     1081 ctctgtgact gtctgtagtt tactcttggt cctcattatt ttattctgtt acttcaggta
     1141 taaaagacaa gaagcccgac ctcggtacag cattgggctg gagcaggacg agacatacat
     1201 tcctcctgga gagtccctga gagacttgat cgagcagtct cagagctcgg gaagtggatc
     1261 aggcctccct ctgctggtcc aaaggacaat agctaagcaa attcagatgg tgaagcagat
     1321 tggaaaaggc cgctatggcg aggtgtggat gggaaagtgg cgtggagaaa aggtggctgt
     1381 gaaagtgttc ttcaccacgg aggaagccag ctggttccga gagactgaga tatatcagac
     1441 ggtcctgatg cggcatgaga atattctggg gttcattgct gcagatatca aagggactgg
     1501 gtcctggact cagttgtacc tcatcacaga ctatcatgaa aacggctccc tttatgacta
     1561 tctgaaatcc accaccttag acgcaaagtc catgctgaag ctagcctact cctctgtcag
     1621 cggcctatgc catttacaca cggaaatctt tagcactcaa ggcaagccag caatcgccca
     1681 tcgagacttg aaaagtaaaa acatcctggt gaagaaaaat ggaacttgct gcatagcaga
     1741 cctgggcttg gctgtcaagt tcattagtga cacaaatgag gttgacatcc cacccaacac
     1801 ccgggttggc accaagcgct atatgcctcc agaagtgctg gacgagagct tgaatagaaa
     1861 ccatttccag tcctacatta tggctgacat gtacagcttt ggactcatcc tctgggagat
     1921 tgcaaggaga tgtgtttctg gaggtatagt ggaagaatac cagcttccct atcacgacct
     1981 ggtgcccagt gacccttctt atgaggacat gagagaaatt gtgtgcatga agaagttacg
     2041 gccttcattc cccaatcgat ggagcagtga tgagtgtctc aggcagatgg ggaagcttat
     2101 gacagagtgc tgggcgcaga atcctgcctc caggctgacg gccctgagag ttaagaaaac
     2161 ccttgccaaa atgtcagagt cccaggacat taaactctaa tcaactttct tgtacaaagt
     2221 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2281 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2341 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2401 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     2461 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     2521 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     2581 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     2641 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     2701 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     2761 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     2821 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     2881 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     2941 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3001 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3061 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3121 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3181 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3241 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3301 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     3361 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     3421 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     3481 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     3541 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgactctag aggatccccg
     3601 ggtaccgagc tcgaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc
     3661 agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg
     3721 attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc
     3781 gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc
     3841 tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa
     3901 aatcgctata atgaccccga agcagggtta tgcagcggaa aacggaattc gagctcggta
     3961 cccggggatc ctctagagtc gagccaccca tgaccaaaat cccttaacgt gagttacgcg
     4021 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4201 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4441 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4681 cggttcctgg ccttttgctg gccttttgct cacatgtt
//
