LOCUS       Exported                4301 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC160360*
               Fragment ends: L1fake and L2fake
               Fragment size: 1116
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..1783
                     /codon_start=1
                     /label=tev-Mm.Acvr1c
                     /note="activin A receptor, type IC"
                     /translation="ENLYFQGTMLTNGKEQVIKSCVSLPELNAQVFCHSSNNVTKTECC
                     FTDFCNNITLHLPTGLPLLVQRTIARTIVLQEIVGKGRFGEVWHGRWCGEDVAVKIFSS
                     RDERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSEYHEQGSLYDYLNRN
                     IVTVAGMVKLALSIASGLAHLHMEIVGTQGKPAIAHRDIKSKNILVKKCDTCAIADLGL
                     AVKHDSIMNTIDIPQNPKVGTKRYMAPEMLDDTMNLSIFESFKRADIYSVGLVYWEIAR
                     RCSVGGVVEEYQLPYYDMVPSDPSIEEMRKVVCDQKLRPNLPNQWQSCEALRVMGRIMR
                     ECWYANGAARLTALRVKKTISQLCVKEDCKA"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(1782..1881)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1899..1917)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1922..1938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2369..3160
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3282..3391
                     /note="incP origin of transfer"
     rep_origin      3651..4239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgctaacc aacgggaaag agcaggtgat
      721 caaatcgtgt gtgtccctcc cggaactaaa tgctcaggtc ttctgtcaca gttccaacaa
      781 cgtgaccaaa accgaatgtt gcttcacaga cttctgcaac aatatcacac tgcaccttcc
      841 cacaggtctg cctctcttgg ttcaaagaac aatcgcaagg acaattgtac ttcaagaaat
      901 cgtaggaaaa ggtcggtttg gggaagtgtg gcacggaaga tggtgtggag aagatgtggc
      961 tgtgaaaata ttctcctcca gggatgagag atcttggttc cgtgaggcgg aaatttatca
     1021 gacggtgatg ctgagacacg agaacatcct cggtttcatc gcagctgaca acaaagataa
     1081 tggaacttgg acacaactct ggcttgtgtc agagtatcac gagcagggct ccttgtatga
     1141 ctatttgaac agaaacatag tgactgtggc tggaatggtc aagctggcgc tttccatagc
     1201 gagtggtctg gctcacctgc acatggagat cgtgggtact caaggtaagc ctgctattgc
     1261 tcaccgagat ataaagtcaa agaatatcct agtcaaaaaa tgtgacactt gtgccatagc
     1321 tgacttaggg ctggctgtga agcacgattc tatcatgaac actatagaca taccccagaa
     1381 tcctaaagtg ggaaccaaga ggtacatggc tcccgaaatg cttgatgata caatgaacct
     1441 gagcatcttt gagtccttca agcgagctga catctattcg gtggggctgg tttactggga
     1501 aatagctcga aggtgttcag ttggaggagt tgttgaggag taccagttgc cttactatga
     1561 catggtgcct tcagatcctt cgatagaaga aatgaggaag gttgtctgtg atcagaaact
     1621 ccgaccaaat ctcccaaacc agtggcaaag ctgtgaagcg ctgcgggtca tgggaagaat
     1681 aatgcgtgag tgctggtacg ccaacggggc agcacgcctg acagccctgc gcgtgaagaa
     1741 gaccatctcc cagctgtgtg tcaaggaaga ctgtaaagct taatcaactt tcttgtacaa
     1801 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1861 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     1921 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     1981 acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac
     2041 ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac
     2101 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     2161 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     2221 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     2281 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     2341 cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac
     2401 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     2461 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     2521 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     2581 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     2641 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     2701 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     2761 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     2821 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     2881 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     2941 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     3001 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     3061 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     3121 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgactc tagaggatcc
     3181 ccgggtaccg agctcgaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc
     3241 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     3301 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     3361 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     3421 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     3481 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttcgagctcg
     3541 gtacccgggg atcctctaga gtcgagccac ccatgaccaa aatcccttaa cgtgagttac
     3601 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3781 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4021 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4261 ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
