LOCUS       Exported                4748 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC106189*
               Fragment ends: L1fake and L2fake
               Fragment size: 1563
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..2230
                     /codon_start=1
                     /label=tev-Mm.Acvr2b
                     /note="Acvr2b protein"
                     /translation="ENLYFQGTMTAPWAALALLWGSLCAGSGRGEAETRECIYYNANWE
                     LERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEEN
                     PQVYFCCCEGNFCNERFTHLPEPGGPEVTYEPPPTAPTLLTVLAYSLLPIGGLSLIVLL
                     AFWMYRHRKPPYGHVDIHEDPGPPPPSPLVGLKPLQLLEIKARGRFGCVWKAQLMNDFV
                     AVKIFPLQDKQSWQSEREIFSTPGMKHENLLQFIAAEKRGSNLEVELWLITAFHDKGSL
                     TDYLKGNIITWNELCHVAETMSRGLSYLHEDVPWCRGEGHKPSIAHRDFKSKNVLLKSD
                     LTAVLADFGLAVRFEPGKPPGDTHGQVGTRRYMAPEVLEGAINFQRDAFLRIDMYAMGL
                     VLWELVSRCKAADGPVDEYMLPFEEEIGQHPSLEELQEVVVHKKMRPTIKDHWLKHPGL
                     AQLCVTIEECWDHDAEARLSAGCVEERVSLIRRSVNGTTSDCLVSLVTSVTNVDLLPKE
                     SSI"
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2229..2328)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2346..2364)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2369..2385)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2816..3607
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3729..3838
                     /note="incP origin of transfer"
     rep_origin      4098..4686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgacggcg ccctgggcgg ccctcgccct
      721 tctctgggga tcgctgtgcg ccggttccgg gcgaggggag gctgagactc gggagtgcat
      781 ctactacaac gccaactggg agctggagcg caccaaccag agcggcctgg agcgctgcga
      841 gggggaacag gacaagcggc tgcactgcta cgcctcgtgg cgcaacagct cgggcaccat
      901 cgagctggtg aagaagggct gctggctaga tgacttcaat tgctacgaca ggcaggagtg
      961 tgtggccacc gaggagaacc cccaggtgta cttctgctgc tgcgaaggca acttctgcaa
     1021 cgagcgcttc acccacttgc cggagcctgg gggcccagaa gtcacgtacg agccaccccc
     1081 gacagccccc accctgctca cggtgctggc ctactcgctg ctgcccattg gaggcctctc
     1141 tctcatcgtc ctgctggcct tctggatgta tcgtcatcgg aaacctccct acggccatgt
     1201 ggacatccat gaggacccgg ggcctccgcc cccatcccct ctggtgggcc tgaagccact
     1261 acagctgctg gagatcaagg ctcggggccg ctttggctgc gtttggaagg ctcagctcat
     1321 gaacgacttt gtggctgtga agatcttccc acttcaggac aagcagtcgt ggcagagtga
     1381 acgggaaatc ttcagcacac ccggcatgaa gcacgaaaac ttgttgcagt tcattgctgc
     1441 cgagaaacga ggctccaacc tggaggtgga gctgtggctc atcacagcct tccacgacaa
     1501 gggctccctc acggattacc tcaaggggaa catcatcacg tggaacgaac tgtgccacgt
     1561 ggcggagacg atgtcacgag gcctctcata cctgcatgag gatgtgccgt ggtgtcgtgg
     1621 tgagggccac aagccttcta ttgcccacag ggacttcaaa agcaagaatg tactgctgaa
     1681 gagcgacctc accgcggtgc tggctgactt cggcctggct gttcggtttg agccagggaa
     1741 gcctcctggg gatacccatg gacaggttgg caccagacgg tacatggccc ctgaggtgct
     1801 ggaaggagcc atcaacttcc agagagacgc cttcctgcgt atcgacatgt acgccatggg
     1861 cctggtgctg tgggagctcg tctctcggtg caaggctgca gacgggcctg tcgatgagta
     1921 catgctgccc ttcgaggagg agattggcca gcacccttcg ctggaggagc ttcaggaggt
     1981 ggttgtccac aagaagatga ggcccacgat taaggatcac tggctgaaac acccgggcct
     2041 ggcccagctc tgcgtgacca tcgaggagtg ctgggaccat gatgcagagg ctcgcctttc
     2101 tgcaggctgt gtagaagagc gggtatccct gatcaggagg tcggtcaacg gcactacctc
     2161 ggactgtctc gtctctctgg tgacctccgt caccaatgtg gacctgctcc ctaaagagtc
     2221 cagcatctaa tcaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2281 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2341 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2401 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     2461 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     2521 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     2581 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2641 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     2701 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     2761 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     2821 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     2881 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     2941 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     3001 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     3061 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3121 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3181 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3241 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3301 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3361 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3421 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3481 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3541 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     3601 caaataaccc tcgactctag aggatccccg ggtaccgagc tcgaattccg tgataggtgg
     3661 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
     3721 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
     3781 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
     3841 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
     3901 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
     3961 tgcagcggaa aacggaattc gagctcggta cccggggatc ctctagagtc gagccaccca
     4021 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     4081 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4141 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4201 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4261 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4321 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4381 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4441 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     4501 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4561 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4621 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4681 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4741 cacatgtt
//
