LOCUS       Exported                4916 bp ds-DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Vector molecule:  253 with fake Ls
               Fragment ends: L2fake and L1fake
               Fragment size: 3185
            Insert molecule:  BC119571*
               Fragment ends: L1fake and L2fake
               Fragment size: 1731
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..2398
                     /codon_start=1
                     /label=tev-Mm.Amhr2
                     /note="anti-Mullerian hormone type 2 receptor"
                     /translation="ENLYFQGTMLGTLGLWTLLPAAAQVSPNRRTCVFFEAPGVRGSTK
                     TLGEMVDAGPGPPKGIRCLYSHCCFGIWNLTHGRAQVEMQGCRDSDEPGCESLHCDPVP
                     RAHPNPSSTLFTCSCGTDFCNANYSHLPPSGNQGAPGPQEPQATPGGPVWMALLLLGMF
                     LVLLLSSIILALLQRKACRVQGGSDPEPGSGGDCSEELPELAELRFSQVIQEGGHAVVW
                     AGRLQGEMVAIKAFPPRAVAQFRAERAVYQLLGLQHDHIVRFITAGQGGPGPLPSGPLL
                     VLELYPKGSLCHYLTQYTSDWGSSLRMALSLAEGLAFLHEERWQDGQYKPGIAHRDLSS
                     QNVLIREDRSCAIGDLGLALVLPGLAQPPALAPTQPRGPAAILEAGTQRYMAPELLDKT
                     LDLQDWGTALQRADVYSLALLLWEILSRCSDLRPDHRPPPFQLAYEAELGSNPSACELW
                     ALAVEERKRPNIPSTWSCSATDPRGLRELLEDCWDADPEARLTAECVQQRLAALAYPHG
                     ASSFPESPQGCPENCLSAPASAVFPCRPQQSSCLLSVQQGPGSRSPDPVGDTVQVYVNE
                     "
     CDS             668..688
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2397..2496)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2514..2532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2537..2553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2984..3775
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            3897..4006
                     /note="incP origin of transfer"
     rep_origin      4266..4854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcgaa aacctgtact tccaaggcac catgctgggg acactggggc tttggacact
      721 gcttcctgcg gcagcacaag tatccccaaa caggaggacc tgtgtgttct ttgaggctcc
      781 tggagtgcgg ggaagcacaa agacactggg ggaaatggta gatgcaggac caggcccccc
      841 caagggtatc cgctgcctct acagccattg ctgctttggg atctggaatc ttacccatgg
      901 ccgggcacaa gtggagatgc aaggatgccg agacagtgat gagccaggct gtgagtccct
      961 ccactgtgac ccagtcccca gagcccaccc gaaccccagc tccacactct tcacgtgttc
     1021 ctgtggcact gacttctgca atgccaatta cagccatctg cctccttcgg gaaaccaagg
     1081 ggctcctggc ccccaggagc cccaggctac cccaggtggg cccgtctgga tggcactgct
     1141 gctcctggga atgtttctcg tgttgttgct gagtagcatc atcttggccc tgctacaacg
     1201 aaaggcctgc agagtgcaag gtgggtcaga cccagagccc ggctcaggcg gagactgcag
     1261 tgaggaactg ccagagctgg ccgagctgcg tttctcccag gtaatccagg aaggaggtca
     1321 tgcagtcgtc tgggccggga ggcttcaggg tgagatggta gccatcaagg ccttcccccc
     1381 aagggctgtg gcccagttcc gagctgagag ggctgtgtac cagctgctag gcctacagca
     1441 tgaccatatc gttcgcttta tcactgctgg ccagggaggc cctggccctc tgccctctgg
     1501 gcctttgctg gtactggaac tgtaccctaa gggctcctta tgccactact tgactcagta
     1561 cacgagcgac tggggaagtt ccttgaggat ggctctgtcc ctggcagagg gcttggcttt
     1621 tctccacgag gagcgttggc aggatggcca gtacaaacca ggtatcgccc accgagacct
     1681 gagcagccag aatgtgctca ttcgggaaga taggtcatgc gccatcgggg acctgggcct
     1741 tgccttggta ctccctggtc ttgctcagcc ccctgccttg gcccctactc aaccccgagg
     1801 ccccgctgcc attttggagg ctggcaccca gcggtacatg gctccagagc tcttggacaa
     1861 gactctagac ttgcaggact ggggtacagc tctccagaga gcggatgttt actctctggc
     1921 gctactactg tgggagatcc tgagccgctg ttccgatttg aggcctgacc acagaccacc
     1981 acctttccaa ttggcttatg aggcagaact gggcagcaat cccagtgcct gtgagctctg
     2041 ggccctggca gtggaggaga ggaagcgccc caacatccca tccacttgga gctgctctgc
     2101 cacagaccct cgggggctga gggagctcct ggaagattgc tgggacgcag acccagaagc
     2161 acggctgaca gctgagtgtg tgcagcagcg cctggcagcc ctggcttacc ctcacggggc
     2221 gtcctctttc ccagagagtc cacagggctg cccagagaac tgcctttcag ctcccgcctc
     2281 tgccgttttc ccctgtcggc ctcagcagag ttcttgtctc ctcagtgttc agcaaggccc
     2341 tggctctagg agtcccgatc ctgtaggcga cactgttcag gtgtatgtaa atgagtaatc
     2401 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2461 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2521 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2581 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     2641 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2701 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     2761 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     2821 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     2881 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2941 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     3001 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     3061 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     3121 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     3181 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     3241 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     3301 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     3361 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3421 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3481 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3541 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3601 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3661 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3721 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     3781 gactctagag gatccccggg taccgagctc gaattccgtg ataggtgggc tgcccttcct
     3841 ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg
     3901 ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa
     3961 gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg
     4021 gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa
     4081 ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa
     4141 cggaattcga gctcggtacc cggggatcct ctagagtcga gccacccatg accaaaatcc
     4201 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4261 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4321 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4381 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4441 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4501 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4561 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4621 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     4681 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4741 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4801 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4861 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
