LOCUS       2-miniFXN8              5902 bp    DNA     circular SYN 11-DEC-2024
DEFINITION  2-miniFXN8.
ACCESSION   2-miniFXN8
VERSION     2-miniFXN8
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             3875..4669
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(2766..2900)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(1044..1178)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(4919..5779)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             3223..3526
                     /note="pLannotate"
                     /label="ccdB (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.3"
                     /fragment="True"
                     /other="CDS"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(3045..3063)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(1336..2319)
                     /note="pLannotate"
                     /label="FXN"
                     /database="swissprot"
                     /identity="64.0"
                     /match_length="156.2"
                     /fragment="False"
                     /other="CDS"
     CDS             3066..3213
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="85.1"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(5780..5832)
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="82.8"
                     /fragment="True"
                     /other="promoter"
     promoter        4767..4826
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="58.3"
                     /fragment="True"
                     /other="promoter"
     CDS             3574..3843
                     /note="pLannotate"
                     /label="tnpA (fragment)"
                     /database="swissprot"
                     /identity="97.8"
                     /match_length="18.9"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(4690..4740)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.2"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1297..1320)
                     /note="pLannotate"
                     /label="3xFLAG (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2923..3006)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1201..1284)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagagtc gcagatccag acatgataag atacattgat gagtttggac
     1081 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1141 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1201 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1261 aatgtggtat ggctgattat gatcctctag atatcactta tcgtcgtcat ccttgtaatc
     1321 catcaccagt atccgagcat cttttccgga ataggccaag gaagacaagt ccagtttggt
     1381 ttttaaggct ttagtgagct ctgcggccag cagctcatgg agggacacgc cgtcgtggga
     1441 gtacacccag tttttcccag tccagtcata acgcttaggt ccactggatg gagaagatag
     1501 ccagatttgc ttgtttggcg tctgcttgtt gatcacatag gttcctagat ctccacccag
     1561 tttgacagtt aagacaccac tcccaaagga gacatcatag tcctcaaacg tgtatggctt
     1621 gtctgcaagg tcttcaaaaa actctgctaa agagtccagc gtttcctctg ctagtctttc
     1681 ataggtggtc tcatctagag agcctgggtg gcccaaagtt ccagatttcc tcaaattcat
     1741 caaatagaca ctctgctttt tgacattcca aatctggttg aggccacgtt ggttcgaact
     1801 ctgaaagcca aagttaagaa gtacgttact atttttcagt gaattataac ccattaaccg
     1861 gtcccctcca ccagcctgcc ctgccttcgc ccgtgccttg acccgcagtc gcaccgcagg
     1921 acaaaatgtc cccttttcct tcggaaagca gaagccagtg taaatgcaac cgggagaacc
     1981 agagaaggga gttgcaaggc cgcttccgcc gggccgccct gagaaggagc ggggtgagct
     2041 agtccagcgc gcgtacccgg agcgaccccg gcgtgcgcgg cgcctccctg tgcaggcgtg
     2101 cggcgtgcgg cccgcggcgt gcggcccgcg gctgttcccg gcgcggatac ttactgcgcg
     2161 gcggggcgtg caggtcgcat cgatgtcggt gcgcaggcca cggcggccgc agagtggggc
     2221 caactctgcc ggccgcggga cccgggtgag ggtctgggcc tgggccgggc tgggtgacgc
     2281 caggaggccg gctactgcgc ggcgcccgag agtccacatg ctgctccggg tctgccgccc
     2341 gctccgccct ccagcgctgg gtgctgcggc gacccctggt ggccactggc cgcaggcact
     2401 cttctgtggg ggagcagcta gaggttagac ctcaggaaga acttcccagc ttagcactat
     2461 tcgtgcattt aacaaaaatg gagagcctgc tttgtgcaaa gcacggagtg caaccaggac
     2521 ccctgaccca agggagactg cagcctggtg gcccgccgct tccaaaattc taaacaggag
     2581 gaacttggga gctgctgtct tgctgggaag tgggtgcatg cacaaattga ggctgcttgg
     2641 ccgccggtat gggtttacag cagttgggta tgtggggcca ggagacggat gccttgtgta
     2701 aatctgtact ctgcccactt cctagctgtg tgaccagagg gcaagttaaa ttcactagag
     2761 tcgcagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2821 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2881 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2941 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     3001 atgatcctct agttacgacg tcgcggccgc taaattcaat tcgccctata gtgagtcgta
     3061 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     3121 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     3181 caccgatcgc ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac
     3241 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     3301 gccggggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     3361 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     3421 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     3481 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggcatgag
     3541 attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg
     3601 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
     3661 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
     3721 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
     3781 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag
     3841 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     3901 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     3961 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     4021 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     4081 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     4141 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     4201 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     4261 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     4321 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     4381 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     4441 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     4501 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     4561 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     4621 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttattaacgc
     4681 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4741 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4801 attcaaatat gtatccgctc atgagattat caaaaaggat cttcacctag atccttttaa
     4861 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5821 attgtctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5881 tagaaaagat caaaggatct tc
//
