LOCUS       Exported                6838 bp ds-DNA     circular SYN 30-MAR-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 8, 2025 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          694..2940
                     /organism="pDisplay-BirA-ER"
                     /mol_type="other DNA"
     source          1327..1329
                     /organism="pDisplay-BirA-ER"
                     /mol_type="other DNA"
     unsure          1..141
                     /label=L-ITR
                     /note="/vntifkey=21"
     primer_bind     26..45
                     /label=PAAV-HaloDA1.0-mut-seq-primer1-fwd
     promoter        156..597
                     /label=synapsin
     primer_bind     complement(338..354)
                     /label=ADRA2A-insert-R249-rev
     misc_feature    446..469
                     /label=ColonyF_hSyn
     primer_bind     568..619
                     /label=PAAV-hSyn-HaloDA1.0-mut-fwd
     misc_feature    568..597
                     /label=138_F_hSyn_GRAB
     primer_bind     578..597
                     /label=PAAV-HaloDA1.0-mut-seq-primer2-fwd
     CDS             598..2940
                     /codon_start=1
                     /label=ORF
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGRTLNTSAMDGTGL
                     VVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLV
                     AVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPFRYE
                     RKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLS
                     RTYAISASVIPFYIPVAIMIVCYTRIYRIAKRRTRVPPSRRGPDAVAAPPGGTERRPNG
                     MYLPEMCRETFQAYRTTDVGRKLIITKNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPV
                     DREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLA
                     KSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGGGTGGSGGTGGSGGTGGS
                     MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTH
                     RCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKR
                     NPERVKGIAFMEFIRPIPTWDDWPEFANWKTLSVIMGVFVCCWLPFFILNCIRPFCGSG
                     WTQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPATNNAIET
                     VSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPA
                     LSVILDYDTDVSLEKIQPITQNGQHPT"
     misc_feature    598..660
                     /label=Ig K leader sequence
                     /label=Ig K leader sequence
     primer_bind     661..717
                     /label=pdisplay-BsmBI-D1-fwd
     misc_feature    663..687
                     /label=overlap-pdisplayJM IgK
                     /label=overlap-pdisplayJM IgK
     misc_feature    688..693
                     /label=TG
     CDS             694..2940
                     /codon_start=1
                     /label=D1
                     /label=D1 cpEGFP
                     /translation="RTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVCA
                     AVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIM
                     CSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSWH
                     KAKPTSPSDGNATSLAETIDNCDSSLSRTYAISASVIPFYIPVAIMIVCYTRIYRIAKR
                     RTRVPPSRRGPDAVAAPPGGTERRPNGMYLPEMCRETFQAYRTTDVGRKLIITKNVFIE
                     GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWL
                     HQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIAR
                     WLSTLEISGGGTGGSGGTGGSGGTGGSMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDG
                     TPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAF
                     IEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDDWPEFANWKTL
                     SVIMGVFVCCWLPFFILNCIRPFCGSGWTQPFCIDSNTFDVFVWFGWANSSLNPIIYAF
                     NADFRKAFSTLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIP
                     HAVGSSEDLKKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT"
     CDS             754..756
                     /label=V1.31
     CDS             907..909
                     /label=V2.53
     CDS             1075..1077
                     /label=F34.51
     misc_feature    1282..1284
                     /label=S222(5.42)A
     misc_feature    1294..1296
                     /label=S226(5.46)P
     misc_feature    1327..1329
                     /label=T5.57C
     primer_bind     complement(1335..1352)
                     /label=D1-insert-N1-rev
     primer_bind     complement(1338..1352)
                     /label=D1-insert-5.69I-rev
     CDS             1351..2409
                     /label=ADRA2A
     primer_bind     1351..1372
                     /label=N3-fwd
     primer_bind     1351..1367
                     /label=N2-fwd
     misc_feature    1354..1356
                     /label=5.66
     primer_bind     complement(1357..1386)
                     /label=Primer 1
     primer_bind     1357..1379
                     /label=N4-fwd
     misc_feature    1357..1359
                     /label=5.67
     gene            1360..1440
                     /label=Loop 3
                     /label=Loop 3
     misc_feature    1360..1362
                     /label=5.68
     primer_bind     complement(1362..1383)
                     /label=ADRA2A-insert-5.75R-rev
     primer_bind     1363..1384
                     /label=N5-fwd
     misc_feature    1363..1365
                     /label=5.69
     primer_bind     complement(1366..1386)
                     /label=ADRA2A-insert-R249-rev
     misc_feature    1366..1368
                     /label=5.70
     primer_bind     complement(1368..1389)
                     /label=ADRA2A-insert-G250-rev
     primer_bind     1369..1390
                     /label=N6-fwd
     misc_feature    1369..1371
                     /label=5.71
     misc_feature    1372..1374
                     /label=1N-R
     primer_bind     1375..1398
                     /label=N7-fwd
     misc_feature    1375..1377
                     /label=5.73
     misc_feature    1378..1380
                     /label=5.74
     primer_bind     1381..1403
                     /label=N8-fwd
     primer_bind     1381..1399
                     /label=OVERLAP-REV
     misc_feature    1381..1383
                     /label=5.75
     misc_feature    1384..1440
                     /label=ICL3
     primer_bind     complement(1384..1413)
                     /label=OVERLAP-REV
     primer_bind     1384..1408
                     /label=LOOP-FWD
     misc_feature    1384..1386
                     /label=R249
     primer_bind     1387..1409
                     /label=N9-fwd
     misc_feature    1387..1389
                     /label=G250
     misc_feature    1390..1392
                     /label=P251
     misc_feature    1393..1395
                     /label=D252
     misc_feature    1396..1398
                     /label=A253
     primer_bind     1399..1421
                     /label=N10-fwd
     misc_feature    1399..1401
                     /label=2N
     misc_feature    1408..1410
                     /label=P257
     misc_feature    1420..1422
                     /label=3N
     primer_bind     complement(1428..1440)
                     /label=ADR-cpHT-NNTrun5-fwd
     misc_feature    1429..1431
                     /label=R264
     primer_bind     complement(1430..1440)
                     /label=ADR-cpHT-NNTrun4-P-fwd
     misc_feature    1438..1440
                     /label=G267
     misc_feature    1441..1443
                     /label=L5.95M
     misc_feature    1444..1446
                     /label=T5.96Y
     CDS             1447..1449
                     /codon_start=1
                     /label=P270L or P5.97
                     /translation="L"
     primer_bind     1450..1474
                     /label=cpHalotag-fwd
     primer_bind     1450..1474
                     /label=cpHalotag-N-all(P)-fwd
     CDS             1450..1464
                     /codon_start=1
                     /label=linker
                     /translation="PEMCR"
     primer_bind     1461..1478
                     /label=cpHalotag-Ntrun2-F-fwd
     primer_bind     1461..1476
                     /label=cpHalotag-Ntrun1-E-fwd
     CDS             1465..1917
                     /codon_start=1
                     /label=C
                     /note="/region_type=Domain"
                     /translation="ETFQAYRTTDVGRKLIITKNVFIEGTLPMGVVRPLTEVEMDHYRE
                     PFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
                     EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISG"
     primer_bind     complement(1465..1494)
                     /label=ADR-overlap-rev
     primer_bind     1465..1488
                     /label=cpHalotag-Ntrun5-fwd
     primer_bind     1465..1487
                     /label=cpHalotag-no linker-fwd
     misc_feature    1480..1482
                     /label=F6Y
     misc_feature    1489..1491
                     /label=9
     misc_feature    1504..1506
                     /label=14
     CDS             1516..1518
                     /label=T18E
     CDS             1519..1521
                     /label=Q19K
     misc_feature    1522..1524
                     /label=20
     misc_feature    1543..1545
                     /label=27
     misc_feature    1546..1548
                     /label=28
     misc_feature    1549..1551
                     /label=29
     primer_bind     complement(1669..1692)
                     /label=cphalotag-conoly-rev
     primer_bind     complement(1721..1741)
                     /label=cpHalotag-seq-rev
     CDS             1918..1971
                     /codon_start=1
                     /label=Circular permutation linker
                     /label=Circular permutation linker
                     /translation="GGTGGSGGTGGSGGTGGS"
     CDS             1972..2406
                     /codon_start=1
                     /label=N
                     /note="/region_type=Domain"
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDDWPEFA"
     region          2143..2145
                     /label=mutation
     primer_bind     2364..2394
                     /label=overlap-fwd
     primer_bind     complement(2369..2394)
                     /label=cpHalotag-no linker-rev
     primer_bind     complement(2372..2394)
                     /label=cpHalotag-Ctrun5-rev
     primer_bind     complement(2377..2400)
                     /label=cpHalotag-Ctrun3-E-rev
     primer_bind     complement(2377..2397)
                     /label=cpHalotag-Ctrun4-P-rev
     primer_bind     complement(2381..2403)
                     /label=cpHalotag-Ctrun2-F-rev
     primer_bind     complement(2384..2406)
                     /label=cpHalotag-Ctrun1-A-rev
     primer_bind     complement(2386..2407)
                     /label=loop-rev-2
     primer_bind     complement(2386..2406)
                     /label=HaloDA0.6-rev
     primer_bind     complement(2387..2406)
                     /label=cpHalotag-rev
     primer_bind     complement(2387..2406)
                     /label=cpHalotag-C-all(P)-rev
     region          2395..2406
                     /label=linker
     misc_feature    2407..2409
                     /label=C5-N
     misc_feature    2410..2412
                     /label=G6.34W
     primer_bind     2412..2434
                     /label=D1-insert-6.34L-fwd
     primer_bind     2412..2430
                     /label=D1-insert-6.33V-fwd
     primer_bind     2412..2425
                     /label=D1-insert-C1-fwd
     primer_bind     2412..2424
                     /label=D1-insert-6.31T-fwd
     primer_bind     2413..2435
                     /label=D1-insert-6.35K-fwd
     misc_feature    2413..2415
                     /label=6.35K
     CDS             2416..2418
                     /label=T6.36
     CDS             2428..2430
                     /label=I6.40
     CDS             2461..2463
                     /label=6.51
     misc_feature    2482..2484
                     /label=L6.58R
     misc_feature    2503..2505
                     /label=E302W
     CDS             2548..2550
                     /label=7.39
     primer_bind     complement(2917..2940)
                     /label=pdisplay-NheI-D1-rev
     primer_bind     complement(2921..2970)
                     /label=PAAV-hSyn-HaloDA1.0-mut-rev
     unsure          2955..3563
                     /label=WPRE
                     /note="/vntifkey=21"
     misc_feature    complement(3040..3057)
                     /label=pWPTRev
     polyA_signal    3583..4061
                     /label=hGH polyA
                     /label=hGH polyA
                     /label=PolyA signal
                     /note="/vntifkey=25"
     unsure          4101..4241
                     /label=R-ITR
                     /note="/vntifkey=21"
     primer_bind     complement(4197..4216)
                     /label=PAAV-HaloDA1.0-mut-seq-primer1-fwd
     rep_origin      4333..4639
                     /label=f1 Origin
                     /label=f1 Origin
                     /label=Replication origin
                     /note="/vntifkey=33"
     unsure          5158..6015
                     /label=AmpR
                     /note="/vntifkey=21"
     rep_origin      6166..6833
                     /label=pUC ori
                     /label=pUC ori
                     /label=Replication origin
                     /note="/vntifkey=33"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg ggtgcctacc
      181 tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc
      241 ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt
      301 cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca
      361 ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg
      421 gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg
      481 ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg
      541 tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg tgccaccatg
      601 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      661 acaagtttgt acaaaaaagt tggcaccacc ggtaggactc tgaacacctc tgccatggac
      721 gggactgggc tggtggtgga gagggacttc tctgttcgta tcctcactgc ctgtttcctg
      781 tcgctgctca tcctgtccac gctcctgggg aacacgctgg tctgtgctgc cgttatcagg
      841 ttccgacacc tgcggtccaa ggtgaccaac ttctttgtca tctccttggc tgtgtcagat
      901 ctcttggtgg ccgtcctggt catgccctgg aaggcagtgg ctgagattgc tggcttctgg
      961 ccctttgggt ccttctgtaa catctgggtg gcctttgaca tcatgtgctc cactgcatcc
     1021 atcctcaacc tctgtgtgat cagcgtggac aggtattggg ctatctccag ccctttccgg
     1081 tatgagagaa agatgacccc caaggcagcc ttcatcctga tcagtgtggc atggaccttg
     1141 tctgtactca tctccttcat cccagtgcag ctcagctggc acaaggcaaa acccacaagc
     1201 ccctctgatg gaaatgccac ttccctggct gagaccatag acaactgtga ctccagcctc
     1261 agcaggacat atgccatctc agcctctgta ataccctttt acatccctgt ggccatcatg
     1321 attgtctgct acaccaggat ctacaggatt gccaagcgtc gcacccgcgt gccacccagc
     1381 cgccggggtc cggacgccgt cgccgcgccg ccggggggca ccgagcgcag gcccaacggt
     1441 atgtacttgc cagagatgtg ccgtgagacc ttccaggcct accgcaccac cgacgtcggc
     1501 cgcaagctga tcatcaccaa gaacgttttt atcgagggta cgctgccgat gggtgtcgtc
     1561 cgcccgctga ctgaagtcga gatggaccat taccgcgagc cgttcctgaa tcctgttgac
     1621 cgcgagccac tgtggcgctt cccaaacgag ctgccaatcg ccggtgagcc agcgaacatc
     1681 gtcgcgctgg tcgaagaata catggactgg ctgcaccagt cccctgtccc gaagctgctg
     1741 ttctggggca ccccaggcgt tctgatccca ccggccgaag ccgctcgcct ggccaaaagc
     1801 ctgcctaact gcaaggctgt ggacatcggc ccgggtctga atctgctgca agaagacaac
     1861 ccggacctga tcggcagcga gatcgcgcgc tggctgtcga cgctcgagat ttccggcgga
     1921 ggaacaggtg gttctggtgg aacagggggt agcggaggta caggaggaag tatggcggag
     1981 atcggaactg gattcccgtt tgatccgcat tatgtggaag ttctgggaga gcgcatgcat
     2041 tatgtggacg ttggtcctcg tgatgggaca ccagtgctgt tccttcacgg caatccgaca
     2101 tcgtcgtacg tgtggcgtaa tatcatcccg cacgttgccc ccacgcaccg ctgcattgcc
     2161 cctgacttaa ttggtatggg gaaaagtgat aagcctgatc tggggtactt ctttgacgac
     2221 cacgtacgct tcatggatgc ttttattgaa gcattgggtt tggaggaagt agttttggtg
     2281 atccatgatt ggggtagtgc tctggggttc cattgggcca agcgtaaccc agaacgcgtg
     2341 aaaggaattg cctttatgga gttcatccgt ccgattccaa catgggacga ctggcctgag
     2401 tttgcaaact ggaagactct gtcggtgatc atgggtgtgt ttgtgtgctg ttggctacct
     2461 ttcttcatct tgaactgcat tcggcccttc tgtgggtctg ggtggacgca gcccttctgc
     2521 attgattcca acacctttga cgtgtttgtg tggtttgggt gggctaattc atccttgaac
     2581 cccatcattt atgcctttaa tgctgatttt cggaaggcat tttcaaccct cttaggatgc
     2641 tacagacttt gccctgcgac gaataatgcc atagagacgg tgagtatcaa taacaatggg
     2701 gccgcgatgt tttccagcca tcatgagcca cgaggctcca tctccaagga gtgcaatctg
     2761 gtttacctga tcccacatgc tgtgggctcc tctgaggacc tgaaaaagga ggaggcagct
     2821 ggcatcgcca gacccttgga gaagctgtcc ccagccctat cggtcatatt ggactatgac
     2881 actgacgtct ctctggagaa gatccaaccc atcacacaaa acggtcagca cccaacctga
     2941 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     3001 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3061 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3121 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3181 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3241 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3301 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3361 tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc
     3421 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3481 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3541 gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg
     3601 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     3661 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     3721 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     3781 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     3841 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     3901 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     3961 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     4021 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca
     4081 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     4141 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     4201 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     4261 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     4321 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4381 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4441 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4501 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     4561 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4621 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     4681 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4741 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     4801 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4861 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4921 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     4981 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     5041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5101 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5161 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5221 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5281 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5341 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5401 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5461 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5521 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5581 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5641 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5701 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5761 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5821 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5881 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5941 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6001 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6061 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6241 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6301 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     6361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6541 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6601 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6661 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6721 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6781 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
