LOCUS       Exported                8901 bp ds-DNA     circular UNA 08-FEB-2025
DEFINITION  natural circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 8, 2025 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          833..3079
                     /organism="pDisplay-BirA-ER"
                     /mol_type="other DNA"
     source          1421..1435
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1421..1435
                     /organism="pDisplay-BirA-ER"
                     /mol_type="other DNA"
     source          3680..4393
                     /organism="pDisplay-BirA-ER"
                     /mol_type="other DNA"
     promoter        11..627
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter
     misc_feature    90..377
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
                     /label=CAG_enhancer
     promoter        638..656
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
                     /label=T7_promoter
     CDS             737..3079
                     /codon_start=1
                     /label=HaloDA1.0
                     /label=ORF
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGRTLNTSAMDGTGL
                     VVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLV
                     AVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPFRYE
                     RKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLS
                     RTYAISASVIPFYIPVAIMIVCYTRIYRIAKRRTRVPPSRRGPDAVAAPPGGTERRPNG
                     MYLPEMCRETFQAYRTTDVGRKLIITKNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPV
                     DREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLA
                     KSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGGGTGGSGGTGGSGGTGGS
                     MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTH
                     RCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKR
                     NPERVKGIAFMEFIRPIPTWDDWPEFANWKTLSVIMGVFVCCWLPFFILNCIRPFCGSG
                     WTQPFCIDSNTFDVFVWFGWANSSLNPIIYAFNADFRKAFSTLLGCYRLCPATNNAIET
                     VSINNNGAAMFSSHHEPRGSISKECNLVYLIPHAVGSSEDLKKEEAAGIARPLEKLSPA
                     LSVILDYDTDVSLEKIQPITQNGQHPT"
     misc_feature    737..799
                     /label=Ig K leader sequence
                     /label=Ig K leader sequence
     primer_bind     800..856
                     /label=pdisplay-BsmBI-D1-fwd
     misc_feature    802..826
                     /label=overlap-pdisplayJM IgK
                     /label=overlap-pdisplayJM IgK
     misc_feature    827..832
                     /label=TG
     CDS             833..3079
                     /codon_start=1
                     /label=D1
                     /label=D1 cpEGFP
                     /translation="RTLNTSAMDGTGLVVERDFSVRILTACFLSLLILSTLLGNTLVCA
                     AVIRFRHLRSKVTNFFVISLAVSDLLVAVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIM
                     CSTASILNLCVISVDRYWAISSPFRYERKMTPKAAFILISVAWTLSVLISFIPVQLSWH
                     KAKPTSPSDGNATSLAETIDNCDSSLSRTYAISASVIPFYIPVAIMIVCYTRIYRIAKR
                     RTRVPPSRRGPDAVAAPPGGTERRPNGMYLPEMCRETFQAYRTTDVGRKLIITKNVFIE
                     GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWL
                     HQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIAR
                     WLSTLEISGGGTGGSGGTGGSGGTGGSMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDG
                     TPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAF
                     IEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDDWPEFANWKTL
                     SVIMGVFVCCWLPFFILNCIRPFCGSGWTQPFCIDSNTFDVFVWFGWANSSLNPIIYAF
                     NADFRKAFSTLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIP
                     HAVGSSEDLKKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT"
     CDS             893..895
                     /label=V1.31
     CDS             1046..1048
                     /label=V2.53
     CDS             1214..1216
                     /label=F34.51
     misc_feature    1421..1423
                     /label=S222(5.42)A
     misc_feature    1433..1435
                     /label=S226(5.46)P
     misc_feature    1466..1468
                     /label=T5.57C
     primer_bind     complement(1474..1491)
                     /label=D1-insert-N1-rev
     primer_bind     complement(1477..1491)
                     /label=D1-insert-5.69I-rev
     primer_bind     1490..1511
                     /label=N3-fwd
     primer_bind     1490..1506
                     /label=N2-fwd
     misc_feature    1493..1495
                     /label=5.66
     primer_bind     complement(1496..1525)
                     /label=Primer 1
     primer_bind     1496..1518
                     /label=N4-fwd
     misc_feature    1496..1498
                     /label=5.67
     gene            1499..1579
                     /label=Loop 3
                     /label=Loop 3
     misc_feature    1499..1501
                     /label=5.68
     primer_bind     complement(1501..1522)
                     /label=ADRA2A-insert-5.75R-rev
     primer_bind     1502..1523
                     /label=N5-fwd
     misc_feature    1502..1504
                     /label=5.69
     primer_bind     complement(1505..1525)
                     /label=ADRA2A-insert-R249-rev
     misc_feature    1505..1507
                     /label=5.70
     primer_bind     complement(1507..1528)
                     /label=ADRA2A-insert-G250-rev
     primer_bind     1508..1529
                     /label=N6-fwd
     misc_feature    1508..1510
                     /label=5.71
     misc_feature    1511..1513
                     /label=1N-R
     primer_bind     1514..1537
                     /label=N7-fwd
     misc_feature    1514..1516
                     /label=5.73
     misc_feature    1517..1519
                     /label=5.74
     primer_bind     1520..1542
                     /label=N8-fwd
     primer_bind     1520..1538
                     /label=OVERLAP-REV
     misc_feature    1520..1522
                     /label=5.75
     misc_feature    1523..1579
                     /label=ICL3
     primer_bind     complement(1523..1552)
                     /label=OVERLAP-REV
     primer_bind     1523..1547
                     /label=LOOP-FWD
     misc_feature    1523..1525
                     /label=R249
     primer_bind     1526..1548
                     /label=N9-fwd
     misc_feature    1526..1528
                     /label=G250
     misc_feature    1529..1531
                     /label=P251
     misc_feature    1532..1534
                     /label=D252
     misc_feature    1535..1537
                     /label=A253
     primer_bind     1538..1560
                     /label=N10-fwd
     misc_feature    1538..1540
                     /label=2N
     misc_feature    1547..1549
                     /label=P257
     misc_feature    1559..1561
                     /label=3N
     primer_bind     complement(1567..1579)
                     /label=ADR-cpHT-NNTrun5-fwd
     misc_feature    1568..1570
                     /label=R264
     primer_bind     complement(1569..1579)
                     /label=ADR-cpHT-NNTrun4-P-fwd
     misc_feature    1577..1579
                     /label=G267
     misc_feature    1580..1582
                     /label=L5.95M
     misc_feature    1583..1585
                     /label=T5.96Y
     CDS             1586..1588
                     /codon_start=1
                     /label=P270L or P5.97
                     /translation="L"
     primer_bind     1589..1613
                     /label=cpHalotag-fwd
     primer_bind     1589..1613
                     /label=cpHalotag-N-all(P)-fwd
     CDS             1589..1603
                     /codon_start=1
                     /label=linker
                     /translation="PEMCR"
     primer_bind     1600..1617
                     /label=cpHalotag-Ntrun2-F-fwd
     primer_bind     1600..1615
                     /label=cpHalotag-Ntrun1-E-fwd
     CDS             1604..2056
                     /codon_start=1
                     /label=C
                     /note="/region_type=Domain"
                     /translation="ETFQAYRTTDVGRKLIITKNVFIEGTLPMGVVRPLTEVEMDHYRE
                     PFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
                     EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISG"
     primer_bind     complement(1604..1633)
                     /label=ADR-overlap-rev
     primer_bind     1604..1627
                     /label=cpHalotag-Ntrun5-fwd
     primer_bind     1604..1626
                     /label=cpHalotag-no linker-fwd
     misc_feature    1619..1621
                     /label=F6Y
     misc_feature    1628..1630
                     /label=9
     misc_feature    1643..1645
                     /label=14
     CDS             1655..1657
                     /label=T18E
     CDS             1658..1660
                     /label=Q19K
     misc_feature    1661..1663
                     /label=20
     misc_feature    1682..1684
                     /label=27
     misc_feature    1685..1687
                     /label=28
     misc_feature    1688..1690
                     /label=29
     primer_bind     complement(1808..1831)
                     /label=cphalotag-conoly-rev
     primer_bind     complement(1860..1880)
                     /label=cpHalotag-seq-rev
     CDS             2057..2110
                     /codon_start=1
                     /label=Circular permutation linker
                     /label=Circular permutation linker
                     /translation="GGTGGSGGTGGSGGTGGS"
     CDS             2111..2545
                     /codon_start=1
                     /label=N
                     /note="/region_type=Domain"
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDDWPEFA"
     region          2282..2284
                     /label=mutation
     primer_bind     2503..2533
                     /label=overlap-fwd
     primer_bind     complement(2508..2533)
                     /label=cpHalotag-no linker-rev
     primer_bind     complement(2511..2533)
                     /label=cpHalotag-Ctrun5-rev
     primer_bind     complement(2516..2539)
                     /label=cpHalotag-Ctrun3-E-rev
     primer_bind     complement(2516..2536)
                     /label=cpHalotag-Ctrun4-P-rev
     primer_bind     complement(2520..2542)
                     /label=cpHalotag-Ctrun2-F-rev
     primer_bind     complement(2523..2545)
                     /label=cpHalotag-Ctrun1-A-rev
     primer_bind     complement(2526..2545)
                     /label=cpHalotag-C-all(P)-rev
     region          2534..2545
                     /label=linker
     misc_feature    2546..2548
                     /label=C5-N
     misc_feature    2549..2551
                     /label=G6.34W
     primer_bind     2551..2573
                     /label=D1-insert-6.34L-fwd
     primer_bind     2551..2569
                     /label=D1-insert-6.33V-fwd
     primer_bind     2551..2564
                     /label=D1-insert-C1-fwd
     primer_bind     2551..2563
                     /label=D1-insert-6.31T-fwd
     primer_bind     2552..2574
                     /label=D1-insert-6.35K-fwd
     misc_feature    2552..2554
                     /label=6.35K
     CDS             2555..2557
                     /label=T6.36
     CDS             2567..2569
                     /label=I6.40
     CDS             2600..2602
                     /label=6.51
     misc_feature    2621..2623
                     /label=L6.58R
     misc_feature    2642..2644
                     /label=E302W
     CDS             2687..2689
                     /label=7.39
     primer_bind     complement(3056..3111)
                     /label=pdisplay-NheI-D1-rev
     CDS             3080..3088
                     /codon_start=1
                     /label=L
                     /translation="*AS"
     misc_feature    3089..3673
                     /label=IRES
     primer_bind     complement(3114..3136)
                     /label=R-cpEGFP
                     /note="can be used for conoly PCR"
     CDS             3677..4444
                     /codon_start=1
                     /label=ORF
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKDGKKKKKKSKTKCVIM"
     gene            3680..4393
                     /label=EGFP
     primer_bind     complement(3853..3898)
                     /label=ADRA2A-insert-5.75R-rev
     primer_bind     4192..4201
                     /label=ck1_L01_F
     misc_feature    4394..4441
                     /label=CAAX
     unsure          4651..4668
                     /label=BGH
     rep_origin      complement(4998..5617)
                     /direction=LEFT
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
                     /label=pBR322_origin
     CDS             6158..6688
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAV
                     SPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVG
                     HARLEPGEQFGWREPLMLLIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPHCI
                     SHDGYFLGRSKVR"
     CDS             complement(6209..6997)
                     /codon_start=1
                     /label=ORF frame 3
                     /label=ORF frame 3
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDQEHQGL
                     APAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALA
                     TRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(7107..7184)
                     /direction=LEFT
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
                     /label=SV40_origin
     gene            complement(7452..8312)
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
                     /label=Ampicillin
     promoter        complement(8354..8382)
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
                     /label=AmpR_promoter
     rep_origin      8461..8767
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
                     /label=f1_origin
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaccg gtaggactct
      841 gaacacctct gccatggacg ggactgggct ggtggtggag agggacttct ctgttcgtat
      901 cctcactgcc tgtttcctgt cgctgctcat cctgtccacg ctcctgggga acacgctggt
      961 ctgtgctgcc gttatcaggt tccgacacct gcggtccaag gtgaccaact tctttgtcat
     1021 ctccttggct gtgtcagatc tcttggtggc cgtcctggtc atgccctgga aggcagtggc
     1081 tgagattgct ggcttctggc cctttgggtc cttctgtaac atctgggtgg cctttgacat
     1141 catgtgctcc actgcatcca tcctcaacct ctgtgtgatc agcgtggaca ggtattgggc
     1201 tatctccagc cctttccggt atgagagaaa gatgaccccc aaggcagcct tcatcctgat
     1261 cagtgtggca tggaccttgt ctgtactcat ctccttcatc ccagtgcagc tcagctggca
     1321 caaggcaaaa cccacaagcc cctctgatgg aaatgccact tccctggctg agaccataga
     1381 caactgtgac tccagcctca gcaggacata tgccatctca gcctctgtaa taccctttta
     1441 catccctgtg gccatcatga ttgtctgcta caccaggatc tacaggattg ccaagcgtcg
     1501 cacccgcgtg ccacccagcc gccggggtcc ggacgccgtc gccgcgccgc cggggggcac
     1561 cgagcgcagg cccaacggta tgtacttgcc agagatgtgc cgtgagacct tccaggccta
     1621 ccgcaccacc gacgtcggcc gcaagctgat catcaccaag aacgttttta tcgagggtac
     1681 gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt accgcgagcc
     1741 gttcctgaat cctgttgacc gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc
     1801 cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac atggactggc tgcaccagtc
     1861 ccctgtcccg aagctgctgt tctggggcac cccaggcgtt ctgatcccac cggccgaagc
     1921 cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg gacatcggcc cgggtctgaa
     1981 tctgctgcaa gaagacaacc cggacctgat cggcagcgag atcgcgcgct ggctgtcgac
     2041 gctcgagatt tccggcggag gaacaggtgg ttctggtgga acagggggta gcggaggtac
     2101 aggaggaagt atggcggaga tcggaactgg attcccgttt gatccgcatt atgtggaagt
     2161 tctgggagag cgcatgcatt atgtggacgt tggtcctcgt gatgggacac cagtgctgtt
     2221 ccttcacggc aatccgacat cgtcgtacgt gtggcgtaat atcatcccgc acgttgcccc
     2281 cacgcaccgc tgcattgccc ctgacttaat tggtatgggg aaaagtgata agcctgatct
     2341 ggggtacttc tttgacgacc acgtacgctt catggatgct tttattgaag cattgggttt
     2401 ggaggaagta gttttggtga tccatgattg gggtagtgct ctggggttcc attgggccaa
     2461 gcgtaaccca gaacgcgtga aaggaattgc ctttatggag ttcatccgtc cgattccaac
     2521 atgggacgac tggcctgagt ttgcaaactg gaagactctg tcggtgatca tgggtgtgtt
     2581 tgtgtgctgt tggctacctt tcttcatctt gaactgcatt cggcccttct gtgggtctgg
     2641 gtggacgcag cccttctgca ttgattccaa cacctttgac gtgtttgtgt ggtttgggtg
     2701 ggctaattca tccttgaacc ccatcattta tgcctttaat gctgattttc ggaaggcatt
     2761 ttcaaccctc ttaggatgct acagactttg ccctgcgacg aataatgcca tagagacggt
     2821 gagtatcaat aacaatgggg ccgcgatgtt ttccagccat catgagccac gaggctccat
     2881 ctccaaggag tgcaatctgg tttacctgat cccacatgct gtgggctcct ctgaggacct
     2941 gaaaaaggag gaggcagctg gcatcgccag acccttggag aagctgtccc cagccctatc
     3001 ggtcatattg gactatgaca ctgacgtctc tctggagaag atccaaccca tcacacaaaa
     3061 cggtcagcac ccaacctgat aagctagcgc ccctctccct cccccccccc taacgttact
     3121 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3181 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3241 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     3301 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     3361 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     3421 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     3481 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     3541 tgtatgggat ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta
     3601 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     3661 taatatggcc acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3721 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3781 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3841 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     3901 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3961 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4021 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4081 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4141 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4201 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4261 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     4321 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     4381 cgagctgtac aaggatggta aaaagaagaa aaagaagtca aagacaaagt gtgtaattat
     4441 gtaagtcgac gaacaaaaac tcatctcaga agaggatctg aatgctgtgg gccaggacac
     4501 gcaggaggtc atcgtggtgc cacactcctt gccctttaag gtggtggtga tctcagccat
     4561 cctggccctg gtggtgctca ccatcatctc ccttatcatc ctcatcatgc ttggcagaag
     4621 aagccacgtt aggcggccgc tcgagatcag cctcgactgt gccttctagt tgccagccat
     4681 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4741 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4801 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4861 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagtggcggt aatacggtta
     4921 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4981 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5041 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5101 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5161 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5221 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5281 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5341 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5401 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     5461 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5521 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct
     5761 gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa
     5821 cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc
     5881 ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac
     5941 ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt
     6001 attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg
     6061 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     6121 ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg
     6181 tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     6241 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     6301 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     6361 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     6421 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct
     6481 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttgatca tcctgatcga
     6541 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     6601 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     6661 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     6721 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     6781 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     6841 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     6901 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     6961 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     7021 cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     7081 agctcagagg ccgaggaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     7141 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     7201 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     7261 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
     7321 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctggttc
     7381 tttccgcctc aggactcttc ctttttcaat aaatcaatct aaagtatata tgagtaaact
     7441 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7501 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7561 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7621 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7681 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7741 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7801 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7861 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7921 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7981 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8041 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8101 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8161 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8221 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8281 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8341 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8401 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     8461 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8521 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8581 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8641 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8701 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8761 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     8821 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8881 atattaacgc ttacaattta c
//

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