LOCUS       pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE      6848 bp    DNA     linear   UNA 
FEATURES             Location/Qualifiers
     misc_feature    46..212
                     /note="ITR"
     promoter        237..1171
                     /note="CAG promoter"
     misc_feature    1180..1213
                     /note="loxP2272"
     misc_feature    1276..1309
                     /note="loxP"
     CDS             complement(1320..3140)
                     /label="iGluSnFR4f"
     misc_feature    complement(3157..3190)
                     /note="loxP2272"
     misc_feature    complement(3261..3294)
                     /note="loxP"
     misc_feature    3336..3924
                     /note="WPRE"
     misc_feature    3952..4184
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(4212..4378)
                     /label="ITR"
     Origin          complement(4616..5204)
                     /label="ori"
     gene            complement(5378..6235)
                     /note="Amp"
     Origin          complement(6366..6821)
                     /label="f1-ori"
ORIGIN
        1 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGCGC GCTCGCTCGC
       61 TCACTGAGGC CGCCCGGGCA AAGCCCGGGC GTCGGGCGAC CTTTGGTCGC CCGGCCTCAG
      121 TGAGCGAGCG AGCGCGCAGA GAGGGAGTGG CCAACTCCAT CACTAGGGGT TCCTTGTAGT
      181 TAATGATTAA CCCGCCATGC TACTTATCTA CGTAGCCATG CTCTAGGAAG AGTACCATTG
      241 ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA
      301 TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA
      361 AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC
      421 ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC
      481 ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC
      541 CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC GGGGGGGGGG
      601 GGGGGGCGCG CGCCAGGCGG GGCGGGGCGG GGCGAGGGGC GGGGCGGGGC GAGGCGGAGA
      661 GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT TTCCTTTTAT GGCGAGGCGG
      721 CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG CGGGAGTCGC TGCGACGCTG
      781 CCTTCGCCCC GTGCCCCGCT CCGCCGCCGC CTCGCGCCGC CCGCCCCGGC TCTGACTGAC
      841 CGCGTTACTC CCACAGGTGA GCGGGCGGGA CGGCCCTTCT CCTCCGGGCT GTAATTAGCG
      901 CTTGGTTTAA TGACGGCTTG TTTCTTTTCT GTGGCTGCGT GAAAGCCTTG AGGGGCTCCG
      961 GGAGGGCCCT TTGTGCGGGG GGAGCGGCTC GGGGCTGTCC GCGGGGGGAC GGCTGCCTTC
     1021 GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC TCTAGAGCCT
     1081 CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC GTGCTGGTTA
     1141 TTGTGCTGTC TCATCATTTT GGCAAAGAAT TGGATCCCCA TAACTTCGTA TAAAGTATCC
     1201 TATACGAAGT TATATCAAAA TAGGAAGACC AATGCTTCAC CATCGACCCG AATTGCCAAG
     1261 CATCACCATC GACCCATAAC TTCGTATAAT GTATGCTATA CGAAGTTATA CTAGAGCTTT
     1321 CAACGTGGCT TCTTCTGCCA AAGCATGATG AGGATGATAA GGGAGATGAT GGTGAGCACC
     1381 ACCAGGGCCA GGATGGCTGA GATCACCACC ACCTTAAAGG GCAAGGAGTG TGGCACCACG
     1441 ATGACCTCCT GCGTGTCCTG GCCCACAGCA TTCAGATCCT CTTCTGAGAT GAGTTTTTGT
     1501 TCGTCGACCT GCAGTTTCAG TGCCTTGTCA TTCGGTTCTT TGAACAGTGC TTTCATTTCG
     1561 TCTGACAGTT CGAAATTCAT GACCAGGGGG TTGTTAAAGT TGTACTCCAG CTTGTGCCCC
     1621 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTTAGCT CGATGCGGTT CACCAGGGTG
     1681 TCGCCCTCTA ACTTCACCTC GGCGCGGGTC TTGTAGGTTC CGTCGTCCTT GAAGCTGATG
     1741 GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG CTGCTTCATG
     1801 TGGTCGGGGT AGCGGGCGAA GCACTGCACG CCGTAGCTCA GGGTGGTCAC GAGGGTGGGC
     1861 CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATGAACTTCA GGGTTAGCTT GCCGTTGGTG
     1921 GCATCGCCCT CGCCCTCGCC GCGCACGCTG AACTTGTGGC CGTTTACGTC GCCGTCCAGC
     1981 TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAT GCTCCCTCCG
     2041 GTACCGCCCT TGTACAGCTC GTCCATGCCG AGAGAGTTCC CGGTGGCGGT CACGAACTCC
     2101 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGCACGGA CTGATAGCTC
     2161 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG
     2221 TGGTCGGCGA GCTGCACGCT GCCGTCCTCC ACGTTGTGGT GGATGAAGAA GTTCGCCTTG
     2281 ATGCCGTTCT TCTGCTTGTC GGCGGTGATA TAGACCGTGT GGTTCACCAG AATTGGATTT
     2341 TTGAACCACT TATCAAACCA TTTTTCCGCT TCACCGGAGG TCTGCACCTG AGCGATGGTG
     2401 TCATCCATCA GCTTTTTGAA CTGCGGATCA TCTTTACGCA ACATACAACC GTAGGCCTCC
     2461 TGAGACTGCG GCTTGCCGAC GATTTCCCAG TCGTCTGGTT TCTTCGCTTT CGCACGTTCA
     2521 CCGGCCAGCA GTACGTCATC CATCATAAAG GCAACGGCAC GACCGCTTTC CAGGGTGCGG
     2581 AAAGAGTCAC CGTGATCTTT GGCGCTGATG ATGCGCATAT TCATTTTTTG CTCTTCATTC
     2641 AGTTTGTTGA GCAAAACTTC AGAGGTAGTG CCGGAAGTGA CGACTACGGC TTTGTCTTTC
     2701 AGGTTGGCAA AATCTTTGAT ATCGCCACCC TTTTTGGTCA ACAGGCGCGT ACCGACCACG
     2761 AAAATAGTGT CAGAGAAAGC CGCGAATTTT TGGCGTTCGA CGTTGTTGGT GGTAGAACCA
     2821 CATTCAAAAT CGTAAGTGCC GTTTTGCAGT AGTGGAATAC GGTTTTGTGA AGTAATCGGA
     2881 ATCAGTTTTA CCTGCAAGTC CGGTTTGTTG AGTTTCTTTT TCACTGCTTC AACAATGGCG
     2941 TTGGAGTATT CCTGCGAGTA ACCCACCACT TTGTGCTGAC TGTCTTGATA AGAGAAAGGC
     3001 ACTGAAGAGT CACGGTGACC GACGACAATC ACACCGTTTT TGGCGATTTT GTCCAGCGTG
     3061 CTGCCTGCGG CGGATCCGTC ACCAGTGGAA CCTGGAACCC AGAGCAGCAG TACCCATAGC
     3121 AGGAGTGTGT CTGTCTCCAT GGTGGCGGAT CCTAGTATAA CTTCGTATAG GATACTTTAT
     3181 ACGAAGTTAT CATTGGGATT CTTCCTATTT TGATCCAAGC ATCACCATCG ACCCTCTAGT
     3241 CCAGATCTCA CCATCGACCC ATAACTTCGT ATAGCATACA TTATACGAAG TTATGTCCCT
     3301 CGAAGAGGTT CGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT
     3361 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG
     3421 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT
     3481 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG
     3541 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC
     3601 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG
     3661 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT
     3721 TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC
     3781 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG
     3841 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA
     3901 TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGACC CGGGCGGCCG CTTCGAGCAG
     3961 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT
     4021 GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA
     4081 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGATGTGGG
     4141 AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGATAAG GATCTTCCTA
     4201 GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA TTAACTACAA GGAACCCCTA
     4261 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA
     4321 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC
     4381 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
     4441 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
     4501 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
     4561 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     4621 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     4681 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     4741 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     4801 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     4861 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     4921 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     4981 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     5041 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     5101 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     5161 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
     5221 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     5281 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
     5341 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
     5401 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
     5461 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
     5521 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
     5581 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
     5641 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
     5701 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
     5761 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
     5821 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
     5881 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
     5941 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
     6001 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
     6061 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
     6121 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
     6181 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
     6241 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     6301 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
     6361 CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG
     6421 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC
     6481 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA
     6541 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC
     6601 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG
     6661 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA
     6721 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC
     6781 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT
     6841 GCGCAACT
//
