LOCUS       pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE      6528 bp    DNA     linear   UNA 
FEATURES             Location/Qualifiers
     misc_feature    46..211
                     /label="ITR"
     promoter        256..703
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
     intron          complement(725..857)
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     protein_bind    879..926
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the
                     Saccharomycescerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1034..1156)
                     /label="NGR"
     CDS             complement(1157..2824)
                     /label="iGluSnFR4f"
     protein_bind    complement(2866..2913)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the
                     Saccharomycescerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    complement(3010..3598)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(3647..3768)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3893..4058)
                     /label="ITR"
     Origin          complement(4296..4884)
                     /label="ori"
     misc_feature    complement(5058..5915)
                     /label="Amp"
     Origin          complement(6046..6501)
                     /label="f1_ori"
ORIGIN
        1 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGCGC GCTCGCTCGC
       61 TCACTGAGGC CGCCCGGGCA AAGCCCGGGC GTCGGGCGAC CTTTGGTCGC CCGGCCTCAG
      121 TGAGCGAGCG AGCGCGCAGA GAGGGAGTGG CCAACTCCAT CACTAGGGGT TCCTTGTAGT
      181 TAATGATTAA CCCGCCATGC TACTTATCTA CGTAGCCATG CTCTAGGAAG ATCTCTGCAG
      241 AGGGCCCTGC GTATGAGTGC AAGTGGGTTT TAGGACCAGG ATGAGGCGGG GTGGGGGTGC
      301 CTACCTGACG ACCGACCCCG ACCCACTGGA CAAGCACCCA ACCCCCATTC CCCAAATTGC
      361 GCATCCCCTA TCAGAGAGGG GGAGGGGAAA CAGGATGCGG CGAGGCGCGT GCGCACTGCC
      421 AGCTTCAGCA CCGCGGACAG TGCCTTCGCC CCCGCCTGGC GGCGCGCGCC ACCGCCGCCT
      481 CAGCACTGAA GGCGCGCTGA CGTCACTCGC CGGTCCCCCG CAAACTCCCC TTCCCGGCCA
      541 CCTTGGTCGC GTCCGCGCCG CCGCCGGCCC AGCCGGACCG CACCACGCGA GGCGCGAGAT
      601 AGGGGGGCAC GGGCGCGACC ATCTGCGCTG CGGCGCCGGC GACTCAGCGC TGCCTCAGTC
      661 TGCGGTGGGC AGCGGAGGAG TCGTGTCGTG CCTGAGAGCG CAGTCGAATT CAAGCTGCTA
      721 GCAGGTAAGT ATCAAGGTTA CAAGACAGGT TTAAGGAGAC CAATAGAAAC TGGGCTTGTC
      781 GAGACAGAGA AGACTCTTGC GTTTCTGATA GGCACCTATT GGTCTTACTG ACATCCACTT
      841 TGCCTTTCTC TCCACAGGTG ATAAGGATCC GGCGCGCCGA AGTTCCTATT CCGAAGTTCC
      901 TATTCTCTAG AAAGTATAGG AACTTCCCTT TGGCCGGAGC GGCCAAAGCG CTCTGAAGTT
      961 CCTATTCCGA AGTTCCTATT CTGAACTAAG TATAGGAACT TCGTTTAAAC TTAATTAAAC
     1021 GCGTACCGGT TTATCAACAG GGGCCCAACA CCGTCCACAG AACCAATGCC AGTCCCAGTG
     1081 GAGCCAGACT ACATGCAAGG GCAGGAAGGG CACCTGATCC TTCTGCATCT CCTGTTCCAG
     1141 ATGCACCACT ACCTGCCAGA TCCTCTTCTG AGATGAGTTT TTGTTCGTCG ACCTGCAGTT
     1201 TCAGTGCCTT GTCATTCGGT TCTTTGAACA GTGCTTTCAT TTCGTCTGAC AGTTCGAAAT
     1261 TCATGACCAG GGGGTTGTTA AAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT
     1321 CCTTGAAGTC GATGCCCTTT AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCTAACTTCA
     1381 CCTCGGCGCG GGTCTTGTAG GTTCCGTCGT CCTTGAAGCT GATGGTGCGC TCCTGGACGT
     1441 AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGG
     1501 CGAAGCACTG CACGCCGTAG CTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT
     1561 TGCCGGTGGT GCAGATGAAC TTCAGGGTTA GCTTGCCGTT GGTGGCATCG CCCTCGCCCT
     1621 CGCCGCGCAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA
     1681 CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCATGCTCCC TCCGGTACCG CCCTTGTACA
     1741 GCTCGTCCAT GCCGAGAGAG TTCCCGGTGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT
     1801 CGCGCTTCTC GTTGGGGTCT TTGCTCAGCA CGGACTGATA GCTCAGGTAG TGGTTGTCGG
     1861 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA
     1921 CGCTGCCGTC CTCCACGTTG TGGTGGATGA AGAAGTTCGC CTTGATGCCG TTCTTCTGCT
     1981 TGTCGGCGGT GATATAGACC GTGTGGTTCA CCAGAATTGG ATTTTTGAAC CACTTATCAA
     2041 ACCATTTTTC CGCTTCACCG GAGGTCTGCA CCTGAGCGAT GGTGTCATCC ATCAGCTTTT
     2101 TGAACTGCGG ATCATCTTTA CGCAACATAC AACCGTAGGC CTCCTGAGAC TGCGGCTTGC
     2161 CGACGATTTC CCAGTCGTCT GGTTTCTTCG CTTTCGCACG TTCACCGGCC AGCAGTACGT
     2221 CATCCATCAT AAAGGCAACG GCACGACCGC TTTCCAGGGT GCGGAAAGAG TCACCGTGAT
     2281 CTTTGGCGCT GATGATGCGC ATATTCATTT TTTGCTCTTC ATTCAGTTTG TTGAGCAAAA
     2341 CTTCAGAGGT AGTGCCGGAA GTGACGACTA CGGCTTTGTC TTTCAGGTTG GCAAAATCTT
     2401 TGATATCGCC ACCCTTTTTG GTCAACAGGC GCGTACCGAC CACGAAAATA GTGTCAGAGA
     2461 AAGCCGCGAA TTTTTGGCGT TCGACGTTGT TGGTGGTAGA ACCACATTCA AAATCGTAAG
     2521 TGCCGTTTTG CAGTAGTGGA ATACGGTTTT GTGAAGTAAT CGGAATCAGT TTTACCTGCA
     2581 AGTCCGGTTT GTTGAGTTTC TTTTTCACTG CTTCAACAAT GGCGTTGGAG TATTCCTGCG
     2641 AGTAACCCAC CACTTTGTGC TGACTGTCTT GATAAGAGAA AGGCACTGAA GAGTCACGGT
     2701 GACCGACGAC AATCACACCG TTTTTGGCGA TTTTGTCCAG CGTGCTGCCT GCGGCGGATC
     2761 CGTCACCAGT GGAACCTGGA ACCCAGAGCA GCAGTACCCA TAGCAGGAGT GTGTCTGTCT
     2821 CCATGGTGGC GAATTCAGAT CTACTAGTGG CCGGCCAGTT TAAACGAAGT TCCTATACTT
     2881 TCTAGAGAAT AGGAACTTCG GAATAGGAAC TTCCATTGCG ATCGCTCCTA TTTTGATCCA
     2941 AGAAGTTCCT ATACTTAGTT CAGAATAGGA ACTTCGGAAT AGGAACTTCC CTGCAGGAAG
     3001 CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC
     3061 TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT
     3121 GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT
     3181 GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA
     3241 ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC
     3301 CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG
     3361 GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT
     3421 TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT
     3481 TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT
     3541 CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT
     3601 CGATACCGTC GACCTCGACC CGGGCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT
     3661 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT
     3721 GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT
     3781 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGATGTGGG AGGTTTTTTA AAGCAAGTAA
     3841 AACCTCTACA AATGTGGTAA AATCGATAAG GATCTTCCTA GAGCATGGCT ACGTAGATAA
     3901 GTAGCATGGC GGGTTAATCA TTAACTACAA GGAACCCCTA GTGATGGAGT TGGCCACTCC
     3961 CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG
     4021 CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC TGCATTAATG AATCGGCCAA
     4081 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
     4141 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
     4201 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
     4261 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     4321 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     4381 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     4441 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     4501 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     4561 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     4621 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     4681 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
     4741 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     4801 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     4861 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     4921 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     4981 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     5041 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     5101 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     5161 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT
     5221 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     5281 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     5341 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     5401 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     5461 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     5521 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     5581 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     5641 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     5701 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     5761 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     5821 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     5881 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     5941 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     6001 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTAAATT GTAAGCGTTA
     6061 ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG
     6121 CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG
     6181 TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA
     6241 AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG
     6301 GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT
     6361 GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG
     6421 CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA
     6481 ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT GCGCAACT
//
