LOCUS       pCH196_His6-smt3        6445 bp DNA     circular SYN 12-FEB-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pCH196
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 12, 2025 from SnapGene Viewer 6.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..44
                     /label=lac\operator
                     /note="vntifkey=""""4"""" - label=lac\operator -  
                     complement(343..367)"
                     /note="vntifkey=""4"" - label=lac\operator -  
                     complement(343..367)"
                     /note="color: #cc0000; direction: RIGHT"
     CDS             86..1285
                     /codon_start=1
                     /label=ORF
                     /note="color: #cc0000"
                     /translation="MGSSHHHHHHSSGLVPRGSHMASMSDSEVNQEAKPEVKPEVKPET
                     HINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDL
                     DMEDNDIIEAHREQIGGSMIEFIEFEIEDLGVMEIDVYDIEVDGNHNFFGNDILVHNSV
                     YLNGTSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPV
                     PWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKF
                     EGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHG
                     "
     misc_feature    98..115
                     /label=His6
                     /note="color: #cc0000; direction: RIGHT"
     CDS             125..142
                     /gene="Thrombin site"
                     /label=Thrombin site
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    158..448
                     /label=Smt3
                     /note="note=""direct strand translation""  
                     translation=""SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                      
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGSEFELRR
                      QACGRTRAPPPPPLRSGC*"""
                     /note="color: #cc0000; direction: RIGHT"
     CDS             452..568
                     /codon_start=1
                     /label=AesC
                     /note="color: #cc0000"
                     /translation="MIEFIEFEIEDLGVMEIDVYDIEVDGNHNFFGNDILVHN"
     misc_feature    569..583
                     /label=C-Extein
                     /note="color: #cc0000; direction: RIGHT"
     CDS             590..1282
                     /codon_start=1
                     /label=sfGFP
                     /note="color: #cc0000"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHG"
     CDS             1307..1324
                     /codon_start=1
                     /label=His6
                     /note="color: #cc0000"
                     /translation="HHHHHH"
     CDS             complement(2025..2837)
                     /label=KanR
                     /note="color: #cc0000; direction: LEFT"
     CDS             complement(4977..6068)
                     /codon_start=1
                     /label=lacI
                     /note="color: #cc0000"
                     /translation="VVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAM
                     AELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVER
                     SGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGD
                     WSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTED
                     SSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTA
                     SPRALADSLMQLARQVSRLESGQ"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gataattttg
       61 tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag
      121 cggcctggtg ccgcgcggca gccatatggc tagcatgtcg gactcagaag tcaatcaaga
      181 agctaagcca gaggtcaagc cagaagtcaa gcctgagact cacatcaatt taaaggtgtc
      241 cgatggatct tcagagatct tcttcaagat caaaaagacc actcctttaa gaaggctgat
      301 ggaagcgttc gctaaaagac agggtaagga aatggactcc ttaagattct tgtacgacgg
      361 tattagaatt caagctgatc agacccctga agatttggac atggaggata acgatattat
      421 tgaggctcac agagaacaga ttggtggatc catgatcgag ttcatcgagt tcgaaattga
      481 agatctgggc gtgatggaaa tcgatgtgta tgatattgaa gtggatggca accacaactt
      541 ttttggcaat gatattctgg tgcacaacag cgtgtatctg aatggtacca gcaaaggcga
      601 agaactgttt accggcgtgg tgccgattct ggttgaactg gatggcgatg ttaatggcca
      661 taagtttagc gtgcgcggcg aaggcgaagg cgatgcgacc aacggcaaac tgaccctgaa
      721 gtttatttgc accaccggca aactgccggt gccgtggccg accctggtga ccaccctgac
      781 ctatggcgtg cagtgcttta gccgctatcc ggatcacatg aaacgccatg atttctttaa
      841 gtccgcgatg ccggaaggct atgttcagga acgcaccatt agctttaaag atgatggcac
      901 ctataagacc cgcgcggaag tgaaatttga aggcgatacc ctggtgaacc gcattgaact
      961 gaaaggcatt gacttcaaag aagatggcaa cattctgggc cataaactgg agtacaattt
     1021 caacagccat aacgtgtata ttaccgcgga caaacagaag aacggcatca aagcgaattt
     1081 caaaatccgc cataatgtgg aagatggcag cgtgcagctg gcggatcact atcagcagaa
     1141 taccccgatc ggcgatggcc cggtgctgct gccggacaat cactacctga gcacccagtc
     1201 cgtgctgagc aaagatccga atgagaaacg cgatcacatg gttctgctgg agtttgtgac
     1261 cgcggcgggt atcactcatg gttaaaagct tgcggccgca ctcgagcacc accaccacca
     1321 ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     1381 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     1441 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     1501 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     1561 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     1621 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     1681 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     1741 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     1801 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     1861 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     1921 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1981 tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc
     2041 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2101 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2161 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     2221 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2281 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2341 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     2401 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     2461 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     2521 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     2581 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     2641 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2701 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     2761 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     2821 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     2881 gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2941 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3001 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3061 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     3121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3721 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3781 catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     3841 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     3901 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3961 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     4021 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     4081 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     4141 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     4201 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     4261 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     4321 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     4381 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     4441 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     4501 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     4561 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     4621 tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     4681 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     4741 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     4801 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     4861 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     4921 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     4981 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5041 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     5101 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     5161 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     5221 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     5281 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     5341 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     5401 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     5461 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     5521 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     5581 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     5641 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     5701 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     5761 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     5821 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     5881 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     5941 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     6001 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     6061 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     6121 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     6181 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     6241 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     6301 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     6361 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     6421 gatcgagatc tcgatcccgc gaaat
//
