LOCUS       pJPC062                10095 bp ds-DNA     circular     21-JAN-2024
DEFINITION  .
KEYWORDS    "Creator" "Resistance" "Origin(s) of Rep" "Module" "Empty Module
            Cassette" "Plant Selection Promoter" "Plant Selection" "Nuclease
            Promoter" "Nuclease" "Guide Promoter" "Guide Terminator" "Gene"
            "Viral Replicon" "Parent Plasmids" "Description" "Box"
FEATURES             Location/Qualifiers
     misc_feature    1..26
                     /label="T-DNA Border (L)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     promoter        128..896
                     /label="2x 35S"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    897..1260
                     /label="TRV2 5' Sequence"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1261..1424
                     /label="TRV2 Viral Promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1425..1456
                     /label="TRV2 5' Sequence"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1457..2068
                     /label="Coat Protein"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2069..2237
                     /label="TRV2 Sequence"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2238..2424
                     /label="PeBV sgPromoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          2371..2390
                     /label="oJPC077"
                     /note="sequence: acaactcggtttgctgacct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2434..3024
                     /label="CinH"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_feature    3001..3021
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3035..3429
                     /label="TRV 3' sequence"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3122..3144)
                     /label="oJPC078"
                     /note="sequence: accgatcaatcaagatcagtcga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3532..3794
                     /label="Nos Terminator"
                     /ApEinfo_revcolor="#a40000"
                     /ApEinfo_fwdcolor="#a40000"
     misc_feature    3835..3860
                     /label="T-DNA Border (R)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    complement(4903..5902)
                     /label="STA region from pVS1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      complement(6496..7496)
                     /label="pVS1 ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(7906..8166)
                     /label="bom site from pBR322"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    8877..9671
                     /label="KanR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg
      121 gcgcgccaag cttgcatgcc tgcaggtcaa catggtggag cacgacactc tcgtctactc
      181 caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag
      241 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
      301 gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat
      361 cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat
      421 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatggtca
      481 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
      541 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
      601 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
      661 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
      721 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
      781 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
      841 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggataa
      901 aacattgcac ctatggtgtt gccctggctg gggtatgtca gtgatcgcag tagaatgtac
      961 taattgacaa gttggagaat acggtagaac gtccttatcc aacacagcct ttatccctct
     1021 ccctgacgag gtttttgtca gtgtaatatt tctttttgaa ctatccagct tagtaccgta
     1081 cgggaaagtg actggtgtgc ttatctttga aatgttactt tgggtttcgg ttctttaggt
     1141 tagtaagaaa gcacttgtct tctcatacaa aggaaaacct gagacgtatc gcttacgaaa
     1201 gtagcaatga aagaaaggtg gtggttttaa tcgctaccgc aaaaacgatg gggtcgtttt
     1261 aattaacttc tcctacgcaa gcgtctaaac ggacgttggg gttttgctag tttctttaga
     1321 gaaaactagc taagtcttta atgttatcat tagagatggc ataaatataa tacttgtgtc
     1381 tgctgataag atcattttaa tttggacgat tagacttgtt gaactacagg ttactgaatc
     1441 acttgcgcta atcaacatgg gagatatgta cgatgaatca tttgacaagt cgggcggtcc
     1501 tgctgacttg atggacgatt cttgggtgga atcagtttcg tggaaagatc tgttgaagaa
     1561 gttacacagc ataaaatttg cactacagtc tggtagagat gagatcactg ggttactagc
     1621 ggcactgaat agacagtgtc cttattcacc atatgagcag tttccagata agaaggtgta
     1681 tttcctttta gactcacggg ctaacagtgc tcttggtgtg attcagaacg cttcagcgtt
     1741 caagagacga gctgatgaga agaatgcagt ggcgggtgtt acaaatattc ctgcgaatcc
     1801 aaacacaacg gttacgacga accaagggag tactactact accaaggcga acactggctc
     1861 gactttggaa gaagacttgt acacttatta caaattcgat gatgcctcta cagctttcca
     1921 caaatctcta acttcgttag agaacatgga gttgaagagt tattaccgaa ggaactttga
     1981 gaaagtattc gggattaagt ttggtggagc agctgctagt tcatctgcac cgcctccagc
     2041 gagtggaggt ccgatacgtc ctaatcccta gggatttaag gacgtgaact ctgttgagat
     2101 ctctgtgaaa ttcagagggt gggtgatacc atattcactg atgccattag cgacatctaa
     2161 atagggctaa ttgtgactaa tttgagggaa tttcctttac cattgacgtc agtgtcgttg
     2221 gtagcatttg agtttcggag catcttgttc tggggtttca cactatcttt agagaaagtg
     2281 ttaagttaat taagttatct taattaagag cataattata ctgatttgtc tctcgttgat
     2341 agagtctatc attctgttac taaaaatttg acaactcggt ttgctgacct actggttact
     2401 gtatcactta cccgagttaa cgagtctagA AACATGGGGA AGGGACAAAA GGTTGGCTAC
     2461 GTTCGTGTTT CATCGGTTGA GCAAAACACT GGGCGGCAGC TCGAGGGCAT CGAGGTGGAT
     2521 AGGATCTTCG TGGACAGGGC TTCCGGCAAG AATACCGATA GACCGAAGTT CCAGGAAATG
     2581 CTCAACTACG TCAGGGAAGG CGACCGCGTG ATCGTCCACT CAATGGATAG GTTCGCTAGG
     2641 TCCCTCAAGG ACTTGGTTAC CGAGGTCGAC AAATTGGTCA AGAGGGGGAT TGCCATTCAA
     2701 TTCGTCAAGG AGAACATCAC TTTCACCGCA CAGTCCACTC CAATGGACAA CCTCATGCTG
     2761 CAGCTTATGG GCGCCTTCGC GCAATTTGAG AGAGAGATTA TACTTGAGCG GCAGAAGGAG
     2821 GGTATTAAGA TCGCTGCCGC CCAAGGAAAG TACAAAGGTA GGGTGCATAA ACTCAACCCG
     2881 GACCAGGCTA AGGCTTTGCT ACAAGCGTGG AAGGAGGGCA AGTATTCTAG CAAGGTTGAC
     2941 CTCGCCAAAG CGTTCGGCAT ATCCAGGCAA GCCGTCTACA GATACCTCAA GCAGATCAAT
     3001 CCTAAGAAAA AGCGGAAAGT GTGAAGTGcc gggcatgtcc cgaagacatt aaactacggt
     3061 tctttaagta gatccgtgtc tgaagtttta ggttcaattt aaacctacga gattgacatt
     3121 ctcgactgat cttgattgat cggtaagtct tttgtaattt aattttcttt ttgattttat
     3181 tttaaattgt tatctgtttc tgtgtataga ctgtttgaga tcggcgtttg gccgactcat
     3241 tgtcttacca taggggaacg gactttgttt gtgttgttat tttatttgta ttttattaaa
     3301 attctcaacg atctgaaaaa gcctcgcggc taagagattg ttggggggtg agtaagtact
     3361 tttaaagtga tgatggttac aaaggcaaaa ggggtaaaac ccctcgccta cgtaagcgtt
     3421 attacgcccg tctgtactta tatcagtaca ctgacgagtc cctaaaggac gaaacgggag
     3481 aacgctagcc accaccacca ccaccacgtg tgaattacag gtgaccagct cgaatttccc
     3541 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     3601 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     3661 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     3721 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     3781 tatgttacta gatcgggaat taaactatca gtgtttgaca ggatatattg gcgggtaaac
     3841 ctaagagaaa agagcgttta ttagaataaT cggatattta aaagggcgtg aaaaggttta
     3901 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     3961 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     4021 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     4081 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     4141 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     4201 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     4261 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     4321 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     4381 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     4441 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     4501 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     4561 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     4621 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     4681 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     4741 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     4801 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     4861 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     4921 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     4981 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     5041 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     5101 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     5161 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     5221 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     5281 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     5341 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     5401 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     5461 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     5521 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     5581 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     5641 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     5701 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     5761 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     5821 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     5881 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     5941 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     6001 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     6061 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     6121 cggttggcca ggcgtaagcg gctgggttgC ctgccggccc tgcaatggca ctggaacccc
     6181 caagcccgag gaatcggcgt gaGcggtcgc aaaccatccg gcccggtaca aatcggcgcg
     6241 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     6301 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     6361 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     6421 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     6481 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     6541 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     6601 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     6661 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     6721 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     6781 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     6841 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     6901 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     6961 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     7021 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     7081 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     7141 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     7201 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     7261 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     7321 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     7381 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     7441 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     7501 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     7561 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     7621 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     7681 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     7741 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     7801 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     7861 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     7921 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     7981 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     8041 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8101 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8161 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8221 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8281 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8341 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8401 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8461 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8521 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8581 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8641 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8701 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8761 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8821 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa
     8881 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
     8941 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
     9001 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
     9061 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
     9121 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
     9181 ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
     9241 tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
     9301 gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca
     9361 tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
     9421 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
     9481 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
     9541 tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct
     9601 tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc
     9661 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
     9721 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
     9781 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
     9841 cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     9901 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     9961 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
    10021 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
    10081 ctgttggctg gctgg
//