LOCUS       Exported                6905 bp ds-DNA     circular SYN 13-JUN-2024
DEFINITION  mStayGold in pAAV-P3-Alb1-mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 13, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Resistance markers: Ampicillin
            This file was created using tools provided by Twist Bioscience
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="mStayGold in pAAV-P3-Alb1-mNeonGreen"
     CDS             1..675
                     /codon_start=1
                     /product="bright and highly photostable monomeric green 
                     fluorescent protein (Ando et al., 2023)"
                     /label=mStayGold(J)
                     /note="includes multiple mutations plus an N-terminal 
                     iinker sequence"
                     /translation="VSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGK
                     YVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSI
                     HAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTLQ
                     YPLLSDESKCVEAYQNTIIKPLHNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETLE
                     AHL"
     misc_feature    723..1311
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(1194..1205)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature_   1326..1553
                     /label=bGH_PA
     polyA_signal    1329..1553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1598..1738
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      1813..2268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature_   1830..2136
                     /label=f1
     misc_feature_   2550..2654
                     /label=AmpR_promoter
     promoter        2550..2654
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature_   2655..3515
                     /label=ampR
     CDS             2655..3515
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature_   3686..4274
                     /label=colE1_high_copy
     rep_origin      3686..4274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature_   4507..4640
                     /label=LAAV-2_ITR
     CDS             join(5025..6905,1..675)
                     /codon_start=1
                     /product="Ando et al. (2024) Not mStayGold(E138D)"
                     /label=Alb-mStayGold
                     /translation="MKWVTFLLLLFVSGSAFSRGVFRREAHKSEIAHRYNDLGEQHFKG
                     LVLIAFSQYLQKCSYDEHAKLVQEVTDFAKTCVADESAANCDKSLHTLFGDKLCAIPNL
                     RENYGELADCCTKQEPERNECFLQHKDDNPSLPPFERPEAEAMCTSFKENPTTFMGHYL
                     HEVARRHPYFYAPELLYYAEQYNEILTQCCAEADKESCLTPKLDGVKEKALVSSVRQRM
                     KCSSMQKFGERAFKAWAVARLSQTFPNADFAEITKLATDLTKVNKECCHGDLLECADDR
                     AELAKYMCENQATISSKLQTCCDKPLLKKAHCLSEVEHDTMPADLPAIAADFVEDQEVC
                     KNYAEAKDVFLGTFLYEYSRRHPDYSVSLLLRLAKKYEATLEKCCAEANPPACYGTVLA
                     EFQPLVEEPKNLVKTNCDLYEKLGEYGFQNAILVRYTQKAPQVSTPTLVEAARNLGRVG
                     TKCCTLPEDQRLPCVEDYLSAILNRVCLLHEKTPVSEHVTKCCSGSLVERRPCFSALTV
                     DETYVPKEFKAETFTFHSDICTLPEKEKQIKKQTALAELVKHKPKATAEQLKTVMDDFA
                     QFLDTCCKAADKDTCFSTEGPNLVTRCKDALAPGGGGGSGGGGSGGGGSVDVSTGEELF
                     TGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKLPMSWAALGTSFGYG
                     MKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSIHAKHQHFMKNGTYHNIVEFTGQ
                     DFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTLQYPLLSDESKCVEAYQNTIIKPL
                     HNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETLEAHL"
ORIGIN
        1 gtgtctacag gcgaggagct gtttaccggc gtggtgccct tcaagttcca gctgaagggc
       61 accatcaacg gcaagagctt caccgtggaa ggcgagggcg agggcaatag ccacgagggc
      121 agccacaaag gcaagtacgt gtgcaccagc ggcaaactgc caatgtcttg ggccgccctg
      181 ggaactagct tcggctatgg catgaagtac tacaccaagt accccagcgg cctgaagaac
      241 tggttccacg aggtgatgcc cgagggcttc acctacgaca gacacatcca gtacaagggc
      301 gacggcagca tccacgccaa gcaccagcac ttcatgaaga acggcaccta ccacaacatc
      361 gtggagttca ccggccagga cttcaaggag aacagccccg tgctgaccgg cgacatggac
      421 gtgagcctgc ccaacgaggt gcagcacatc cccatagatg acggcgtgga gtgcacagtg
      481 accctgcagt accctctgct gagcgacgag agcaagtgcg tggaggccta ccagaacacc
      541 atcatcaagc ccctgcacaa tcagccagcc cccgatgtgc cattccactg gatcagaaag
      601 cagtacaccc agagcaagga cgacaccgag gagagagacc acatcatcca gagcgagacc
      661 ctggaggccc acctgtaagc ggccgcaagc tttctagaga attcgatatc aagcttatcg
      721 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
      781 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
      841 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
      901 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
      961 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     1021 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     1081 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     1141 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     1201 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     1261 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg ctctcgagat
     1321 acgtacgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     1381 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     1441 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     1501 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     1561 ggcggaaaga tctaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     1621 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     1681 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     1741 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     1801 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     1861 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     1921 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     1981 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     2041 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     2101 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     2161 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     2221 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     2281 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     2341 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     2401 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     2461 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     2521 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2581 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2641 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2701 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2761 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2821 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2881 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2941 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3001 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3061 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3121 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3181 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3241 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3301 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3361 gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3421 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3481 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3541 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3601 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3661 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3721 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3781 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3841 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3901 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3961 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4021 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4081 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4141 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4201 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4261 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4321 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4381 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4441 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4501 attaatgcag ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg
     4561 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa
     4621 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctactcg
     4681 agaccggcgc gccgggggag gctgctggtg aatattaacc aaggtcaccc cagttatcgg
     4741 aggagcaaac aggggctaag tccacacgcg tggtaccgtc tgtctgcaca tttcgtagag
     4801 cgagtgttcc gatactctaa tctccctagg caaggttcat atttgtgtag gttacttatt
     4861 ctccttttgt tgactaagtc aataatcaga atcagcaggt ttggagtcag cttggcaggg
     4921 atcagcagcc tgggttggaa ggagggggta taaaagcccc ttcaccagga gaagccgtca
     4981 cacagatcca caagctcctg gtcgacacta gtaccggtgc caccatgaag tgggtaacct
     5041 ttctcctcct cctcttcgtc tccggctctg ctttttccag gggtgtgttt cgccgagaag
     5101 cacacaagag tgagatcgcc catcggtata atgatttggg agaacaacat ttcaaaggcc
     5161 tagtcctgat tgccttttcc cagtatctcc agaaatgctc atacgatgag catgccaaat
     5221 tagtgcagga agtaacagac tttgcaaaga cgtgtgttgc cgatgagtct gccgccaact
     5281 gtgacaaatc ccttcacact ctttttggag ataagttgtg tgccattcca aacctccgtg
     5341 aaaactatgg tgaactggct gactgctgta caaaacaaga gcccgaaaga aacgaatgtt
     5401 tcctgcaaca caaagatgac aaccccagcc tgccaccatt tgaaaggcca gaggctgagg
     5461 ccatgtgcac ctcctttaag gaaaacccaa ccacctttat gggacactat ttgcatgaag
     5521 ttgccagaag acatccttat ttctatgccc cagaacttct ttactatgct gagcagtaca
     5581 atgagattct gacccagtgt tgtgcagagg ctgacaagga aagctgcctg accccgaagc
     5641 ttgatggtgt gaaggagaaa gcattggtct catctgtccg tcagagaatg aagtgctcca
     5701 gtatgcagaa gtttggagag agagctttta aagcatgggc agtagctcgt ctgagccaga
     5761 cattccccaa tgctgacttt gcagaaatca ccaaattggc aacagacctg accaaagtca
     5821 acaaggagtg ctgccatggt gacctgctgg aatgcgcaga tgacagggcg gaacttgcca
     5881 agtacatgtg tgaaaaccag gcgactatct ccagcaaact gcagacttgc tgcgataaac
     5941 cactgttgaa gaaagcccac tgtcttagtg aggtggagca tgacaccatg cctgctgatc
     6001 tgcctgccat tgctgctgat tttgttgagg accaggaagt gtgcaagaac tatgctgagg
     6061 ccaaggatgt cttcctgggc acgttcttgt atgaatattc aagaagacac cctgattact
     6121 ctgtatccct gttgctgaga cttgctaaga aatatgaagc cactctggaa aagtgctgcg
     6181 ctgaagccaa tcctcccgca tgctacggca cagtgcttgc tgaatttcag cctcttgtag
     6241 aagagcctaa gaacttggtc aaaaccaact gtgatcttta cgagaagctt ggagaatatg
     6301 gattccaaaa tgccattcta gttcgctaca cccagaaagc acctcaggtg tcaaccccaa
     6361 ctctcgtgga ggctgcaaga aacctaggaa gagtgggcac caagtgttgt acacttcctg
     6421 aagatcagag actgccttgt gtggaagact atctgtctgc aatcctgaac cgtgtgtgtc
     6481 tgctgcatga gaagacccca gtgagtgagc atgttaccaa gtgctgtagt ggatccctgg
     6541 tggaaaggcg gccatgcttc tctgctctga cagttgatga aacatatgtc cccaaagagt
     6601 ttaaagctga gaccttcacc ttccactctg atatctgcac acttccagag aaggagaagc
     6661 agattaagaa acaaacggct cttgctgagc tggtgaagca caagcccaag gctacagcgg
     6721 agcaactgaa gactgtcatg gatgactttg cacagttcct ggatacatgt tgcaaggctg
     6781 ctgacaagga cacctgcttc tcgactgagg gtccaaacct tgtcactaga tgcaaagacg
     6841 ccttagcccc cgggggtgga ggcggttcag gcggaggtgg ctctggcggt ggcggatcag
     6901 tcgac
//
