LOCUS       AiP12723                4981 bp    DNA     circular UNA 14-OCT-2024
DEFINITION  pAAV-ExE0637m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   urn.local...i-iu11tiq
VERSION     urn.local...i-iu11tiq
KEYWORDS    .
SOURCE       (pAAV-eHGT_637m-minBglobin-SYFP2-WPRE-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1..141)
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     regulatory      156..899
                     /note="Geneious type: Enhancer"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AiE0637m"
     regulatory      906..958
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="minBG promoter"
     regulatory      993..1002
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Kozak sequence"
     CDS             999..1718
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SYFP2"
     3'UTR           1753..1998
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="WPRE3"
     misc_feature    2012..2215
                     /note="Geneious type: polyA_signal"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="BGH-pA"
     misc_feature    2244..2384
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     rep_origin      2476..2782
     regulatory      3196..3300
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     CDS             3301..4161
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     rep_origin      4309..4976
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtactca aatacctagt tcagtaccca
      181 gacactccca tacccttatt tagaagaaat aaatatcatc aagtcataat atccttgact
      241 gattaagaaa gccactttgt aagtgtttat taaactgtca agaaacttac agaatttact
      301 acatgatcgt tagaataact ttgagtcagg acatatttga tatgacttaa tcatactccc
      361 tccaaaagga aataaggctt tgtgaaggta aattatttct tcctgggttg gatatgtgtt
      421 tatggagtga tcattcagct gttcccaacc ttcattctga aaaggcctca gaacacttca
      481 tgatgaatca agctgtatcc tgaatagagt aaaatgaacc acttcgtagg aactatggtg
      541 tcaccacatc agcaattctt attgaaaagt gtgcatttct tattcacata tttcaaagat
      601 ggtattccag aggagtgatt ttctcaatgt atttttcatc tacaagcctt cattttaagc
      661 ctaccaccgt gtgtgttttc aagacagcaa ttatcgtttt aaaatgtgca ggtctagctt
      721 gagcttctca gcaagtttct atgccaaaga aaacaccaat cctttccatt tactgagaat
      781 caatgtttaa tcctcctttt tgttctcata cttattacaa atcataaaga attctgagtg
      841 tcagtttgat aactagaagc tccatgtacc attcctgctc cttattgatc attttgaatg
      901 agctcgggct gggcataaaa gtcagggcag agccatctat tgcttacatt tgcttctggg
      961 atccagatct ttcgaagcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc
     1021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     1141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     1201 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1441 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1501 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     1561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaagc
     1621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1681 ccgggatcac tctcggcatg gacgagctgt acaagtaagt cgacggcgcg ccgcggccgc
     1741 gaattcgata tcataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1801 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1861 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg
     1921 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     1981 gcactgacaa ttccgtggct cgagagatct tcgactgtgc cttctagttg ccagccatct
     2041 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2101 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2161 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgagatc
     2221 tcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     2281 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     2341 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     2401 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     2461 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2521 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2581 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2641 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     2701 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2761 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     2821 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2881 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     2941 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3001 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3061 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     3121 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3181 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3241 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3301 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3361 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3421 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3481 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3541 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3601 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3661 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3721 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     3781 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     3841 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     3901 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     3961 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4021 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4081 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4141 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4201 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4261 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4321 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4381 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4441 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     4501 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4561 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4621 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4681 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4741 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4801 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4861 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4921 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4981 t
//
