LOCUS       AiP12729                4848 bp    DNA     circular UNA 14-OCT-2024
DEFINITION  pAAV-ExE0580m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   urn.local...l-iu11tiq
VERSION     urn.local...l-iu11tiq
KEYWORDS    .
SOURCE       (pAAV-eHGT_580m-minBglobin-SYFP2-WPRE-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1..141)
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     regulatory      156..766
                     /note="Geneious type: Enhancer"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AiE0580m"
     regulatory      773..825
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="minBG promoter"
     regulatory      860..869
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Kozak sequence"
     CDS             866..1585
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SYFP2"
     3'UTR           1620..1865
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="WPRE3"
     misc_feature    1879..2082
                     /note="Geneious type: polyA_signal"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="BGH-pA"
     misc_feature    2111..2251
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     rep_origin      2343..2649
     regulatory      3063..3167
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     CDS             3168..4028
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     rep_origin      4176..4843
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatcta acatggctgc tatagcttac
      181 tgactagaag ttaagtgcac acttcctaaa agaaggcttt gacacaagcc acttcagttc
      241 cctcctcatt ttcttgtccc cattcctctc tctgtagaat tctgagattt caattcagtt
      301 ttatacagaa accacattac tgtaagccct acaaagttat ggcaatatag ctatatggag
      361 tcaagtaatg taggttattt ttttcccaat ggtgctggtg aaggtggcaa ttatgtagct
      421 atacttagca gactgaggaa attctgctag agtcagcatt tgtctcttca ttgctatgaa
      481 acagtaatgg aaaaataaac aaaaacaaaa ggcaaacact atgcataatt ccctcagatc
      541 atattaacat gtgatgttgg agtaaattgt tataacccca ttttggaaat acttacctta
      601 attaactatg atttccttaa aataatgcag tatttacaat ctatatgaaa gcactatatg
      661 ggacacatgg tatgatggaa cagtgcaccc aagagacacc aagaacattc ctgtctgtgg
      721 cagtcttttc tctatacaga ggcatttagt ctcaattgct cagagtgagc tcgggctggg
      781 cataaaagtc agggcagagc catctattgc ttacatttgc ttctgggatc cagatctttc
      841 gaagctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
      901 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      961 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
     1021 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcg tgcagtgctt
     1081 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1141 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1201 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1261 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1321 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1381 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1441 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     1501 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1561 cggcatggac gagctgtaca agtaagtcga cggcgcgccg cggccgcgaa ttcgatatca
     1621 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1681 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1741 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
     1801 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     1861 cgtggctcga gagatcttcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     1921 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     1981 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     2041 aggacagcaa gggggaggat tgggaagaca atagcaggca tgagatctca cgtgcggacc
     2101 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     2161 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     2221 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     2281 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     2341 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2401 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2461 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2521 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     2581 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2641 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     2701 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2761 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     2821 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2881 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2941 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3001 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3061 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3121 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3181 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3241 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3301 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3361 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3421 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3481 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3541 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3601 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3661 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3721 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3781 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3841 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3901 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3961 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4021 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4081 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4141 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4201 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4261 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4321 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4381 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4441 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4501 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4561 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4621 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4681 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4741 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4801 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
