LOCUS       AiP12730                4964 bp    DNA     circular UNA 14-OCT-2024
DEFINITION  pAAV-ExE0581m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   urn.local...m-iu11tiq
VERSION     urn.local...m-iu11tiq
KEYWORDS    .
SOURCE       (pAAV-eHGT_581m-minBglobin-SYFP2-WPRE-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1..130)
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     regulatory      129..790
                     /note="Geneious type: Enhancer"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AiE0581m"
     regulatory      895..947
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="minBG promoter"
     regulatory      982..991
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Kozak sequence"
     CDS             988..1707
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SYFP2"
     3'UTR           1742..1987
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="WPRE3"
     misc_feature    2001..2204
                     /note="Geneious type: polyA_signal"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="BGH-pA"
     misc_feature    2227..2367
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     rep_origin      2459..2765
     regulatory      3179..3283
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     CDS             3284..4144
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     rep_origin      4292..4959
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgctgtg ggtataggag gcatgtaaaa ttaatataat
      181 gttttgatat ttaggagaaa attgcaaaac aaaatgatga cagtgtttga aagtgtttga
      241 tcagtgccaa gcatcacttt atgtacttgg caaacatgac ttgaggcctt aagctgtgat
      301 ttgcaaatgt agattggaat caagatcttt atagatgagg aagcaaaaat cagaagacaa
      361 aataacatta tcaacttgat ctcatgtgca gccagggctg aactgcaaat gctgatttgc
      421 cccagtctgg gctcctcaaa tcgttccttg gaatcctatt agttggaact ttatctctgc
      481 tcgtggcagg gtgcctggga ccatgtttat aaatatctgc tgaatgaaga ataagtgagt
      541 caatcgaacc agaactcact ttggttagtt aatttcattc gtggtattta tggagagcag
      601 aagaaagaat tccagagaca cgatttgtca aaactctcta aagaaaatga tgacactata
      661 tattgatgaa aatgaatgtt cttgttcttg ctttatttga ttttcttgtc cccccactcc
      721 ccatctgcta gggtctcatt acagcatagt tcttgaatat cccaggttga cctgaagtta
      781 caatatattc ttgatttaga tggcagacat tgggaatatt ttgactctta aaatttaata
      841 gctaatcttt gaagaaaatt aaaatagtga tctccatttt gtagacaaga gctcgggctg
      901 ggcataaaag tcagggcaga gccatctatt gcttacattt gcttctggga tccagatctt
      961 tcgaagctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     1021 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1081 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     1141 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cgtgcagtgc
     1201 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1261 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1321 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1381 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1441 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     1501 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1561 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac
     1621 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1681 ctcggcatgg acgagctgta caagtaagtc gacggcgcgc cgcggccgcg aattcgatat
     1741 cataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     1801 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     1861 cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa
     1921 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     1981 tccgtggctc gagagatctt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2041 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     2101 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     2161 gcaggacagc aagggggagg attgggaaga caatagcagg catgcacgtg cggaccgagc
     2221 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     2281 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     2341 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     2401 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     2461 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     2521 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     2581 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     2641 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     2701 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     2761 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     2821 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     2881 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2941 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3001 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3061 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3121 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3181 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3241 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3301 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3361 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3421 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3481 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3541 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3601 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3661 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3721 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3781 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3841 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3901 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3961 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4021 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4081 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4141 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4201 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
