LOCUS       pSLB026_-_H16_A3514_in_ 6966 bp ds-DNA     circular     14-FEB-2025
DEFINITION  H16_A3514 in pET-28a(+)
COMMENT     Imported using the Genbank importer. File name: H16_A3514.gb
            Resistance markers: Kanamycin This file was created using tools
            provided by Twist Bioscience
FEATURES             Location/Qualifiers
     primer_bind     1..20
                     /label="T7"
     misc_binding    20..42
                     /label="LacO"
     misc_feature_   59..81
                     /label="RBS_3"
     CDS             88..1824
                     /translation="MGSSHHHHHHSSGLVPRGSHMMTRPVTPGLSVVAGSTDIPLSDETIPALLARTVAANPGAPAVVFREHGVRWSWAEFSDQVDRLASGLLRRGIQPGDRVGIWAPNRPEWLVTQFATARIGAILVNINPAYRRAELEYALNKAGVRMLITAARFKASDYLATLQALAPELAEAEPGALRSARLPRLQWVIRMGGEATPGMLNYDALLAEPDTVRLDAVTATLSALDAINIQFTSGTTGNPKGATLTHHNVVNNGRFVAIAMRLGDSDVLCIPVPLYHCFGMVLSVLACVSVGACMVFPGEAFDPLATMQAASEERCTALHGVPTMFIAQLDHPEFSRFDFSALRTGIMAGAPCPIEVMKRVVADMHMSEVTIAYGMTETSPVSFQSATDDPLDKRVATVGRVQPHLECKVVDALGEVVPTGATGELCTRGYSVMQGYWEDDERTREAIRDGWMHTGDLATIDDEGYCNIVGRVKDMLIRGGENIYPREIEEFLFRHPKVQAVQVFGVPDQKYGEEVCAWIVLKPGQSATEEEIRKFCRDQIAHYKIPRYIRFVSEMPMTVTGKVQKFVMRDTMIHDLGL*"
     misc_feature_   100..116
                     /label="6xHis"
     misc_feature_   151..1824
                     /label="H16_A3514"
     CDS             1831..1848
                     /label="6x His Tag"
     misc_feature_   2016..2322
                     /label="f1"
     CDS             complement(2549..3361)
                     /label="KanR"
     rep_origin      3407..4089
                     /label="ColE1 origin"
     misc_feature_   4498..4689
                     /label="ROP"
     misc_feature_   5464..5485
                     /label="CAP_binding"
     misc_feature_   5498..6589
                     /label="lacI"
     misc_feature_   6581..6658
                     /label="lacIQ_promoter"
ORIGIN
        1 TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT
       61 TGTTTAACTT TAAGAAGGAG ATATACCATG GGCAGCAGCC ATCATCATCA TCATCACAGC
      121 AGCGGCCTGG TGCCGCGCGG CAGCCATATG ATGACGCGGC CAGTGACCCC AGGACTTTCC
      181 GTAGTAGCCG GTTCTACCGA CATACCGCTG AGTGACGAAA CCATCCCAGC ACTTTTGGCG
      241 AGAACAGTAG CCGCCAACCC AGGAGCCCCG GCTGTGGTAT TCCGCGAGCA CGGCGTTAGA
      301 TGGAGCTGGG CTGAGTTCTC AGATCAGGTT GATAGATTAG CTAGCGGGCT TTTGAGACGA
      361 GGCATTCAGC CTGGCGACCG TGTAGGTATA TGGGCGCCCA ATCGGCCAGA GTGGTTGGTC
      421 ACTCAATTTG CCACTGCCCG GATAGGTGCT ATCTTGGTGA ACATTAACCC GGCTTATCGC
      481 CGAGCGGAGT TGGAATACGC TTTAAACAAG GCTGGTGTCC GAATGCTTAT AACAGCTGCC
      541 CGTTTCAAGG CTTCCGATTA CTTAGCAACC CTGCAGGCAT TGGCCCCTGA GCTTGCGGAA
      601 GCAGAGCCCG GTGCTTTACG GAGCGCCCGT CTTCCACGAT TGCAGTGGGT GATCCGGATG
      661 GGTGGTGAGG CTACTCCTGG GATGCTCAAC TACGACGCTC TTTTAGCAGA GCCTGACACA
      721 GTGCGGCTTG ATGCTGTGAC AGCAACATTG AGCGCATTGG ACGCCATCAA CATCCAGTTT
      781 ACATCCGGTA CCACGGGTAA TCCCAAGGGT GCTACTCTTA CACATCACAA CGTAGTTAAT
      841 AATGGACGCT TCGTTGCAAT AGCGATGAGA CTCGGGGATT CTGACGTCCT GTGCATTCCT
      901 GTTCCACTCT ACCACTGCTT TGGCATGGTA CTTAGCGTAC TTGCGTGCGT ATCCGTCGGA
      961 GCTTGTATGG TTTTCCCAGG CGAGGCGTTT GATCCCTTGG CTACCATGCA AGCCGCATCT
     1021 GAGGAACGCT GCACTGCTTT ACATGGCGTG CCCACAATGT TCATCGCACA GCTTGACCAC
     1081 CCCGAGTTCT CACGATTCGA CTTTTCTGCA TTGCGTACCG GGATAATGGC GGGTGCTCCG
     1141 TGTCCTATAG AGGTAATGAA GCGCGTGGTG GCTGATATGC ACATGAGTGA GGTGACTATC
     1201 GCTTACGGCA TGACTGAGAC CTCACCTGTA TCATTCCAAT CGGCGACAGA CGACCCTCTC
     1261 GACAAACGTG TAGCAACAGT GGGCAGAGTC CAACCTCATT TAGAGTGCAA AGTCGTCGAT
     1321 GCCCTGGGAG AAGTTGTTCC GACTGGGGCT ACCGGCGAGT TGTGCACACG AGGCTATAGT
     1381 GTTATGCAAG GCTATTGGGA AGACGACGAA CGAACCCGTG AAGCAATCAG AGACGGGTGG
     1441 ATGCACACCG GAGACCTGGC GACAATTGAT GACGAGGGCT ACTGCAATAT AGTTGGGCGC
     1501 GTCAAAGACA TGTTGATCCG TGGTGGTGAG AACATATATC CACGCGAAAT TGAGGAGTTT
     1561 CTTTTCCGTC ACCCGAAAGT GCAGGCGGTT CAGGTCTTCG GTGTCCCGGA CCAGAAGTAT
     1621 GGAGAGGAAG TATGCGCGTG GATAGTACTG AAACCAGGCC AATCTGCAAC AGAAGAGGAA
     1681 ATTCGTAAGT TCTGCCGGGA CCAAATAGCT CATTACAAGA TCCCCCGTTA TATACGGTTT
     1741 GTGTCGGAGA TGCCTATGAC TGTAACAGGA AAAGTGCAGA AGTTCGTGAT GCGAGACACA
     1801 ATGATCCACG ACTTGGGGCT CTGACTCGAG CACCACCACC ACCACCACTG AGATCCGGCT
     1861 GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA
     1921 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA
     1981 TCCGGATTGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG
     2041 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
     2101 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC
     2161 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG
     2221 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT
     2281 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG
     2341 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
     2401 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAGGTGG
     2461 CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA
     2521 TATGTATCCG CTCATGAATT AATTCTTAGA AAAACTCATC GAGCATCAAA TGAAACTGCA
     2581 ATTTATTCAT ATCAGGATTA TCAATACCAT ATTTTTGAAA AAGCCGTTTC TGTAATGAAG
     2641 GAGAAAACTC ACCGAGGCAG TTCCATAGGA TGGCAAGATC CTGGTATCGG TCTGCGATTC
     2701 CGACTCGTCC AACATCAATA CAACCTATTA ATTTCCCCTC GTCAAAAATA AGGTTATCAA
     2761 GTGAGAAATC ACCATGAGTG ACGACTGAAT CCGGTGAGAA TGGCAAAAGT TTATGCATTT
     2821 CTTTCCAGAC TTGTTCAACA GGCCAGCCAT TACGCTCGTC ATCAAAATCA CTCGCATCAA
     2881 CCAAACCGTT ATTCATTCGT GATTGCGCCT GAGCGAGACG AAATACGCGA TCGCTGTTAA
     2941 AAGGACAATT ACAAACAGGA ATCGAATGCA ACCGGCGCAG GAACACTGCC AGCGCATCAA
     3001 CAATATTTTC ACCTGAATCA GGATATTCTT CTAATACCTG GAATGCTGTT TTCCCGGGGA
     3061 TCGCAGTGGT GAGTAACCAT GCATCATCAG GAGTACGGAT AAAATGCTTG ATGGTCGGAA
     3121 GAGGCATAAA TTCCGTCAGC CAGTTTAGTC TGACCATCTC ATCTGTAACA TCATTGGCAA
     3181 CGCTACCTTT GCCATGTTTC AGAAACAACT CTGGCGCATC GGGCTTCCCA TACAATCGAT
     3241 AGATTGTCGC ACCTGATTGC CCGACATTAT CGCGAGCCCA TTTATACCCA TATAAATCAG
     3301 CATCCATGTT GGAATTTAAT CGCGGCCTAG AGCAAGACGT TTCCCGTTGA ATATGGCTCA
     3361 TAACACCCCT TGTATTACTG TTTATGTAAG CAGACAGTTT TATTGTTCAT GACCAAAATC
     3421 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
     3481 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
     3541 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC
     3601 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC
     3661 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT
     3721 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
     3781 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG
     3841 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA
     3901 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG
     3961 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA
     4021 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
     4081 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT
     4141 GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT
     4201 CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG
     4261 ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCAATGG TGCACTCTCA
     4321 GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA
     4381 CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
     4441 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA
     4501 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG
     4561 GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC
     4621 CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG
     4681 TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT
     4741 GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA
     4801 ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA
     4861 GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA
     4921 TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT
     4981 TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA
     5041 GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA
     5101 ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC
     5161 CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC
     5221 GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT
     5281 CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC
     5341 TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC
     5401 CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC
     5461 CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
     5521 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG
     5581 TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT
     5641 TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC
     5701 GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT
     5761 CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA
     5821 TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT
     5881 TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG
     5941 CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG
     6001 CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA
     6061 GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG
     6121 TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA
     6181 TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA
     6241 GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA
     6301 CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT
     6361 GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT
     6421 GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG
     6481 TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC
     6541 CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC
     6601 TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG
     6661 GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG
     6721 AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT
     6781 CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG
     6841 TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT
     6901 GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG
     6961 CGAAAT
//