LOCUS       Exported               12377 bp DNA     circular SYN 14-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12377)
  AUTHORS   David Gonzalez
  TITLE     Direct Submission
  JOURNAL   Exported Feb 17, 2025 from SnapGene Viewer 6.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..22
                     /label=RMLN
     misc_feature    50..322
                     /label=Alpha factor signal peptide
     CDS             50..316
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTDVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDSDVAVLPFSNGTNNRLLFINTTIASIAAKEEGVSLEKRGAEA"
     misc_feature    323..1816
                     /label=LaccID
     misc_feature    353..355
                     /label=mut
     misc_feature    455..457
                     /label=mut
     misc_feature    542..544
                     /label=mut
     misc_feature    683..685
                     /label=mut
     misc_feature    698..700
                     /label=mut
     misc_feature    1085..1087
                     /label=mut
     misc_feature    1220..1222
                     /label=mut
     CDS             1826..1849
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     misc_feature    1850..1858
                     /label=GSG linker
     CDS             1859..1882
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      1915..2162
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(1936..1955)
                     /label=RMLC
     rep_origin      complement(2410..2998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3169..4029)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(4125..4928)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4929..5144)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     protein_bind    complement(8044..8091)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(8244..9515)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     protein_bind    11108..11155
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      complement(11483..11938)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        join(11960..12377,1..24)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    11960..12077
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 cctctatact ttaacgtcaa ggagaaaaaa ctataggatc ataggatcca tgagatttcc
       61 ttcaattttt actgatgttt tattcgcagc atcctccgca ttagctgctc cagtcaacac
      121 tacaacagaa gatgaaacgg cacaaattcc ggctgaagct gtcatcggtt actcagattt
      181 agaaggggat tccgatgttg ctgttttgcc attttccaac ggcacaaata acaggttatt
      241 gtttataaat actactattg ccagcattgc tgctaaagaa gaaggggtat ctctcgagaa
      301 aagaggggct gaagctgaat tcgcgatagg acctgtggca gaccttcaca tcgccaatgc
      361 agacatcagc ccggatgggt tcactcgttc cgctgtgcta gcgggtggaa catttcccgg
      421 tcccttaata acgggtaata aaggagataa tttccggata aacgtcatca accagcttac
      481 ggacacgact atgttgaggt caaccagtat acattggcac ggattctttc aacacgggac
      541 aagttgggct gacggccccg ctttcgtcac tcagtgcccc atcgcaccgg gtaacagttt
      601 tctttacaac ttcactgtcc cggatcaagc gggcacgttc tggtatcata gtcacctaag
      661 tacccagtat tgtgacggac ttagtggtcc gtttgtagtt tacgacccca acgatcccca
      721 tcgtagctta tacgatgtgg atgatgaaag cactgtaatt actcttgcgg actggtacca
      781 caccccggct ccccagttgg ccgctgcgcc gcccgtccca gatagcaccc taataaacgg
      841 gaaaggaagg tatgccggag gcccaacgtc cccgttagcc gtgataaacg tagagcaggg
      901 aaaaagatat aggtttcgtc ttatatctat ttcctgtgat ccgaatttta catttagcat
      961 tgatggccat aatatgacta tcattgaagc tgatggggtg aacacgcaac cactgaccgt
     1021 ggactctatc cagattttcg ctggccaaag atattccttc gtcctgaatg cgaaccagcc
     1081 tgttggtaac tattggataa gagcgaatcc aaatatagga acgacaggat tcgcaggtgg
     1141 tattaatagt gcgatcctac gttatgtagg agctccagaa gcagacccta caacgaccca
     1201 aacaacatcc acaaatccag tgaacgaaac aaacctacac cccttggaga accccggagc
     1261 cccggggaat ccgactccgg gtggtgctga tgtgaacatc aaccttaaca tcggattctc
     1321 ctcaacggga caattcacca taaacggtgt ttccttcata ccacccaccg ttcccgtctt
     1381 gttgcaaatc ctgtccgggg cgcgtacggc acaagatctg cttcctagcg gctctgttta
     1441 tacattaccg ccgaacaaag tcatagaaat tagtatgcca ggcggcgcta gcgcagccgg
     1501 ggggcctcac ccgttccatt tgcacggcca cacttttgat gtggtacgtt cagcgggatc
     1561 atcaacttac aactacgtga atccagtcag aagagatgtc gtctccattg gaacggcggg
     1621 agataacgtg acaattaggt tcactacgga caacccgggt ccatggttcc tacattgtca
     1681 tatcgactgg catcttgagg ccggtttagc cgtcgtattc gctgaagaca cacctgatat
     1741 cgcaagcgct aatcccgttc cggctgcgtg ggacgaccta tgccctattt atgatgcgtt
     1801 aagccccgac gatcaagcgg ccgctttgga agttttgttt caaggtccag gttctggtca
     1861 tcaccatcac catcaccatc actaatgact cgagcatgca tctagagggc cgcatcatgt
     1921 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg
     1981 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
     2041 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
     2101 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
     2161 gcaagctgcg gccctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2221 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2281 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2341 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2461 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2521 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2581 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2641 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2701 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2761 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2821 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2881 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2941 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3001 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4021 aatactcata ctcttccttt ttcaatgggt aataactgat ataattaaat tgaagctcta
     4081 atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg
     4141 catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca
     4201 tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc
     4261 acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca
     4321 ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca
     4381 ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat
     4441 tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt
     4501 tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca
     4561 ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc
     4621 aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac
     4681 ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata
     4741 tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat
     4801 tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata
     4861 ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct
     4921 ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg ggttaagaat
     4981 actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg
     5041 tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga
     5101 aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta gcaggtacgt
     5161 agtatataga gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc
     5221 aacgctatca ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg
     5281 gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg
     5341 aagtggagtc aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa
     5401 ccttaacgga cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg
     5461 agaaaaaaag taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg
     5521 tagaacaaaa aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc
     5581 tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat
     5641 ttttgtttta caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc
     5701 atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt
     5761 tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt aacaaagata tgctattgaa
     5821 gtgcaagatg gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt acctatgcaa
     5881 tagatgcaat agtttctcca ggaaccgaaa tacatacatt gtcttccgta aagcgctaga
     5941 ctatatatta ttatacaggt tcaaatatac tatctgtttc agggaaaact cccaggttcg
     6001 gatgttcaaa attcaatgat gggtaacaag tacgatcgta aatctgtaaa acagtttgtc
     6061 ggatattagg ctgtatctcc tcaaagcgta ttcgaatatc attgagaagc tgcagcgtca
     6121 catcggataa taatgatggc agccattgta gaagtgcctt ttgcatttct agtctctttc
     6181 tcggtctagc tagttttact acatcgcgaa gatagaatct tagatcacac tgcctttgct
     6241 gagctggatc aatagagtaa caaaagagtg gtaaggcctc gttaaaggac aaggacctga
     6301 gcggaagtgt atcgtacagt agacggagta tactagtata gtctatagtc cgtggaattc
     6361 taagtgccag ctttataatg tcattctcct tactacagac ccgcctgaaa gtagacacat
     6421 catcatcagt aagctttgac aaaaagcatt gagtagctaa ctcttctatg caatctatag
     6481 ctgttttata aggcattcaa tggacagatt gaggtttttg aaacatacta gtgaaattag
     6541 ccttaatccc ttctcgaagt taatcatgca ttatggtgta aaaaatgcaa ctcgcgttgc
     6601 tctacttttt cccgaatttc caaatacgca gctggggtga ttgctcgatt tcgtaacgaa
     6661 agttttgttt ataaaaaccg cgaaaacctt ctgtaacaga tagattttta cagcgctgat
     6721 atacaatgac atcagctgta atggaaaata actgaaatat gaatggcgag agactgcttg
     6781 cttgtattaa gcaatgtatt atgcagcact tccaacctat ggtgtacgat gaaagtaggt
     6841 gtgtaatcga gacgacaagg gggacttttc cagttcctga caattataag aaatacaaaa
     6901 cgttagcatt tgcatttgtt ggacatgtac tgaatacaga cgacacaccg gtaattgaaa
     6961 aagaactgga ttggcctgat cctgcactag tgtacaatac aattgtcgat cgaatcataa
     7021 atcacccaga attatcacag tttatatcgg ttgcatttat tagtcagtta aaggccacca
     7081 tcggagaggg tttagatatt aatgtaaaag gcacgctaaa ccgcagggga aagggtatca
     7141 gaaggcctaa aggcgtattt tttagataca tggaatctcc atttgtcaat acaaaggtca
     7201 ctgcattctt ctcttatctt cgagattata ataaaattgc ctcagaatat cacaataata
     7261 ctaaattcat tctcacgttt tcatgtcaag catattgggc atctggccca aacttctccg
     7321 ccttgaagaa tgttattagg tgctccataa ttcatgaata catttctaag tttgtggaaa
     7381 gagaacagga taaaggtcat ataggagatc aggagctacc gcctgaagag gacccttctc
     7441 gtgaactaaa caatgtacaa catgaagtca atagtttaac ggaacaagat gcggaggcgg
     7501 atgaaggatt gtggggtgaa atagattcat tatgtgaaaa atggcagtct gaagcggaag
     7561 atcaaactga ggcggagata atagccgaca ggataattgg aaatagccag aggatggcga
     7621 acctcaaaat tcgtcgtaca aagttcaaaa gtgtcttgta tcatatacta aaggaactaa
     7681 ttcaatctca gggaaccgta aaggtttatc gcggtagtag tttttcacac gattcgataa
     7741 agataagctt acattatgaa gagcagcata ttacagccgt atgggtctac ttgacagtaa
     7801 aatttgaaga gcattggaag cctgttgatg tagaggtcga gtttagatgc aagttcaagg
     7861 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
     7921 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
     7981 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
     8041 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt
     8101 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
     8161 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
     8221 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
     8281 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
     8341 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
     8401 atcatttcct ttgatattgg atcatatgca tagtaccgag aaactagtgc gaagtagtga
     8461 tcaggtattg ctgttatctg atgagtatac gttgtcctgg ccacggcaga agcacgctta
     8521 tcgctccaat ttcccacaac attagtcaac tccgttaggc ccttcattga aagaaatgag
     8581 gtcatcaaat gtcttccaat gtgagatttt gggccatttt ttatagcaaa gattgaataa
     8641 ggcgcatttt tcttcaaagc tttattgtac gatctgacta agttatcttt taataattgg
     8701 tattcctgtt tattgcttga agaattgccg gtcctattta ctcgttttag gactggttca
     8761 gaattcctca aaaattcatc caaatataca agtggatcga tcctacccct tgcgctaaag
     8821 aagtatatgt gcctactaac gcttgtcttt gtctctgtca ctaaacactg gattattact
     8881 cccagatact tattttggac taatttaaat gatttcggat caacgttctt aatatcgctg
     8941 aatcttccac aattgatgaa agtagctagg aagaggaatt ggtataaagt ttttgttttt
     9001 gtaaatctcg aagtatactc aaacgaattt agtattttct cagtgatctc ccagatgctt
     9061 tcaccctcac ttagaagtgc tttaagcatt tttttactgt ggctatttcc cttatctgct
     9121 tcttccgatg attcgaactg taattgcaaa ctacttacaa tatcagtgat atcagattga
     9181 tgtttttgtc catagtaagg aataattgta aattcccaag caggaatcaa tttctttaat
     9241 gaggcttcca gaattgttgc tttttgcgtc ttgtatttaa actggagtga tttattgaca
     9301 atatcgaaac tcagcgaatt gcttatgata gtattatagc tcatgaatgt ggctctcttg
     9361 attgctgttc cgttatgtgt aatcatccaa cataaatagg ttagttcagc agcacataat
     9421 gctattttct cacctgaagg tctttcaaac ctttccacaa actgacgaac aagcacctta
     9481 ggtggtgttt tacataatat atcaaattgt ggcatgctta gcgccgatct tgtgtgcaat
     9541 tgatatctag tttcaactac tctatttatc ttgtatcttg cagtattcaa acacgctaac
     9601 tcgaaaaact aactttaatt gtcctgtttg tctcgcgttc tttcgaaaaa tgcaccggcc
     9661 gcgcattatt tgtactgcga aaataattgg tactgcggta tcttcatttc atattttaaa
     9721 aatgcacctt tgctgctttt ccttaatttt tagacggccc gcaggttcgt tttgcggtac
     9781 tatcttgtga taaaaagttg ttttgacatg tgatctgcac agattttata atgtaataag
     9841 caagaataca ttatcaaacg aacaatactg gtaaaagaaa accaaaatgg acgacattga
     9901 aacagccaag aatctgacgg taaaagcacg tacagcttat agcgtctggg atgtatgtcg
     9961 gctgtttatt gaaatgattg ctcctgatgt agatattgat atagagagta aacgtaagtc
    10021 tgatgagcta ctctttccag gatatgtcat aaggcccatg gaatctctca caaccggtag
    10081 gccgtatggt cttgattcta gcgcagaaga ttccagcgta tcttctgact ccagtgctga
    10141 ggtaattttg cctgctgcga agatggttaa ggaaaggttt gattcgattg gaaatggtat
    10201 gctctcttca caagaagcaa gtcaggctgc catagatttg atgctacaga ataacaagct
    10261 gttagacaat agaaagcaac tatacaaatc tattgctata ataataggaa gattgcccga
    10321 gaaagacaag aagagagcta ccgaaatgct catgagaaaa atggattgta cacagttatt
    10381 agtcccacca gctccaacgg aagaagatgt tatgaagctc gtaagcgtcg ttacccaatt
    10441 gcttacttta gttccaccag atcgtcaagc tgctttaata ggtgatttat tcatcccgga
    10501 atctctaaag gatatattca atagtttcaa tgaactggcg gcagagaatc gtttacagca
    10561 aaaaaagagt gagttggaag gaaggactga agtgaaccat gctaatacaa atgaagaagt
    10621 tccctccagg cgaacaagaa gtagagacac aaatgcaaga ggagcatata aattacaaaa
    10681 caccatcact gagggcccta aagcggttcc cacgaaaaaa aggagagtag caacgagggt
    10741 aaggggcaga aaatcacgta atacttctag ggtatgatcc aatatcaaag gaaatgatag
    10801 cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag ctaaagggta
    10861 gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag gaggtactag
    10921 actacctttc atcctacata aatagacgca tataagtacg catttaagca taaacacgca
    10981 ctatgccgtt cttctcatgt atatatatat acaggcaaca cgcagatata ggtgcgacgt
    11041 gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa
    11101 acgctttgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc
    11161 gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga
    11221 ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct caaaagtatc
    11281 tctttgctat atatctctgt gctatatccc tatataacct acccatccac ctttcgctcc
    11341 ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag tattactctt
    11401 tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa tacgaaaatg
    11461 taaacatttc ctatacgtag agaaattgta agcgttaata ttttgttaaa attcgcgtta
    11521 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    11581 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    11641 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    11701 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    11761 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    11821 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    11881 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg
    11941 ggatcgatcc actagtccac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga
    12001 agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct
    12061 cgcgccgcac tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga
    12121 ggaaaaattg gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac
    12181 cataggatga taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag
    12241 cgatgatttt tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact
    12301 aatactttca acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac
    12361 aaaaaattgt taatata
//
