LOCUS       pUE001-FtsZ1-Den        9908 bp    DNA     circular SYN 18-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9908)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Feb 18, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    267..625
                     /label=f1 ori
     primer_bind     complement(367..387)
                     /label=screening_pUE001_rev
     misc_feature    630..750
                     /label=p.fdx
     primer_bind     630..648
                     /label=seq_trpA_F
     primer_bind     complement(712..733)
                     /label=uvrB_dendra_rev_pTA231
                     /note="these primers do not bind in any construct and also 
                     not in uvrB. I don't why I ordered them or what i did with 
                     them??;;"
     primer_bind     714..733
                     /label=pTA231_uvrB_dendra_fwd
                     /note="to amplify pTA231 bb for the construction of the 
                     pUE001 construct (but i don't know why i used a different 
                     fwd primer here. The other bb fwd primers would have been 
                     better??);;"
     misc_feature    755..1588
                     /label=TrpA
     primer_bind     complement(1699..1718)
                     /label=seq_trpA_R
     promoter        1720..1824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1825..1893,1894..2685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2856..3444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3505..7252
                     /label=pHV2
     primer_bind     complement(3786..3799)
                     /label=uvrA_BamHI_RP
     primer_bind     7354..7373
                     /label=screening_pUE001_for
     promoter        join(7516..7521,7522..7539,7540..7546)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7554..7570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7662..7702
                     /label=uvrA_dendra_fwd_pTA231
                     /note="to amplify p.tna-uvrA-D2Hfx-t.syn from pTA962 
                     template and insert into pTA231 bb to create the pUE001 
                     plasmid;;"
     primer_bind     7662..7702
                     /label=FtsZ1_dendra_fwd_pTA231
                     /note="to amplify p.tna-ftsZ-D2Hfx-t.syn from pTA962 
                     template and insert into pTA231 bb to create the pUE001 
                     plasmid;;"
     primer_bind     7662..7702
                     /label=cas1_dendra_fwd_pTA231
                     /note="to amplify p.tna-cas1-D2Hfx-t.syn from pTA962 
                     template and insert into pTA231 bb to create the pUE001 
                     plasmid;;"
     primer_bind     complement(7664..7681)
                     /label=pTA231_assembly_rev
                     /note="to amplify pTA231 bb for the construction of the 
                     pUE001 construct;;"
     misc_feature    7682..7806
                     /label=L11e Terminator
     misc_feature    7815..8035
                     /label=p.tna
     primer_bind     complement(8021..8041)
                     /label=pTA962-Dendra2Hfx_rev
     primer_bind     complement(8022..8041)
                     /label=pUE001-Dendra2Hfx_rev
                     /note="to create pUE001-D2Hfx from any other pUE001 
                     construct and remove insert, e.g pUE001-FtsZ1:D2Hfx ;;"
     primer_bind     complement(8022..8041)
                     /label=pTA962-PAmCherry1Hfx_rev
     primer_bind     8022..8041
                     /label=pUE001-Dendra2Hfx_fwd
                     /note="to create pUE001-D2Hfx from any other pUE001 
                     construct and remove insert, e.g pUE001-FtsZ1:D2Hfx ;;"
     CDS             8039..9175
                     /codon_start=1
                     /label=ftsZ1
                     /translation="MDSIVGDAIDEAEAEDMGDESAQVDGAANINRSGTMTDDELKAVL
                     KDLQTNITVVGCGGAGGNTVNRMHEEGIKGAKLVAANTDVQHLVEIGADTKILMGEQKT
                     QGRGAGSLPQVGEEAALESQEEIYDAIEGSDMVFVTAGLGGGTGTGSAPVVAKAARESG
                     ALTIAIVTTPFTAEGEVRRTNAEAGLERLRDVSDTVIVVPNDRLLDAVGKLPVRQAFKV
                     SDEVLMRSVKGITELITKPGLVNLDFADVKTVMERGGVAMIGLGESDSESKAQESVKSA
                     LRSPLLDVDISGANSALVNVTGGSDMSIEEAEGVVEEIYDRIDPDARIIWGTSVDDELE
                     GMMRTMIVVTGVESPQIYGRNGEAQAHAEERLEDIDYVE"
     primer_bind     complement(8492..8501)
                     /label=bb_FtsZ_DendraM_HT7_rev
                     /note="creates pUE001-FtsZ1:D2Hfx:HT7 construct with 
                     methionine from pUE001-FtsZ1:D2hfx ;;"
     primer_bind     complement(8492..8501)
                     /label=bb_FtsZ_Dendra_HT7_rev
                     /note="creates pUE001-FtsZ1:D2Hfx:HT7 construct with stop 
                     codon from pUE001-FtsZ1:D2hfx ;;"
     primer_bind     complement(9158..9181)
                     /label=bb_FtsZ_HT7_rev
                     /note="to create backbone for pUE001-FtsZ1:HT7 from 
                     pUE001-FtsZ1:D2hfx ;;"
     primer_bind     9176..9193
                     /label=pTA962-Dendra2Hfx_fwd
     primer_bind     9176..9193
                     /label=uvrB_del_stop_for
                     /note="to remove STOP codon from pTA962-uvrB:D2Hfx and 
                     pUE001-uvrB:D2Hfx ;;"
     primer_bind     9176..9193
                     /label=uvrA_del_stop_for
                     /note="to remove STOP codon from pTA962-uvrA:D2Hfx and 
                     pUE001-uvrA:D2Hfx ;;"
     misc_feature    9176..9181
                     /label=linker
     misc_feature    9182..9871
                     /label=Dendra2Hfx
     primer_bind     9182..9199
                     /label=pUE001-Dendra2Hfx_fwd
                     /note="to create pUE001-D2Hfx from any other pUE001 
                     construct and remove insert, e.g pUE001-FtsZ1:D2Hfx ;;"
     primer_bind     complement(9257..9276)
                     /label=Sequencing_Dendra_rev
     primer_bind     complement(9850..9871)
                     /label=bb_FtsZ_Dendra_HT7_rev
                     /note="creates pUE001-FtsZ1:D2Hfx:HT7 construct with stop 
                     codon from pUE001-FtsZ1:D2hfx ;;"
     primer_bind     complement(9850..9868)
                     /label=bb_FtsZ_DendraM_HT7_rev
                     /note="creates pUE001-FtsZ1:D2Hfx:HT7 construct with 
                     methionine from pUE001-FtsZ1:D2hfx ;;"
     primer_bind     9872..9889
                     /label=bb_FtsZ_(Dendra)_HT7_for
                     /note="to create backbone for pUE001-FtsZ1:(D2Hfx):HT7 from
                     pUE001-FtsZ1:D2hfx ;;"
     misc_feature    9874..9908
                     /label=t.syn terminator
     primer_bind     complement(9878..9903)
                     /label=pTA231_screening_RP
     primer_bind     join(9889..9908,1..34)
                     /label=pTA231_uvrA_dendra_fwd
                     /note="to amplify pTA231 bb for the construction of the 
                     pUE001 construct (overhang to reverse primer of the 
                     insert);;"
     primer_bind     join(9889..9908,1..34)
                     /label=pTA231_FtsZ1_dendra_fwd
                     /note="to amplify pTA231 bb for the construction of the 
                     pUE001 construct (overhang to reverse primer of the 
                     insert);;"
     primer_bind     join(9889..9908,1..34)
                     /label=pTA231_cas1_dendra_fwd
                     /note="to amplify pTA231 bb for the construction of the 
                     pUE001 construct (overhang to reverse primer of the 
                     insert);;these are all the same primers. The pUE001 
                     constructs could have been created by only ordering these 
                     primers one time and just varying the pTA962 template."
     primer_bind     complement(9890..9908)
                     /label=uvrA_dendra_rev_pTA231
                     /note="to amplify p.tna-uvrA-D2Hfx-t.syn from pTA962 
                     template and insert into pTA231 bb to create the pUE001 
                     plasmid;;"
     primer_bind     complement(9890..9908)
                     /label=FtsZ1_dendra_rev_pTA231
                     /note="to amplify p.tna-ftsZ-D2Hfx-t.syn from pTA962 
                     template and insert into pTA231 bb to create the pUE001 
                     plasmid;;"
     primer_bind     complement(9890..9908)
                     /label=cas1_dendra_rev_pTA231
                     /note="to amplify p.tna-cas1-D2Hfx-t.syn from pTA962 
                     template and insert into pTA231 bb to create the pUE001 
                     plasmid;;"
     primer_bind     join(9904..9908,1..34)
                     /label=pTA231_uvrB_dendra_fwd
                     /note="to amplify pTA231 bb for the construction of the 
                     pUE001 construct (but i don't know why i used a different 
                     fwd primer here. The other bb fwd primers would have been 
                     better??);;"
ORIGIN
        1 aagcttggta ccgatatcga attcgatatc aagcttatcg ataccgtcga cctcgagggg
       61 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
      121 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      181 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      241 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      301 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      361 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      421 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      481 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      541 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      601 tatctcggtc tattcttttg atttagatcc cgtggataaa acccctcgtt gacgccgcgg
      661 gcagcgtcgt gatggcggtc acacccgggc tttcgtggca gtacgctggc ccgaacagca
      721 actactatgc gttcggaagc cgaactctgc agccatgtcg ctcgaagacg ccttctccga
      781 cggcccggcg ttcgtcccct atcttgccgc cggcgacccc gactacgagt cctcgctcga
      841 atacgtcgag gcgctcgaac gcggcggcgc ggacgtcatc gaactcggac ttccgttctc
      901 ggagcccatc gccgaggggc cgaccatcca gaacgcggtt gttcggtcgc tcgaaggcgg
      961 catgacgccg acgcgctttt tcgagttcgt cgaggacctc gacgtgtcgg tgccgctggt
     1021 ctgtatgagg tactacaacc tcatctatcg ctacggcgat gaacccggac cgcggccgtt
     1081 cgtcgagaag gcggcggaag tcggtatcga gggcttcgtc gtccccgacc tgccggccga
     1141 ggaggccggc ccgctccgcg aggcctgcga cgagttcggc ctcgacctcg tgttcatcgt
     1201 cgcgccgacg acccgcggcg agcgcctcga ccgaatcatg gaacaggtct cgggctacgt
     1261 ctacgtgcag gcgcgcctcg gcacgacggg cgcgcagtcg agcgtctccg accagaccga
     1321 ctcgtcgctc gaacgactca ccgactacga cgtgcccaag gcggtcggct tcggaatcag
     1381 cgacggcgac cacgccgagc gcatcgtcgc cagcggtgcc gacggcatca tcgtcggcag
     1441 cgcgctcgtc gacatcgtcg ccgagggcca cgagaacggc gacgacgccg aaacggtcgc
     1501 ggaccgactc gaaacgctcg cccgcgaact cgaagacggt gcggtagcgg gcgcgtcgca
     1561 acgcccaccg catccggaac gcacataact gctctagtaa gggattttgc cgatttcggc
     1621 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     1681 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1741 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1801 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1861 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1921 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1981 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2041 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2101 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2161 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2221 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2281 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2341 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2401 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2461 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2521 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2581 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2641 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2701 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2761 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2821 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2881 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2941 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3001 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3061 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3121 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3181 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3241 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3301 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3361 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3421 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3481 ttttgctggc cttttgctca catgagcttc tttgattcga gccatttgtt cctcgccggt
     3541 taggtgggag agttcgcccg attcgagtag ttggttcacc tcggggtaac tgagttcgtc
     3601 cggctcgcgc tcccgtaact cgtcgagcgg ctcgccccgt tttacattaa cctcgacctc
     3661 gacctcctcg accgaatacc cgcgctggag gtatgcctgt ctaacacgct cggcaatccc
     3721 ctcgaaagcg tcctttgcca aatcggacgg ttcgacggtg tcggtgatcc cgagatatag
     3781 gagagcgaac gaccacgaca ggacgtttag cgtatgctct ttcccgtcta aatcgccgtc
     3841 ctcgaaggct tccgttctaa ggtcctcgtc gagttccgaa accaggagcg gcatatcatg
     3901 tagggcttca tggagccgct cgactatcgc tcgccggcgt tcgtatcctg cctgtttgct
     3961 atactccttc ggtttttgga gataccggcg gtcggccggt gacaaaatcc ctcttgccct
     4021 ttcgtcaaga ctcatgcttt accaatcaca tcttcatgtc cgtaatgctt tatacgttgt
     4081 gccctaaagc gggatatatg tctgccacgg acacgcggat tccggtgtcg aaggatgtgc
     4141 ggcgcgacct ccgggttctc aaggcgcggg aaggccgacg gtcatacgat gagacaattg
     4201 cggtggtgct tgacgcatat ctctctgaga aggtggatta ataaactata gtcgtgacta
     4261 gcgaactagt tgaatcaaac gacgccggga gcacacgagt ctgccaggac ttcgctccta
     4321 tcggcgtcga aggatgctta cgcggacacg gcgggcgggt ggcccgttgt cctaactcaa
     4381 ccaatgtggt tggggcttat gggattatcg gattttcacc cggcgttagt gttcgcacaa
     4441 aagcgacact aaatgtacga tacggacgat acgacggcac acgaaccgac tgagaagcga
     4501 agccttcgct ttgcgactga cctcgaggcg gacgtctcgc ttacagactg gaccgccctt
     4561 cgggttcatc gagtaatctt cgacgagaca gagagtcaat ttgcgcttct cgaggcacta
     4621 caaacagcgg cggacggtgt cgacatactc gagtttgaac accgactgtc gagttgtggg
     4681 tttgacgtcg atgggtcagt cgtgaaactt gcacgacgac gtgtcgccgg cgagagacgg
     4741 gctctgtggg acttgattcg gtatgcgcga cggttgggac atgatgagat gggacagcca
     4801 cagagcgcct tagaggacgc tctcgcgttc actcgtctat tgggctatag caaccctcaa
     4861 cagacgatag caatcggtct caatgcggct ttcagagaga cgcggcgcga ccaggccgac
     4921 gagattatcg agtacgttct aacgctgtct gagagcgtcg acgtggtcct tggtgggtca
     4981 gagatcgacc gagtgtactt gcacaactac tacggcgata ttctcccatc gagtgttacg
     5041 aatccgtgca acgacacccg taatgattac tccattacga cgctcgtcga cgtggttggg
     5101 acggacggac ttgctgtcga gatacttcgg acggtaggtg agactgaaag cggacacatg
     5161 gcctacagcg ccctacaatc ggcgttcagt cagtacgata ggaccacggt ctcgcggcga
     5221 ttgaacgaga agctaatcga gtatggtctt tgtgagaagt acggcgctcg cggaaaccta
     5281 cgaatcgaga ttctcgacaa aggagcgttg tacctcgaag aattagccaa agagaaagag
     5341 cgacaaaagg cccttcagga ctacgttacg aacaccccaa aaccaaacca caatacccgt
     5401 gtaatcacgg accgccctcg gggagggcgg gcggacgacc accgaccgac cgccgaaggt
     5461 gaggcgtcga cgacgccgga cggacgggtt cgcttacctc ggtatcacgg tgtggactat
     5521 ctttcgtacg cagagacggt ctcagcggcg aagacaccgc cgaagaaagg cgttagtgtg
     5581 acaaactacc ctgtcgaacc caaatctgac cggggagaag ccgggtgggg ctaccttgag
     5641 gacgagaata cgctcgtcgt ctctgcagag tacgactctg ccatgtctca tgtggtgatg
     5701 attgcacgag cgttactcga tcctaagacg ttcgaccagg tccttaccga ggaccgtctc
     5761 gcggaacttg atggactaat cgaagatggg acgtacgttc gtgggtctcg aaacctcggg
     5821 tggttggctg actctgtcga ctcggcgggt gagtacgtcg acgtcctcga ggacgcgaga
     5881 gacgagctac tcgacatgac gcggtcactt gcacatgagg actacgagtg tgagacgtcc
     5941 gagtacctga gtcgaatcac gaaaacggcg atgggtctcg cgggaaccgc gtttcacgtc
     6001 ctcgatttac tcgatattga cgtcgtttgg gaggctcgct tacccgacta caaccgacac
     6061 ccggagcgat acggggagga caatgccgag ttgttagcaa cgacgcttgc gaagaacgcg
     6121 ccgattgcgg cgacgtacgg gaaccacgta gtccggcgac tgttgtttga agaccgagac
     6181 gagaagcgac gtcagtcgtt cgaccctgtc gtcgacgcga gcaatccgta tgcgaacctg
     6241 attgcgtcga tctcggtggt tggtgacttc gggaatcggg cggaccacgt cgccggcgta
     6301 atagaagacc gactgtcgaa ccctggggag attcacgagg acgcgccgga gattgcgctc
     6361 aaagtccctg tggtcgtcga gcatggtccg agtacggttg cacgagtaac acgggcaatg
     6421 tgtcgggcga aaggtctcga cgcgacgaga gacgcgatac ggctattctc tgggtttgca
     6481 cggacaccgt acgacgtagc ccacgcaatc gggcgagggt tgtctcaaga agcgacaccg
     6541 agagagattc ggagtagcga agtacggctt tcactcgcga gtctcccgtc gaagcgtctc
     6601 ttagaggacg caacccccac ggttcgggcg atgattagta cgttgctcgc gacgaatctg
     6661 agccttagca agacagagtt agccgagaaa gcgggaatct cgacgcagtc ggttcggaac
     6721 cacttaccga cacttgtagc aatggggtta gtcgacgaga ccgagggtca gtgccgactg
     6781 aacctttcgt tcacagacga gcaagaacgc cacaagcgga ttcttccatc gttcatgctg
     6841 accgagtcgt tcactcgagc gtcctctttg agacgctaga agcgcttgat gagttgaccg
     6901 aggaggtaat cgagatatgg gtacaatgcg gacctaacgg gctaccggac atcgaccgtc
     6961 tccatacgat gtacgactgg gtagactggg tacgtccctt actgtcgagt ctaaccgaag
     7021 aacgagacga gacggttcag acgctgtcta ttgggccgga gattgcacag caacccctca
     7081 cggaggcagg ttcgtgattg aactaacctt tggtaatatg ttcgtgatat gcgttgtatt
     7141 cgggtatctc gtaatctcgt ggtatctctc gagacagtac gttcatgatt agagtagggt
     7201 cgacgaactc tgaacctatg aatcgaggtc ggacaacaac cccccatgca tgttctttcc
     7261 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7321 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7381 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7441 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7501 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7561 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     7621 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgcg agctcggatc
     7681 cgacttcgac gactacttcg acgacgagta atcgtctcgg aaccgtctcg gcggttcgaa
     7741 ccgccctttc cgtcgggccg agtcgactcg gcccgctttc ttctctccac cgacccgatt
     7801 cgacccggta ccgggcccgt tctcgtcgcg ctctcgaagc tgtttctcgc gcgctcgcgt
     7861 cctcgaaagt gacatcgctc gaccggtggt cgtcggcggt cgctgaagtc ggctcgtggc
     7921 gagaacggaa cagccggcga caccgatgca cacaccagtc cacgagcgcc gaaaaccggg
     7981 cgtagcccct cgattttccg cctgccgatt acttcacatt cgcggaccta ttgcgcatat
     8041 ggactctatc gtcggcgacg caattgacga ggccgaggcc gaggacatgg gggatgagtc
     8101 ggctcaggtc gacggcgcgg ccaacatcaa ccggtccggg acgatgactg acgacgaact
     8161 gaaagcggtt ctcaaagacc tccagaccaa catcacggtg gtcgggtgcg gcggtgccgg
     8221 cggtaacacc gtcaaccgga tgcacgagga gggaatcaag ggggcgaagc tcgtcgccgc
     8281 caacaccgac gtgcagcacc tcgtggaaat cggggccgat acgaagattc tcatgggcga
     8341 gcagaagacc caaggccgcg gcgcgggctc gctcccgcag gtcggtgagg aggccgccct
     8401 cgaatcccaa gaggagattt acgacgccat cgagggctcc gacatggtgt tcgtcaccgc
     8461 cggactcggc ggcggcaccg gcaccggttc ggctcccgtc gtcgccaagg cggcccgcga
     8521 gtcgggcgcg ctcaccatcg ccatcgtcac gacgcccttt acggccgaag gcgaggtacg
     8581 acggacgaac gccgaggccg gtctcgaacg gctccgcgac gtgtcggaca cggtcatcgt
     8641 cgtcccgaac gaccgcctgc tcgacgccgt gggcaaactc cccgtccggc aggcgttcaa
     8701 ggtctccgac gaggtgctga tgcgctcggt caagggcatc accgaactca tcactaagcc
     8761 cggtctcgtc aacctcgact tcgccgacgt gaagaccgtc atggagcgcg gcggcgtcgc
     8821 catgatcggt ctcggcgagt ccgactccga gtccaaggct caggagtccg tcaagtccgc
     8881 cctccgctcg ccgcttcttg acgtggacat ctccggcgcg aactccgcgc tcgtcaacgt
     8941 caccggcggt tcggacatga gcatcgagga ggccgagggc gtcgtcgagg agatttacga
     9001 ccgcatcgac cccgacgcgc gcatcatctg ggggacctcc gtcgacgacg aactcgaagg
     9061 catgatgcgg acgatgatcg tcgtcaccgg cgtcgagtcg ccccaaatct acggccgcaa
     9121 cggcgaggca caggcgcacg ccgaagagcg tctcgaagac atcgactacg tcgagggatc
     9181 caacacgccg ggcatcaacc tcatcaaaga ggacatgcgc gtcaaggtcc acatggaagg
     9241 caacgtcaac ggccacgcgt tcgtcatcga aggcgaaggc aagggcaagc cgtacgaagg
     9301 cacgcagacg gcgaacctca cggtcaaaga aggcgccccg ctcccgttct cgtacgacat
     9361 cctcacgacg gcggtccact acggcaaccg cgtgttcacg aagtaccccg aggacatccc
     9421 ggactacttc aagcagtcgt tccccgaggg ctactcgtgg gagcgcacga tgacgttcga
     9481 ggacaagggc atctgcacga tccgctcgga catctcgctc gaaggcgact gcttcttcca
     9541 gaacgtccgc ttcaagggca cgaacttccc gccgaacggc ccggtcatgc agaaaaagac
     9601 gctcaagtgg gagccgtcga ccgagaagct ccacgtccgc gacggcctgc tcgtcggcaa
     9661 catcaacatg gcgctgctcc tcgaaggcgg cggccactac ctctgcgact tcaagacgac
     9721 gtacaaggcg aagaaggtcg tccagctccc ggacgcgcac ttcgtcgacc accgaatcga
     9781 gatcctcggc aacgactcgg actacaacaa agtcaagctc tacgagcacg cggtcgcgcg
     9841 atactcgccg ctcccgtcgc aagtctggta agcggccgca cctctggacc atcgcatttt
     9901 tcggcgcg
//
