LOCUS       pAAVEf1aFonConN2cG_2    7478 bp    DNA     circular SYN 04-FEB-2025
DEFINITION  pAAVEf1aFonConN2cG_2.
ACCESSION   pAAVEf1aFonConN2cG_2
VERSION     pAAVEf1aFonConN2cG_2
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     promoter        881..2059
                     /note="pLannotate"
                     /label="EF-1α promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    4875..5351
                     /note="pLannotate"
                     /label="hGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     repeat_region   5391..5531
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     promoter        6343..6447
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6448..7308
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    4255..4843
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      5606..6061
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    complement(2942..2975)
                     /note="pLannotate"
                     /label="loxN"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    2150..2183
                     /note="pLannotate"
                     /label="loxN"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(2858..2891)
                     /note="pLannotate"
                     /label="lox2272"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    2066..2099
                     /note="pLannotate"
                     /label="lox2272"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(3111..4055)
                     /note="pLannotate"
                     /label="G (fragment)"
                     /database="swissprot"
                     /identity="97.8"
                     /match_length="60.1"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2203..2847)
                     /note="pLannotate"
                     /label="G (fragment)"
                     /database="swissprot"
                     /identity="99.5"
                     /match_length="41.0"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(5536..5587)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(6062..6111)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             808..874
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6213..6248)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    4231..4255
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.1"
                     /fragment="True"
                     /other="misc_feature"
     repeat_region   662..689
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   718..745
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     intron          complement(4147..4197)
                     /note="pLannotate"
                     /label="β-globin intron (fragment)"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="10.7"
                     /fragment="True"
                     /other="intron"
     intron          complement(2892..2941)
                     /note="pLannotate"
                     /label="β-globin intron (fragment)"
                     /database="snapgene"
                     /identity="96.0"
                     /match_length="10.5"
                     /fragment="True"
                     /other="intron"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
      841 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
      901 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      961 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     1021 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     1081 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     1141 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     1201 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     1261 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     1321 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     1381 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     1441 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     1501 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     1561 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1621 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1681 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1741 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1801 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1861 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1921 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1981 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     2041 tccatttcag gtgtcgtgag gatccataac ttcgtatagg atactttata cgaagttatg
     2101 tagctggagc aatttgaaaa gattgttgta ttacctctag ctgcgcttaa taacttcgta
     2161 tagtatacct tatacgaagt tatgtcgacc ctcctgtact cacctgctat tggtaaaaat
     2221 gtcacaaggt gtccttggtc tcggattctc gggcatccaa atggtgtaat catggttagt
     2281 tgagcagtag gtagaggaca ccgttattcc tgagcacttt ccgccaggga agacccttga
     2341 gtgaagggat ttgtcatatg ggtccaaatc tgtcacactt ggggatatga taatgaggga
     2401 ctctttggtg gttcttacag ttcgaagcca gtggtagtcg gggtatggat tgtgtaggga
     2461 ctcttcatat ctggggtcac cggccatctt ccagttatac gcggctctac atgcgtctgg
     2521 ggtggggcgg aaatgctttc tcttgaatgt ggttgtgaca taaccaacaa agttggtgta
     2581 ggtctctgcc tctgtcacaa cacctgtgca agtgaacccg ttcactttga tggctgagat
     2641 gtatcccact ttgagttcca tgtaggagaa ctcggacagg ttggtacatc cttcatcctc
     2701 cacaaccagg ttatttggac agctgagatg gtgtatgtca atagggctcc agggaccaag
     2761 ttcgtctggt atcgtgtaaa tggggaactt cccgaaacac aacgaaaaac ccagaaggag
     2821 tacaaacaaa agaacctgag gaaccatggt ggctagcata acttcgtata aagtatccta
     2881 tacgaagtta tttgccttaa cccagaaatt atcactgtta ttctttagat aggtgcaaag
     2941 aataacttcg tataaggtat actatacgaa gttatggtac cgaagttcct attcttcaaa
     3001 tagtatagga acttcaattg ttattggtgc aaagaatcac ttccttaagt ctttatactg
     3061 cccaggaagt tcctattctt caaaaggtat aggaacttcg gcgcgcctta tagtctgatc
     3121 tcgcctccac tcttatatga ttcccatgaa ggtatgactt ttccgctttg ggaagtgact
     3181 gacacattcc cccctgtccc tccaaaactg cgttgtttcg attctggtcg attggctctt
     3241 ctgcaccatg tcattaggga aaatatcaac accaggccaa tcatggcccc tgcagtcatc
     3301 aatacatact ttccccagtt cgggagaccc aggtcaaccc ctgagatctg tttgtacaca
     3361 tcggggaggt gaacttcaac aaaatcctca gcctcatcac cttctttgaa aactgtagaa
     3421 gggtctgcca gggggtgcat cagggggata actgaagatt ccaacaactc catatgttgc
     3481 tggaggaggg atgattgcat ctctgggatt aggacatggt cgtcaggccc caatattata
     3541 ccattgaaaa acaccccgtt cacatgagga tggcaccttc ctccaacttt caaacaccct
     3601 tttgagggga tgatctcatt ccaggtccgg actgacttgt agtgagcatc agcctccatc
     3661 aaggttttgt tgaatatggt atatgctttt ccaaaccctg ggacaagttt tctcaggtga
     3721 ctgagacgtc tgaaacttac tgacttggtg gtcatgatgg actctaatgc gtccagacat
     3781 tcctctcttt tcttgactaa ctcctccaca acgagatgct caatctcgtc tgagcgaaag
     3841 tcgtgcaaat tcaccaactg atctggaggg caccatttgg tctcatctga tgtttgcatc
     3901 gcgacccatg ttccatccat aagtctaagt ccaagaactc cacataactt gagcctgcat
     3961 gctcctttta gagacttata caggcctctt tcatccacaa agccgcaagt cttgttcccg
     4021 ttggatgctc tcttccccct gtgggacgac atgacttaac cagttaacca tgttctctga
     4081 acatgagaag ggctggccac tctccatcta gagaagttcc tatactattt gaagaatagg
     4141 aacttcttgc cttaacccag aaattatcac tgttattctt tagaatagtg caaagagaag
     4201 ttcctatacc ttttgaagaa taggaacttc gaattcgata tcaagcttat cgataatcaa
     4261 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4321 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4381 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4441 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4501 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4561 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4621 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat
     4681 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4741 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4801 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg
     4861 ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     4921 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     4981 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     5041 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     5101 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     5161 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     5221 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     5281 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     5341 tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct
     5401 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     5461 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     5521 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     5581 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5641 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     5701 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5761 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5821 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5881 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     5941 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
     6001 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     6061 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     6121 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6181 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6241 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6301 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6361 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6421 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6481 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6541 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6601 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6661 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6721 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6781 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6841 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6901 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6961 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7021 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7081 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7141 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7201 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7261 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7321 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7381 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7441 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
