LOCUS       pCMV-evoCAST-TniQ_(pSL6 4782 bp ds-DNA     circular     18-FEB-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     Marker          complement(171..1031)
                     /label="amp marker"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="ApEinfo_revcolor: #85dae9 ApEinfo_fwdcolor: #85dae9 note: Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: amp marker Transferred_Similarity: 99.88% NCBI Feature Key: Marker"
     Promoter        complement(1073..1101)
                     /label="amp prom"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="ApEinfo_revcolor: #b7e6d7 ApEinfo_fwdcolor: #b7e6d7 note: Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: amp prom Transferred_Similarity: 100.00% NCBI Feature Key: Promoter"
     promoter        1422..2010
                     /label="CMV_Full"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="ApEinfo_revcolor: #c7b0e3 ApEinfo_fwdcolor: #c7b0e3 note: created_by: User modified_by: User Transferred_From: CMV_Full Transferred_Similarity: 99.83% NCBI Feature Key: promoter"
     Promoter        1929..2010
                     /label="CMV prom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="ApEinfo_revcolor: #b4abac ApEinfo_fwdcolor: #b4abac note: Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: CMV prom Transferred_Similarity: 97.56% NCBI Feature Key: Promoter"
     primer_bind     2031..2050
                     /label="T7"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="ApEinfo_revcolor: #85dae9 ApEinfo_fwdcolor: #85dae9 NCBI Feature Key: primer_bind"
     misc_feature    2087..3445
                     /label="2XbpNLS-PseTniQ"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2093..2146
                     /label="BP_NLS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="ApEinfo_revcolor: #ffef86 ApEinfo_fwdcolor: #ffef86 NCBI Feature Key: misc_feature"
     misc_feature    2157..2209
                     /label="BP_NLS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #ff9ccd ApEinfo_fwdcolor: #ff9ccd NCBI Feature Key: misc_feature"
     gene            2219..3442
                     /label="HCO PseTniQ"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: #b7e6d7 ApEinfo_fwdcolor: #b7e6d7 note: ApEinfo_revcolor: #b7e6d7 modified_by: thuang NCBI Feature Key: gene"
     Terminator      3494..3721
                     /label="bGH_PA term"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: #85dae9 ApEinfo_fwdcolor: #85dae9 note: Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: bGH_PA term Transferred_Similarity: 100.00% NCBI Feature Key: Terminator"
     misc_feature    complement(4179..16)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="ApEinfo_revcolor: #84b0dc ApEinfo_fwdcolor: #84b0dc note: Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: pBR322 origin Transferred_Similarity: 99.52% note: Geneious type: Origin of Replication NCBI Feature Key: misc_feature"
ORIGIN
        1 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACACTCAGT GGAACGAAAA CTCACGTTAA
       61 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
      121 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
      181 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
      241 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
      301 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
      361 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
      421 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
      481 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTAGTA TGGCTTCATT CAGCTCCGGT
      541 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
      601 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
      661 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCATAA GATGCTTTTC TGTGACTGGT
      721 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
      781 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
      841 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
      901 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
      961 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     1021 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     1081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA
     1141 TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCGATCTCC CGATCCCCTA
     1201 GGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT
     1261 GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA
     1321 GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGGGTTA GGCGTTTTGC GCTGCTTCGC
     1381 GATGTACGGG CCAGATATAC GCGTTGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA
     1441 ATTACGGGGT CATTAGTTCA TAGCCCATAT ATTGAGTTCC GCGTTACATA ACTTACGGTA
     1501 AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT
     1561 GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG
     1621 TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC
     1681 GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT
     1741 CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG
     1801 CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC
     1861 ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT
     1921 AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
     1981 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGAGAT CCGCGGCCGC TAATACGACT
     2041 CACTATAGGG AGACCCAAGC TTGGTACCGA GCTCGGATCC GCCACCATGG GCAAACGGAC
     2101 AGCCGACGGA AGCGAGTTCG AGTCACCGAA AAAGAAGCGA AAAGTTGGCT CCGGCAAAAG
     2161 AACCGCCGAC GGCAGCGAAT TCGAGTCACC CAAGAAGAAG AGGAAAGTCG GCTCCGGCGC
     2221 CTTCCTGTTT TCTCCCAAGG CCCGGGCCTT TAGCGACGAG TCCCTGGAGT CTTATCTGCT
     2281 GAGAGTGGTG AGCGAGAATT TCTTTGATAG CTACGAGGGC CTGTCCCTGG CCATCAGGGA
     2341 GGAGCTGCAC GAGCTGGACT TTGAGGCACA CGGAGCATTC CCAGTGGACC TGAAGCGGCT
     2401 GAACGTGTAC CACGCCAAGC ACAACTCTCA CTTCAGAATG AGAGCCCTGG GACTGCTGGA
     2461 GACCCTGCTG GACCTGCCCC GGTATGAGCT GCAGAAGCTG GCCCTGCTGA AGAGCGATAT
     2521 CAAGTTTAAC AGCTCCGTGG CCCTGTACAA CAATGGCGTG GACATCCCTC TGAGGTTCAT
     2581 CAGGCACCAC GCAGAGGAGG CAGTGGATTC TATCCCAGTG TGCAGCCAGT GTCTGGCCGA
     2641 GGAGGCCTAC ATCAAGCAGT CCTGGCACAT CAAGTGGGTG AATGCCTGCA CCAAGCACCA
     2701 GTGTGCCCTG CTGCACAACT GCCCCGAGTG TTACGCCCCT ATCAACTATA TCGAGAATGA
     2761 GTCCATCACA CACTGCTCTT GTGGCTTTGA GCTGAGCTGC GCCTCTACCA GCCCCGTGAA
     2821 TACACTGTCC ATCGAGCACC TGAACAAGCT GCTGGACAAG GGCGAGAGGA ACGATTCCAA
     2881 TCCTCTGTTT AACAATATGA CCCTGACAGA GAGGTTCGCC GCCCTGCTGT GGTATCAGGA
     2941 GCGGTACTCT CAGACCGACA ACTTCTGCCT GAATGATGCC GTGAACTACT TTAGCAAGTG
     3001 GCCTGCCGTG TTCAATACAG AGCTGGACGA GCTGTCCAAG AACGCCGAGA TGAAGCTGAT
     3061 CGATCTGTTT AACAAGACCG AGTTCAAGTT TATCTTCGGC GACGCCATCC TGGCCTGTCC
     3121 AAGCACACAG AAGCAGTCCG AGTCTCACTT CATCTACAGG GCCCTGCTGG ACTATCTGGT
     3181 GACCCTGGTG GAGTCCAATC CAAAGACAAA GAAGCCCAAT GCCGCCGATC TGCTGGTGTC
     3241 TGTGCTGGAG GCAGCCACCC TGCTGGGCAC AAGCGTGGAG CAGGTGTACC GCCTGTATCA
     3301 GAATGGCATC CTGCAGACCG CCTTTCGGCA CAAGATGAAT CAGAGAATCA ACCCTTACAA
     3361 GGGCGCCTTC TTTCTGAGGC ACGTGATCGA GTATAAGACA TCCTTCGGCA ACGATAAGGC
     3421 CCGCATGTAC CTGTCTGCCT GGTAACCGGT CATCATCACC ATCACCATTG AGTTTAAACC
     3481 CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC
     3541 GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA
     3601 ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC
     3661 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG
     3721 GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCGATACCGT CGACCTCTAG CTAGAGCTTG
     3781 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC
     3841 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTAGGGTG CCTAATGAGT GAGCTAACTC
     3901 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG
     3961 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT
     4021 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
     4081 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA
     4141 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
     4201 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
     4261 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
     4321 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
     4381 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
     4441 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
     4501 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
     4561 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
     4621 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
     4681 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
     4741 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AA
//