LOCUS       pCMV-evoCAST-evoTnsC_(p 4590 bp ds-DNA     circular     18-FEB-2025
DEFINITION  .
ACCESSION   urn.local...x04-8letjr8
VERSION     urn.local...x04-8letjr8
COMMENT     Imported using the Genbank importer. File name: 6 documents from
            CMV expression.gb 7016 TnsC-NLS codon optimized.
FEATURES             Location/Qualifiers
     Marker          complement(171..1031)
                     /label="amp marker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: amp marker Transferred_Similarity: 99.88%"
     Promoter        complement(1073..1101)
                     /label="amp prom"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: amp prom Transferred_Similarity: 100.00%"
     promoter        1422..2010
                     /label="CMV_Full"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="created_by: User modified_by: User Transferred_From: CMV_Full Transferred_Similarity: 99.83%"
     Promoter        1929..2010
                     /label="CMV prom"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: CMV prom Transferred_Similarity: 97.56%"
     Promoter        2031..2049
                     /label="T7 prom"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: T7 prom Transferred_Similarity: 100.00%"
     misc_feature    2063..3061
                     /label="HCO PseTnsC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             2063..3253
                     /label="evoPseTnsC-3xNLS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="created_by: thuang modified_by: thuang"
     Marker          3062..3250
                     /label="3xbpNLS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="created_by: thuang"
     Terminator      3302..3529
                     /label="bGH_PA term"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: bGH_PA term Transferred_Similarity: 100.00%"
     misc_feature    complement(3987..16)
                     /label="pBR322 origin"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="Imported_from: <ahref=""""http://wishart.biology.ualberta.ca/PlasMapper"""">PlasMapper</a> Transferred_From: pBR322 origin Transferred_Similarity: 99.52% note: Geneious type: Origin of Replication"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacActcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttAgta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccAtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     1201 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1261 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     1321 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     1381 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     1441 attacggggt cattagttca tagcccatat atTgagttcc gcgttacata acttacggta
     1501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1801 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1981 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
     2041 cactataggg agagccgcca ccatgaacgc cctgaccgag atccagatcg agaagctgag
     2101 gaaCttcagc gactgcatcg tgatgcaccc acagatcaag acaatcttca acgactttga
     2161 tgagctgagg ctgaatcgca agtttcagtc cgaccagcag tgtatgctgc tgatcggcga
     2221 taccggcgtg ggcaagtctc acacaatcaa ccactacaag aagcgggtgc tggccaccca
     2281 gaactattcc agaaatacaa tgcccgtgct ggtgtcccgg atcagccggg gcaagggcct
     2341 ggacgccacc ctggtgcaga tgctggccga tctggagctg ttcggcagct cccagatcaa
     2401 gaagcggggc tacaagaccg atctgacaaa gaagctggtg gagtctctga tcaaggccca
     2461 ggtggagctg ctgatcatca acgagttcca ggagctgatc gagtttaagt ctgtgcagga
     2521 gagacagcag atcgccaatg gcctgaagtt tatcagcgag gaggccaagg tgcctatcgt
     2581 gctggtgggc atgccatggg cagcaaagat cgcagaggag ccacagtggg cctctaggct
     2641 ggtgcggaag aTaaagctgg agtatttcag cctgaagaac gactccaagt actttcgcca
     2701 gtatctgatg ggcctggcca agaagatgcc tttcgatgtg ccccctaagc tggagagcaa
     2761 gaacaccaca atcgccctgt ttgcagcatg caggggagag aatcgcgccc tgaagcacct
     2821 gctgctggag gccctgaagc tggccctgag ctgtaacgag tacctggaga ataagcactt
     2881 catcaccgcc tatgacaagt ttgatttctt taacgacaag gagaagctga agtccaagaa
     2941 tcctttcaag caggacatca aggatatcga aatctacgaa gtgatcaaga acagcagcta
     3001 taaAccaaat gccctggacc ccgaggatat gctgacagat cgggtgtttg ccatcgtgaa
     3061 gggctccggc aagcggactg ctgatggcag tgaatttgag tccccaaaga agaagagaaa
     3121 ggtggggagc ggcaaaagaa ccgccgacgg cagcgaattc gagtcaccca agaagaagag
     3181 gaaagtcggc tccggaaaac ggacagccga cggaagcgag ttcgagtcac cgaaaaagaa
     3241 gcgaaaagtt taaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     3301 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3361 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3421 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3481 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     3541 gaaagaacca gctggggctc gataccgtcg acctctagct agagcttggc gtaatcatgg
     3601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3661 ggaagcataa agtgtaaagc ctagggtgcc taatgagtga gctaactcac attaattgcg
     3721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4561 cagattacgc gcagaaaaaa aggatctcaa
//