LOCUS       pCMV-PuroR-T2A-eGFP_(pS 5755 bp ds-DNA     circular     18-FEB-2025
DEFINITION  pSL2268.
ACCESSION   pSL2268
VERSION     pSL2268
COMMENT     Imported using the Genbank importer. File name:
            T4W6PR_8_pSL2268.gbk Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    877..898
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        913..943
                     /label="lac promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /label="lac operator"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        1012..1030
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1449..1560)
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     misc_feature    complement(1585..2967)
                     /label="PuroR-T2A-eGFP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(1594..2307)
                     /label="EGFP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             complement(2308..2370)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     CDS             complement(2371..2967)
                     /label="PuroR (fragment)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="99.7"
                     /fragment="True"
                     /other="CDS"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(3051..3254)
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(3255..3634)
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(3953..3971)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(4138..4593)
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        4620..4724
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    4725..5585
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgg ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tgggtaccgg gcccgtttaa actacagttg aagtcggaag
     1081 tttacataca ccttagccaa atacatttaa actcactttt tcacaattcc tgacatttaa
     1141 tccgagtaaa gattccctgt cttaaggtca gttaggatca ccactttatt ttaagaatgt
     1201 gaaatatcag aataatagta gagagaatga ttcatttcag cttttatttc tttcatcaca
     1261 ttcccagtgg gtcagaagtg tacatacacg cgcttagtat ttggtagcat tgcctttaaa
     1321 ttgtttaaag atctggccat ctagacagta agtccccagc atgcctgcta ttctcttccc
     1381 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     1441 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     1501 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     1561 tcgaggctga tcagcgagct ctagttagaa ttccttgtac agctcgtcca tgccgagagt
     1621 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
     1681 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
     1741 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
     1801 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
     1861 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
     1921 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
     1981 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
     2041 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
     2101 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
     2161 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
     2221 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
     2281 ggtgaacagc tcctcgccct tgctcactgg gccaggattc tcctcgacgt caccgcatgt
     2341 tagcagactt cctctgccct ctccactgcc ggcaccgggc ttgcgggtca tgcaccaggt
     2401 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     2461 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     2521 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     2581 tccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     2641 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     2701 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     2761 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     2821 cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta
     2881 gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag
     2941 gcgcaccgtg ggcttgtact cggtcatggt ggctaccggt aagcttcgtg atgtccagtg
     3001 ctattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt
     3061 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     3121 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     3181 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     3241 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     3301 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     3361 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     3421 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     3481 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     3541 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     3601 gattactatt aataactagt caataatcaa tgtcaattaa ttcgcggccg cgcgcactag
     3661 tgaattccat ggatatcaag cttaaaggca cagtcaagcg cgtgtatgta cacttctgac
     3721 ccactggaat tgtgatacag tgaattataa gtgaaataat ctgtctgtaa acaattgttg
     3781 gaaaaatgac ttgtgtcatg cacaaagtag atgtcctaac tgacttgcca aaactattgt
     3841 ttgttaacaa gaaatttgtg gagtagttga aaaacgagtt ttaatgactc caacttaagt
     3901 gtatgtaaac ttccgacttc aactgtaggt ttaaacgcta gctccaattc gccctatagt
     3961 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4021 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4081 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa
     4141 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4201 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4261 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4321 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4381 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4441 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4501 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccactc
     4561 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     4621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4681 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4741 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4801 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4861 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4921 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4981 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5041 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5101 ccatgagtga taacactgcg gccaagttac ttctgacaac gatcggagga ccgaaggagc
     5161 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5221 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5281 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5341 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5401 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5461 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5521 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5581 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5641 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5701 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//