LOCUS       3485_pLV.tetO.As        9798 bp DNA     circular SYN 09-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 20, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..16
                     /label=cppt
                     /label=cppt(1)
     misc_feature    154..471
                     /label=TRE
     misc_feature    618..1313
                     /label=ms Ascl1
     misc_feature    1013
                     /label=c in seq
     misc_feature    1374..1601
                     /gene="U6"
                     /label=U6
                     /label=U6(1)
     misc_feature    1624..1644
                     /label=REST anti sense
     misc_feature    1645..1652
                     /label=loop
     misc_feature    1653..1673
                     /label=REST sense
     misc_feature    1674..1678
                     /label=U6 terminator
                     /label=U6 terminator(1)
     misc_feature    1716..1943
                     /gene="U6"
                     /label=U6
     misc_feature    1966..1986
                     /label=REST anti sense
                     /label=REST anti sense(1)
     misc_feature    1987..1994
                     /label=loop
                     /label=loop(1)
     misc_feature    1995..2015
                     /label=REST sense
                     /label=REST sense(1)
     misc_feature    2016..2020
                     /label=U6 terminator
     misc_feature    2042..2636
                     /label=WPRE*
                     /note="86bp downstream SacII-site removed by linker insert 
                     (partial homology retained though)"
                     /note="/vntifkey=21"
     misc_feature    2823..2838
                     /label=cppt
ORIGIN
        1 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
       61 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      121 tattacaggg acagcagaga tccagtttgg ttactcgagt ttaccactcc ctatcagtga
      181 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      241 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      301 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
      361 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
      421 ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga gtaggcgtgt
      481 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc
      601 cgaattcgct agccaccatg gagagctctg gcaagatgga gagtggagcc ggccagcagc
      661 cgcagccccc gcagcccttc ctgcctcccg cagcctgctt ctttgcgacc gcggcggcgg
      721 cggcagcggc ggcggccgcg gcagctcaga gcgcgcagca gcaacagccg caggcgccgc
      781 cgcagcaggc gccgcagctg agcccggtgg ccgacagcca gccctcaggg ggcggtcaca
      841 agtcagcggc caagcaggtc aagcgccagc gctcgtcctc tccggaactg atgcgctgca
      901 aacgccggct caacttcagc ggcttcggct acagcctgcc acagcagcag ccggccgccg
      961 tggcgcgccg caacgagcgc gagcgcaacc gggtcaagtt ggtcaacctg ggttttgcca
     1021 ccctccggga gcatgtcccc aacggcgcgg ccaacaagaa gatgagcaag gtggagacgc
     1081 tgcgctcggc ggtcgagtac atccgcgcgc tgcagcagct gctggacgag cacgacgcgg
     1141 tgagcgctgc ctttcaggcg ggcgtcctgt cgcccaccat ctcccccaac tactccaacg
     1201 acttgaactc tatggcgggt tctccggtct cgtcctactc ctccgacgag ggatcctacg
     1261 accctcttag cccagaggaa caagagctgc tggactttac caactggttc tgagaattcg
     1321 atatcaagct tatcgatacg agactagcct cgagcggccg cccccttcac cgagggccta
     1381 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
     1441 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     1501 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     1561 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     1621 ggtacttcaa taaggacttg ctccacacgt gaggagcaag tccttattga agttttttga
     1681 attctcgacc ggatcccggc cgcccccttc accgagggcc tatttcccat gattccttca
     1741 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     1801 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     1861 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     1921 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggtagtac acattaacca
     1981 aatggcacac gtgagccatt tggttaatgt gtactttttt gaattctcga cctcgagaag
     2041 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2101 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2161 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2221 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2281 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2341 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2401 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2461 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2521 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2581 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     2641 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     2701 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     2761 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2821 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     2881 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     2941 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     3001 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     3061 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     3121 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     3181 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3241 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3301 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     3361 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3421 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3481 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3541 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3601 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3781 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3841 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3901 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3961 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4021 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4081 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4141 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4201 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4261 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4321 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4381 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4441 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     4501 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     4561 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     4621 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     4681 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     4741 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     4801 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     4861 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     4921 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     4981 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5161 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5221 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5281 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5341 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5401 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5461 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5521 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5581 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5641 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5701 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5761 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5821 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5881 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5941 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6001 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6061 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6121 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6301 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6361 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6421 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6481 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6541 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6601 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6661 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6721 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6781 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6841 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6901 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6961 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7021 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7081 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7141 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7201 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7261 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7321 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     7381 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     7441 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     7501 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     7561 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     7621 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     7681 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     7741 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     7801 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     7861 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     7921 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     7981 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     8041 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     8101 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     8161 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     8221 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8281 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8341 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     8401 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     8461 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     8521 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8581 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8641 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8701 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     8761 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     8821 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     8881 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8941 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     9001 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     9061 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     9121 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     9181 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     9241 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     9301 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     9361 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     9421 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9481 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9541 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9601 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9661 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     9721 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     9781 gtattcatcc acaatttt
//
