LOCUS       3412_pLV.tetO.Lm        9601 bp DNA     circular SYN 03-MAY-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 21, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..16
                     /label=cppt
                     /label=cppt(1)
     misc_feature    154..471
                     /label=TRE
     misc_feature    614..740
                     /label=Mus musculus Lmx1a
     misc_feature    741..1762
                     /label=Mus musculus Lmx1a
                     /label=Mus musculus Lmx1a(1)
     misc_feature    1852..2439
                     /label=WPRE
     misc_feature    2626..2641
                     /label=cppt
ORIGIN
        1 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
       61 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      121 tattacaggg acagcagaga tccagtttgg ttactcgagt ttaccactcc ctatcagtga
      181 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      241 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      301 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
      361 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
      421 ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga gtaggcgtgt
      481 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc
      601 cgaattcgct agcatgttgg acggcctgaa gatggaggag aactttcaaa gtgcgattga
      661 gacctcggca tctttctcct ctttgctggg cagagcggtg agccccaagt ctgtctgcga
      721 gggctgtcag cgggtcatct cggacaggtt tctgctgcgg ctcaacgaca gcttctggca
      781 cgagcaatgc gtgcagtgtg cctcctgcaa agagcccctg gagaccacct gcttctaccg
      841 ggacaagaag ctctactgca agtaccacta cgagaaactg tttgctgtca aatgtggggg
      901 ctgcttcgag gccattgcgc ccaatgagtt tgtcatgcgt gcccagaaga gcgtatacca
      961 cctgagctgc ttctgctgct gcgtctgtga gcgacagctg cagaagggtg acgagtttgt
     1021 cctgaaggag ggccagctgc tctgcaaagg ggactatgag aaagaacggg agctgctgag
     1081 cctggtgagc cctgcggcct cagactcagg caaaagcgat gatgaggaga gcctttgcaa
     1141 gtcagcccat ggggcaggaa aaggagcatc agaggacggc aaggaccata agcgacccaa
     1201 acgtcccaga accatcctga ccactcagca gaggagagca ttcaaggcct cgtttgaagt
     1261 atcctccaag ccctgcagaa aggtgaggga gactctggct gcagagacag ggctgagtgt
     1321 ccgtgtggtt caggtatggt tccagaacca gcgagccaag atgaagaagc tggcccggcg
     1381 acagcagcaa cagcaacagg accaacagaa cacccagagg ctgacttctg ctcagacaaa
     1441 tggtagtggg aatgcgggca tggaagggat catgaacccc tatacaacat tgcccacccc
     1501 acagcagctg ctggccattg aacagagcgt ctacaactct gatcccttcc gacagggtct
     1561 caccccaccc cagatgcctg gagatcacat gcacccctat ggtgctgaac ctcttttcca
     1621 tgacttggat agtgatgaca catctctcag taacctggga gactgcttcc tggcaacctc
     1681 agaagctggg cctctgcagt ccagagtggg aaaccccatt gaccatctgt actccatgca
     1741 gaattcctat ttcacctctt gaggatccta cgaccctctt agcccagagg aacaagagct
     1801 gctggacttt accaactggt tctgagaatt cgatatcaag cttatcgata atcaacctct
     1861 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     1921 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     1981 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2041 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2101 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2161 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2221 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     2281 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     2341 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     2401 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga
     2461 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     2521 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     2581 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     2641 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     2701 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     2761 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     2821 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     2881 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     2941 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     3001 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     3061 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     3121 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     3181 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     3241 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     3301 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     3361 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     3421 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3481 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3541 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3601 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3661 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3721 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3781 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3841 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     3901 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3961 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4021 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4081 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4141 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4201 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     4261 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     4321 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     4381 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     4441 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     4501 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     4561 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     4621 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     4681 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     4741 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     4801 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     4861 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4921 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     4981 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5041 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5101 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5161 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5221 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5281 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5341 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5401 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5461 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5521 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5581 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5641 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5701 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5761 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5821 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5881 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5941 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6001 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6061 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6121 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6181 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6241 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6301 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6361 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6421 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6481 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6541 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6601 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6661 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6721 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6781 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6841 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6901 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6961 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7021 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7081 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7141 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     7201 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     7261 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     7321 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     7381 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     7441 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     7501 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     7561 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     7621 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     7681 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     7741 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     7801 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     7861 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     7921 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
     7981 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8041 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8101 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8161 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     8221 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     8281 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     8341 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     8401 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     8461 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     8521 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     8581 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     8641 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     8701 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     8761 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     8821 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     8881 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     8941 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     9001 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     9061 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     9121 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     9181 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     9241 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     9301 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     9361 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     9421 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     9481 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     9541 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     9601 t
//
