LOCUS       3423_pLV.tetO.Nu       10249 bp DNA     circular SYN 17-MAY-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 21, 2025 from SnapGene 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..16
                     /label=cppt
                     /label=cppt(1)
     misc_feature    154..471
                     /label=TRE
     misc_feature    614..2410
                     /label=Nurr1
     misc_feature    2500..3087
                     /label=WPRE
     misc_feature    3274..3289
                     /label=cppt
     rep_origin      4110..4416
                     /label=F1 ori
     primer_bind     complement(5648..5668)
                     /label=M13-rev
     misc_binding    complement(5674..5696)
                     /label=LacO
     rep_origin      complement(6036..6718)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6816..7475)
                     /label=AmpR
ORIGIN
        1 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
       61 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      121 tattacaggg acagcagaga tccagtttgg ttactcgagt ttaccactcc ctatcagtga
      181 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      241 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      301 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
      361 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
      421 ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga gtaggcgtgt
      481 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc
      601 cgaattcgct agcatgcctt gtgttcaggc gcagtatggg tcctcgcctc aaggagccag
      661 ccccgcttct cagagctaca gttaccactc ttcgggagaa tacagctccg atttcttaac
      721 tccagagttt gtcaagttta gcatggacct caccaacact gaaattactg ccaccacttc
      781 tctccccagc ttcagtacct ttatggacaa ctacagcaca ggctacgacg tcaagccacc
      841 ttgcttgtac caaatgcccc tgtccggaca gcagtcctcc attaaggtag aagacattca
      901 gatgcacaac taccagcaac acagccacct gccccctcag tccgaggaga tgatgccaca
      961 cagcgggtcg gtttactaca agccctcttc gcccccgaca cccagcaccc cgagcttcca
     1021 ggtgcagcat agcccgatgt gggacgatcc gggctccctt cacaacttcc accagaacta
     1081 cgtggccact acgcatatga tcgagcagag gaagacacct gtctcccgcc tgtcactctt
     1141 ctcctttaag cagtcgcccc cgggcactcc tgtgtctagc tgccagatgc gcttcgacgg
     1201 gcctctgcac gtccccatga acccggagcc cgcgggcagc caccacgtag tggatgggca
     1261 gaccttcgcc gtgcccaacc ccattcgcaa gccggcatcc atgggcttcc cgggcctgca
     1321 gatcggccac gcatcgcagt tgcttgacac gcaggtgccc tcgccgccgt cccggggctc
     1381 tccctccaat gagggtctgt gcgctgtttg cggtgacaac gcggcctgtc agcactacgg
     1441 tgttcgcact tgtgagggct gcaaaggttt ctttaagcgc acggtgcaaa aaaacgcgaa
     1501 atatgtgtgt ttagcaaata aaaactgccc agtggacaag cgccgccgaa atcgttgtca
     1561 gtactgtcgg tttcagaagt gcctagctgt tgggatggtt aaagaagtgg ttcgcacgga
     1621 cagtttaaaa ggccggagag gtcgtttacc ctcgaagccg aagagcccac aggatccctc
     1681 tcccccctca cctccggtga gtctgatcag tgccctcgtc agagcccacg tcgattccaa
     1741 tccggcaatg accagcctgg actattccag gttccaggca aaccctgact atcagatgag
     1801 tggagatgat acccaacata tccagcagtt ctacgatctc ctgaccggct ctatggagat
     1861 catcagaggg tgggcagaga agatccctgg ctttgctgac ctgcccaaag ccgaccagga
     1921 cctgcttttt gaatcagctt tcttagaatt atttgttctg cgcttagcat acaggtccaa
     1981 cccagtggag ggtaaactca tcttttgcaa tggggtggtc ttgcacaggt tgcaatgcgt
     2041 gcgtggcttt ggggaatgga ttgattccat tgttgaattc tcctccaact tgcagaatat
     2101 gaacatcgac atttctgcct tctcctgcat tgctgccctg gctatggtca cagagagaca
     2161 cgggctcaag gaacccaaga gagtggaaga gctacaaaac aaaattgtaa attgtcttaa
     2221 agaccatgtg actttcaata atgggggttt gaaccgaccc aactacctgt ctaaactgtt
     2281 ggggaagctg ccagaactcc gcaccctttg cacacagggc ctccagcgca ttttctacct
     2341 gaaattggaa gacttggtac caccaccagc aataattgac aaacttttcc tggacacctt
     2401 acctttctaa ggatcctacg accctcttag cccagaggaa caagagctgc tggactttac
     2461 caactggttc tgagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     2521 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     2581 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     2641 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     2701 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     2761 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     2821 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     2881 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     2941 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3001 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3061 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     3121 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     3181 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     3241 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     3301 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     3361 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     3421 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     3481 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     3541 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     3601 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     3661 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3721 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3781 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     3841 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3901 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3961 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4021 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4081 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4141 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4201 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4261 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4321 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4381 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4441 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4501 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4561 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4621 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4681 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4741 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4801 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4861 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     4921 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     4981 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     5041 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     5101 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     5161 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     5221 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     5281 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     5341 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5401 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5461 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     5521 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5581 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     5641 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5701 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5761 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5821 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5881 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5941 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6001 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6061 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6121 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6181 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6241 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6301 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6361 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6421 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6481 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6541 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6601 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6661 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6721 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6781 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6841 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6901 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6961 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7021 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7081 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7141 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7201 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7261 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7321 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7381 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7441 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7501 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7561 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7621 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7681 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7741 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7801 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     7861 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     7921 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     7981 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     8041 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     8101 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     8161 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     8221 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     8281 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     8341 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     8401 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     8461 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     8521 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     8581 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     8641 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8701 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8761 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8821 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     8881 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     8941 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     9001 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     9061 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     9121 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     9181 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     9241 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     9301 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     9361 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9421 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9481 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9541 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9601 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9661 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9721 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9781 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9841 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     9901 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     9961 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    10021 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    10081 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    10141 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
    10201 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttt
//
