LOCUS       GIG_270                11120 bp    DNA     circular SYN 29-SEP-2020
DEFINITION  .
ACCESSION   pVec1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 20, 2025 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(166..957,958..1026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  166 ..  957 / #ccffcc
                        2:  958 .. 1026 / #ccffcc / signal sequence
                     Cleavage site after base 957"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1027..1131)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     enhancer        1397..1776
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        1777..1980
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             1994..2174
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    2221..2346
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    2839..3072
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    3599..3716
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        3761..4208
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    4225..6042
                     /label=hGRN
                     /note="color: #00ff00"
     CDS             4279..4305
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     misc_feature    6045..6604
                     /label=IRES2
                     /note="color: #a6acb3"
     CDS             join(6616..6618,6619..6621,6622..7335)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 6616 .. 6618 / #05fd14
                        2: 6619 .. 6621 / #05fd14 / 1a
                        3: 6622 .. 7335 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    7371..7959
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7842..7853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7841"
                     /translation="IEGR"
     LTR             8031..8264
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    8296..8520
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      8566..8994
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        9008..9337
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      9188..9323
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        9385..9432
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             9451..9825
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9955..10076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(10125..10141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    10149..10165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(10173..10179,10180..10197,10198..10203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 10173 .. 10179 / #ffffff / -10
                        2: 10180 .. 10197 / #ffffff
                        3: 10198 .. 10203 / #ffffff / -35"
     protein_bind    10218..10239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(10527..11115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
       61 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
      181 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
      241 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
      301 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
      361 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
      421 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
      481 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
      541 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
      601 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
      661 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
      721 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
      781 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
      841 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
      901 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      961 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1021 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1081 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1141 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     1201 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     1261 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     1321 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     1381 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     1441 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1501 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1561 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1621 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1681 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1741 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1801 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1861 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1921 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     1981 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2041 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2101 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2161 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     2221 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     2281 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     2341 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     2401 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     2461 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     2521 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     2581 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     2641 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     2701 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     2761 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     2821 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     2881 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     2941 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     3001 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     3061 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     3121 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     3181 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     3241 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     3301 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     3361 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     3421 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     3481 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     3541 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     3601 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     3661 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     3721 tttattacag ggacagcaga gatccagttt ggttaattaa agtgcaagtg ggttttagga
     3781 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
     3841 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
     3901 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
     3961 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc
     4021 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg
     4081 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg
     4141 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga
     4201 gagcgcagac cggctcgagc caccatgtgg accctggtga gctgggtggc cttaacagca
     4261 gggctggtgg ctggaggtta cccatacgat gttccagatt acgctggtac gcggtgccca
     4321 gatggtcagt tctgccctgt ggcctgctgc ctggaccccg gaggagccag ctacagctgc
     4381 tgccgtcccc ttctggacaa atggcccaca acactgagca ggcatctggg tggcccctgc
     4441 caggttgatg cccactgctc tgccggccac tcctgcatct ttaccgtctc agggacttcc
     4501 agttgctgcc ccttcccaga ggccgtggca tgcggggatg gccatcactg ctgcccacgg
     4561 ggcttccact gcagtgcaga cgggcgatcc tgcttccaaa gatcaggtaa caactccgtg
     4621 ggtgccatcc agtgccctga tagtcagttc gaatgcccgg acttctccac gtgctgtgtt
     4681 atggtcgatg gctcctgggg gtgctgcccc atgccccagg cttcctgctg tgaagacagg
     4741 gtgcactgct gtccgcacgg tgccttctgc gacctggttc acacccgctg catcacaccc
     4801 acgggcaccc accccctggc aaagaagctc cctgcccaga ggactaacag ggcagtggcc
     4861 ttgtccagct cggtcatgtg tccggacgca cggtcccggt gccctgatgg ttctacctgc
     4921 tgtgagctgc ccagtgggaa gtatggctgc tgcccaatgc ccaacgccac ctgctgctcc
     4981 gatcacctgc actgctgccc ccaagacact gtgtgtgacc tgatccagag taagtgcctc
     5041 tccaaggaga acgctaccac ggacctcctc actaagctgc ctgcgcacac agtgggggat
     5101 gtgaaatgtg acatggaggt gagctgccca gatggctata cctgctgccg tctacagtcg
     5161 ggggcctggg gctgctgccc ttttacccag gctgtgtgct gtgaggacca catacactgc
     5221 tgtcccgcgg ggtttacgtg tgacacgcag aagggtacct gtgaacaggg gccccaccag
     5281 gtgccctgga tggagaaggc cccagctcac ctcagcctgc cagacccaca agccttgaag
     5341 agagatgtcc cctgtgataa tgtcagcagc tgtccctcct ccgatacctg ctgccaactc
     5401 acgtctgggg agtggggctg ctgtccaatc ccagaggctg tctgctgctc ggaccaccag
     5461 cactgctgcc cccagggcta cacgtgtgta gctgaggggc agtgtcagcg aggaagcgag
     5521 atcgtggctg gactggagaa gatgcctgcc cgccgggctt ccttatccca ccccagagac
     5581 atcggctgtg accagcacac cagctgcccg gtggggcaga cctgctgccc gagcctgggt
     5641 gggagctggg cctgctgcca gttgccccat gctgtgtgct gcgaggatcg ccagcactgc
     5701 tgcccggctg gctacacctg caacgtgaag gctcgatcct gcgagaagga agtggtctct
     5761 gcccagcctg ccaccttcct ggcccgtagc cctcacgtgg gtgtgaagga cgtggagtgt
     5821 ggggaaggac acttctgcca tgataaccag acctgctgcc gagacaaccg acagggctgg
     5881 gcctgctgtc cctaccgcca gggcgtctgt tgtgctgatc ggcgccactg ctgtcctgct
     5941 ggcttccgct gcgcagccag gggtaccaag tgtttgcgca gggaggcccc gcgctgggac
     6001 gcccctttga gggacccagc cttgagacag ctgctgtaat gagatccgcc cctctccctc
     6061 ccccccccct aacgttactg gccgaagccg ctggaataag ccggtgtgct ttgtctatat
     6121 gttattttcc accatattgc gtcttttggc aatgtgaggg cccggaacct ggccctgtct
     6181 tcttgacggc attcctaggg gtctttcccc tctcgccaaa ggaagcaagg tctgttgatg
     6241 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacacgctgt agcgaccctt
     6301 tgcaggcagc ggaacccccc acctggcgac aggtgctctg ggccaaaagc cacgtgtata
     6361 agatacacct gcaaaggcgg cacaacccca gtgccacgtg tgagttggat agttgtggaa
     6421 agagtcaaat ggcttcctca agcgtattca acaagggctg aaggatgccc agaaggtacc
     6481 ccatgtatgg gatctgatct ggggcctcgg tgcaatgctt tacatgtgtt tatcgaggtt
     6541 aaaaaaacgt ctaggccccc cgaaccacgg ggagtggttt tctttgaaaa acacgatgat
     6601 aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     6661 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     6721 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     6781 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     6841 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     6901 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     6961 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     7021 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     7081 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     7141 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     7201 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     7261 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     7321 gagctgtaca agtaatagaa ttcaattcta actagacgcg ttaagtcgac aatcaacctc
     7381 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7441 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7501 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7561 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7621 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7681 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7741 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7801 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7861 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7921 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     7981 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     8041 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     8101 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8161 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8221 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     8281 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     8341 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     8401 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     8461 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     8521 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8581 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8641 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8701 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8761 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8821 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8881 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8941 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     9001 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9061 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9121 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     9181 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9241 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     9301 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     9361 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     9421 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     9481 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     9541 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     9601 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     9661 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     9721 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     9781 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     9841 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     9901 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     9961 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    10021 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10081 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    10141 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    10201 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    10261 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10321 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    10381 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10441 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10501 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10561 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10621 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10681 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10741 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10801 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10861 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10921 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10981 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    11041 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    11101 aaaaaaggat ctcaagaaga
//
