LOCUS       pRS118                 12462 bp    DNA     circular SYN 20-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 20, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
COMMENT     LOCUS       pJK1027_caCas9_f       12462 bp ds-DNA     circular     
            25-MAR-2017
            DEFINITION  .
            ACCESSION   
            VERSION     
            SOURCE      .
              ORGANISM  .
            COMMENT     pACT1 is 4717-3830.tACT1 is 3642-3249.URA3 locus is 
            643-704 (piece
                        of URA3 promoter) and again 772-3154 (includes 
            orf).pJK795 insert
                        is 1554-~2795
            COMMENT     
            COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     source          1..12462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    329..828
                     /label=3' Neut5L frag
     misc_feature    329..578
                     /label=5' 250 bp frag
     misc_feature    837..1336
                     /label=5' fragment NeutL5
     misc_feature    1037..1336
                     /label=3' 300 bp block clone reverse direction
     misc_feature    1342..1591
                     /label=SNR52 promoter
     misc_feature    1618..1696
                     /label=sgRNA tail
     misc_feature    1618..1696
                     /label=sgRNA tail(1)
     misc_feature    2942..4154
                     /label=NAT with Promoter and Term
     misc_feature    3338..3910
                     /label=NATr
                     /note="NATr"
     misc_feature    4636..5029
                     /label=ACT1 terminator
                     /note="ACT1 terminator"
     misc_feature    5087..9226
                     /label=caCas9
     misc_feature    complement(5087..5669)
                     /label=gene block 5
     misc_feature    complement(5622..6371)
                     /label=gene block 4
     misc_feature    complement(6329..7327)
                     /label=block3
     misc_feature    complement(7286..8284)
                     /label=block2
     misc_feature    complement(8237..9226)
                     /label=gblock 1 CAcas9
     misc_feature    complement(9357..10244)
                     /label=pACT1
                     /note="pACT1"
     misc_feature    10656..11323
                     /label=pBR322 origin
     misc_feature    11471..12331
                     /label=AmpR
                     /note="AmpR"
ORIGIN
        1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg aaggacgatg aaggagagaa agtagtaaga
      361 caatatgact ttggacagat atgagataaa agttttaaag gacaagaaaa ggttcgggtt
      421 aaaagatgag tattgtattt tttcaaaata tcaatgcttt ttctgttctt gggttagtgg
      481 taccaccatt ggtgataaaa cctttgggga gcctcctccg agctttctct ttgttacttt
      541 ttttctcaac tgatacttgt cattcttact acaattactt tttttttttt ctccagagta
      601 tatgaaaaga tgaattttga tctcggattc aaccttttca tagaagtaat attacttctt
      661 agtaccataa tggaactaaa tcctatcaag tgattcagca ttcttcattt catgtttgat
      721 ttatgaatat atgaatatat ttttctgggt attattatac aaagcgaatt gctttttttt
      781 cttttactta attgttatat acaaatttct ttttttgtta taactatttt aattaagaat
      841 ttctagtcac ttgacacgac cttggagtga tcttttttct ttcaaatttt caattttgtg
      901 attcttagtt aattattatt aaagattatc atgaatttta atttttaaat tctaatacac
      961 gtttacttaa ttcttctctt cttctctttc tcttctcttt ctttctttct ttttattcca
     1021 tatatcatta caactataac ccactgaatt ctacatcgaa caagagaaac cttttttttt
     1081 acttttttca aacttgaaca aagaaaagaa aaagaaaaag aaaaagcaat cacagcttat
     1141 taacgattcg tgtttaaata atcaattgaa aggaaaatag aagatttttg aaaaaaagtt
     1201 attggtattt attaacttaa tatatcagta acatcaagag aaagaaactg aattataaac
     1261 agagtatgtg aagcaattgc aattgcaatt attagagatc cagaaaactg aattgtgctt
     1321 gaataccact tgtttagccg gtaaaagtca ttggtcaata actatactcg agtattgcct
     1381 catcaaagaa acaatcaaat attatagata ctcactccat cacgtgataa tttcactggt
     1441 atggaaaagt ggaaaatttt ataaaaaaaa atttgatgcc tttggcatag ctgaaacttc
     1501 ggcccaatag gattggagaa tatgttttcg cagcgttctt acaattaaat tgtggtggaa
     1561 gttcgagact tgcgtaaact atttttaatt tcgaggaaga gcattaatgg ctcttcagtt
     1621 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     1681 accgagtcgg tggtgctttt ttttgccggc gaacgtggcg agaaaggaag ggaagaaagc
     1741 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     1801 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctacgcaa
     1861 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaagggggg
     1921 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     1981 aacgacggcc agtgaattgt aatacgactc actatagggc gaattggaga ctaataggaa
     2041 ttgatttgga tggtataaac ggaaacaaaa aaaagagctg gtactacttt ctttaaaatt
     2101 attttattat ttgattttat ttaatagtat atattatatt ttgaacgtag attattttgt
     2161 tgaaagttgc tgtagtgcca ttgattcgta acactaattc tgtattagtc attcctcttg
     2221 tttgatagta tccaaaaaaa cggctatttt tttgcaatct tatttcctgc atattataca
     2281 gataacataa tgaaagaaaa aatctttttt tttgttcttc aatgatgatt tcaaccattc
     2341 ttttaaacat tgatcaattc ctgagcaaca accccataca cactggttta tataccgccc
     2401 cttttacagt tgaagaaaga aatagaaata gaaatagcaa acaaaagata tgacagtcaa
     2461 cactaagacc tatagtgaga gagcagaaac tcatgcctca ccagtagcac agcgattatt
     2521 tcgattaatg gaactgaaga aaaccaattt atgtgcatca attgacgttg ataccactaa
     2581 ggaattcctt gaattaattg ataaattagg tccttatgta tgcttaatca agactcatat
     2641 tgatataatc aatgattttt cctatgaatc cactattgaa ccattattag aactttcacg
     2701 taaacatcaa tttatgattt ttgaagatag aaaatttgct gatattggta ataccgtaaa
     2761 gaaacaatat attggtggag tttataaaat tagtagttgg gcagatatta ccaatgctca
     2821 tggtgtcact gggaatggag tggttgaagg attaaaacag ggagctaaag aaaccaccac
     2881 caaccaagag ccaagagggt tattgatgtt agctgaatta tcatcagtgg gatcattagc
     2941 aatcaagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
     3001 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
     3061 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
     3121 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
     3181 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
     3241 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
     3301 catcacatcc gaacataaac aaccggatcc tacaattatg tctactactt tggatgatac
     3361 tgcttataga tacagaactt ctgttccagg tgatgctgaa gctattgaag ctttggatgg
     3421 ttctttcact accgatactg tttttagagt tactgctact ggtgatggtt tcactttgag
     3481 agaagttcca gttgatccac cattgactaa ggttttccca gatgatgaat ccgatgatga
     3541 atccgatgct ggtgaagatg gtgatccaga ttctagaact ttcgttgctt atggtgatga
     3601 tggtgatttg gctggtttcg ttgttgtttc ttattctggt tggaacagaa gattgactgt
     3661 tgaagatatt gaagttgctc cagaacatag aggtcatggt gttggtagag ctttgatggg
     3721 tttggctact gaattcgcca gagaaagagg tgctggtcat ttgtggttgg aagttaccaa
     3781 tgttaatgct ccagctattc atgcttatag aagaatgggt ttcactttgt gtggtttgga
     3841 tactgcttta tacgatggta ctgcttccga tggtgaacaa gctttgtata tgtctatgcc
     3901 atgtccataa taactcgagt cagtactgac aataaaaaga ttcttgtttt caagaacttg
     3961 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
     4021 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
     4081 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa
     4141 cgccgccatc cagtgtcgac gtttaaacga gctcgaattc ggtactatgg agaatattct
     4201 caaaaaactg ttgaaattgc taaatccgat aaggaatttg ttattggatt tattgcccaa
     4261 cgtgatatgg gtggccaaga agaaggattt gattggctta ttatgacacc tggagttgga
     4321 ttagatgata aaggtgatgg attaggacaa caatatagaa ctgttgatga agttgttagc
     4381 actggaactg atattatcat tgttggtaga ggattgtttg gtaaaggaag agatccagat
     4441 attgaaggta aaaggtatag aaatgctggt tggaatgctt atttgaaaaa gactggccaa
     4501 ttataaatgt gaagggggag attttcactt tattagattt gtatatatgt agaataaata
     4561 aataaataag ttaaataaat aattaaataa gggtggtaat tattactatt tacaatcaaa
     4621 ggtggtcctt ctagagtcga cattttatga tggaatgaat gggatgaatc atcaaacaag
     4681 agaaaatacc cgtgacgaaa ataataaaat aagttcctct gatacagaag atgaaaacaa
     4741 caacaacaag atatagaaat gccttgggtg gctattttat agtcttaact ttttaatgta
     4801 tatttgtttt gtttttttac ataataatac tttataaaag ctaagctaaa ttcaagtaaa
     4861 atatcaatct ctcaaataaa acatttttct ctttttctta aatttagttt tatatattta
     4921 taaaataaac aaagattttt ttaaaaaagt aacaagttat atatgtaata acaaaaagaa
     4981 gaataacaag aatacaaaac cagatttcca gatttccaga atttcactcg gatcccccgg
     5041 gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgagtcag acttttcgtt
     5101 ttttcttagg atccgctcgg ctatcacccc ccaattgtga caaatcgatt ctagtttcat
     5161 ataaaccagt tatggactga tgaatcaatg tggcgtctaa cacttctttt gttgaagtat
     5221 atctcttacg gtcaatggtg gtgtcaaagt acttaaacgc ggctggagct cccaagttag
     5281 tcaatgtgaa caagtggata atattttcag cttgttctcg aattggtttg tcacgatgct
     5341 tattataagc agataaaact ttatccaaat ttgcatcagc caaaataact cttttagaaa
     5401 actcggagat ttgttcaatt atctcatcca aatagtgttt atgttgttct acgaataatt
     5461 gcttttgctc attgtcttca ggggacccct tcaacttttc ataatgagat gctaagtata
     5521 agaaattaac gtactttgaa ggcaaagcta actcgtttcc tttttgcaat tctccagctg
     5581 aagccaacat tctctttctc ccattttcaa gttcaaagag agaatacttt ggcaacttaa
     5641 taatcaaatc tttcttcact tctttatagc ccttcgcttc gagaaagtca attgggtttt
     5701 tctcaaagga tgaacgctcc atgatggtaa tacctaacaa ttctttcaca gatttcaatt
     5761 ttttggattt acccttttca actttagcca cgaccaagac agaatatgcg acagtaggag
     5821 aatcaaaacc tccgtatttc ttaggatccc aatctttttt tcgggcgatc aacttgtctg
     5881 agtttcgttt tggaagaatg gattccttag agaaaccacc agtttgaact tcagtcttct
     5941 ttacaatatt aacttgaggc attgataaaa cttttctaac agtagcaaaa tctctcccct
     6001 tatcccacac aatttcaccg gtttctccat ttgtttcaat caaaggacgt tttctgattt
     6061 ccccgttagc caaggtgatt tcggtcttaa agaaattcat gatgttggag taaaagaaat
     6121 actttgcggt ggctttccca atttcttgtt ctgattttgc tatcatcttt ctgacatcgt
     6181 agactttata atcaccatac acgaattcgg attctaactt aggatacttc tttattaagg
     6241 cagttcctac cactgcattc aaatatgcgt cgtgagcatg atgataattg ttgatctctc
     6301 tcaccttgta gaattgaaaa tcctttcgga aatctgacac caactttgac tttaatgtga
     6361 tcactttgac ctctctgatt aatttgtcat tttcatcata tttagtattc attcgggaat
     6421 ctaaaatttg ggctacatgt ttagtaattt gtcttgtttc aaccaactga cgttttatga
     6481 atccagcttt atctaattcg gataatcctc ctctttcagc tttagttaag ttgtcaaatt
     6541 ttctctgagt aatcaacttt gcatttaaca attgtctcca atagttcttc attttcttaa
     6601 ccacttcttc tgatggcaca ttgtctgatt tacctctatt cttatcagat cttgttaaaa
     6661 ctttgttgtc aatggaatca tcttttaaaa atgactgagg aactatgtga tcgacatcgt
     6721 aatctgaaag tctattgata tccaactctt gatctacata catgtcacgc ccgttttgca
     6781 aatagtacaa gtaaagcttc tcgttctgta actgggtgtt ttcgactggg tgttctttta
     6841 agatttgaga acccaactct ttaattcctt cttctattct tttcattctt tcacgagaat
     6901 tcttttgtcc tttctgagtt gtttgatttt ctctagccat ttctataact atattttcag
     6961 gcttatgacg tcccataact ttcaccaact catcaacaac tttaacagtt tgtaaaattc
     7021 cctttttgat agctggagac cctgctaagt tggcaatatg ttcatgcaat gaatcaccct
     7081 gtccagaaac ctgagctttt tgaatatctt ccttaaaggt caaactatca tcatgaatca
     7141 actgcataaa gtttctgttt gcaaaaccat cacttttaag gaaatctaag atggttttac
     7201 cactttgctt atctctaata ccattaatca actttctaga taatcttccc caaccggtat
     7261 atcttcttct ttttaattgt ttcatgactt tatcgtcgaa taagtgagcg taagttttta
     7321 aacgttcttc gatcatttct ctatcctcaa acaaagtcaa agtcaataca atgtcctcca
     7381 agatatcttc gttttcttca ttatccaaaa aatctttgtc tttgataatt ttcaacaaat
     7441 cgtgataagt tcctaaacta gcattaaatc tatcctcaac cccactgatt tcaacggaat
     7501 cgaaacactc tattttctta aaatagtcct ctttaagttg tttgacagtg actttacgat
     7561 tagttttgaa taacaagtca acgatagctt tcttttgttc accacttaag aatgctggtt
     7621 ttctcatacc ttcagtgacg tatttgactt ttgttaattc attataaaca gtgaaatatt
     7681 catataacaa agaatgttta ggtaacacct tctcatttgg caagtttttg tcgaaattgg
     7741 tcattctctc gatgaatgat tgagcggagg ctcccttatc aacaacttcc tcaaaattcc
     7801 atggggtgat agtctcttca gatttacgag tcatccatgc aaatctagaa ttacctctag
     7861 ccaatggccc gacgtaataa ggaattctga aagtcaaaat tttttcaatt ttttcacgat
     7921 tgtctttcaa aaaagggtaa aaatcttcct gacgtctcaa gatagcgtgt aactcaccta
     7981 agtggatttg atgagggata gaaccgttat caaatgttct ttgcttacga agtaaatcct
     8041 ctcgattcaa cttaaccaac aattcctcag taccatccat tttttcaaga attggcttga
     8101 taaacttata aaattcttct tgactagcac caccatcaat ataaccagcg taaccatttt
     8161 tagattgatc aaagaatatt tctttatact tttctggtaa ttgttgacgt acaagagctt
     8221 tcaacaaggt taaatcttga tggtgttcat catatctttt aatcatagag gcggataaag
     8281 gagcttttgt aatttctgtg ttgactctca aaatgtcact taacaaaatt gcatcactca
     8341 agttcttagc tgccaagaat aaatctgcat attgatcacc aatttgggca agaagattat
     8401 ctaaatcgtc atcgtatgta tccttagaca attgaagttt agcatcctcg gccaaatcaa
     8461 agtttgattt gaaatttggg gtcaaaccca aagataatgc aattaagttt ccaaacaatc
     8521 catttttttt ttcacctggc aattgggcaa taagattttc caaacgtcta ctttttgaca
     8581 atctagcaga taaaatagct ttagcatcca cacctgaggc attgattgga ttttcctcga
     8641 aaagttggtt gtaagtttga accaattgaa taaacaactt gtcgacatcg gaattatctg
     8701 gattcaaatc accttcaatt aagaagtgac ctctgaactt gatcatgtga gccaatgcca
     8761 aatatataag tcttaagtca gctttatcag tggagtcaac taattttttt ctcaaatgat
     8821 aaattgtagg atatttttcg tggtaagcca cttcatctac aatattacca aagattggat
     8881 gtctttcatg tttcttatct tcttcaacta agaaactttc ttccaatcta tgaaaaaaag
     8941 aatcatcaac ctttgccatt tcattagaaa agatttcttg caagtaacaa attctgttct
     9001 ttcttctggt atatcttctt ctagctgttc ttttcaaacg agttgcttca gctgtttcac
     9061 cactgtcaaa caacaaggca ccaattaaat tctttttaat ggagtgtcta tcagtatttc
     9121 ctaaaacttt gaacttttta gaaggaacct tatattcatc agtgattaca gcccatccaa
     9181 ctgaattggt tccaatgtcc aaacctatgg agtatttttt atccattttg aatgattata
     9241 tttttttaat attaatatcg agataatgtt tctaagaaag aaagaaaacc aggagtgaaa
     9301 attagaaaag gaaaggaaag gaaaaaaaga aaaatctgaa aatatataaa aaaaaattgt
     9361 ttcgttggca ataaatcctg gtgagaacag cgaccgaaag caaataagaa caaaatataa
     9421 gtgtattacg ttgaacaact aattaacgtg tgtgtatgga tctttttttc ttttttctct
     9481 ttaaccgact ataaacaaca aacatttttg ggcagtgcac acacactact taatatacac
     9541 agcataaatt acacgattag aaaacaaatt agcttattaa aataacctaa tcaaaccgaa
     9601 tattttatgg tattacgagt aaactatata atataaatag cacacaccca caacaacaac
     9661 aaaggaaaac taaaaggttt tttctttttg aaaagatcgt tttctttatt attctctagt
     9721 tttgacgctg aaatattcgt tgtactgttg caaatttgta tttggtgtgc cttatcttct
     9781 tttctacact ttttacttat gagctttata attcacagat aagtttcagg tttgccagat
     9841 atacttgctt cactggttac taaactctcg gtgtacaaat gacttgctta tgtaagaggc
     9901 tacttttcta gttttgcagt cattcattta aaaacaattg atcctgaagg ctctaaagac
     9961 atagtaggtt tactttgact gtatatttcc gtggcatgtt taaaggcccc ttggccatag
    10021 gatattggtg gcggcacagt aagcattaaa attgttgttt cgcggaatta caacatttct
    10081 tcttttacat aataatgtca ctcctttttg attggcatag ccgagatctt tttttaacac
    10141 agactttaag ctgttttttg ccacctgaga acagttctat tttggtccaa aagaaatatt
    10201 agactaagtt tattataaac gaggctggtg atcttaatag ctctggtacc ctccaattca
    10261 gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg tcatagctgt
    10321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cataggagcc ggaagcataa
    10381 agtgtaaagc ctggggtgcc taatgagtga ggtaactcac attaattgcg ttgcgctcac
    10441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    10501 cggggagagg cggtttgcgt attgggcgct cttacgcttc ctcgctcact gactcgctgc
    10561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctcggcccc cctgacgagc
    10741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10801 aggcgttccc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
    10921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    11041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    11101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    11161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11341 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11401 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    11461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    11521 gttcatccat agttgcctga ctgcccgtcg tgtagataac tacgatacgg gagggcttac
    11581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    11701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    11761 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11881 gaaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11941 tgttatcact catgcttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    12001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    12061 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    12121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    12181 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    12241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    12301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    12361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    12421 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tg
//
